FastQCFastQC Report
Sun 19 Mar 2023
SRR6155062_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155062_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109955356
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCTA3346100.0139.283311
ACGTGAT2977950.0138.516272
ACGTATC2223450.0137.92271
GTCACTA2540450.0137.58282
AACGTGA3010600.0136.88881
AACATCG3391800.0136.507262
GAGCTGA2892450.0136.209171
CCGAAGT2550100.0135.595111
TGCCTAA3251250.0135.50582
CAAGCTA2765500.0135.458182
ACGCTTA2322300.0135.021512
AGTCACT2872850.0134.893591
CGAAGTA2387200.0134.540312
CGCATAC2031250.0134.172561
GCTAGTC1017200.0133.92213
CGACTGG2249000.0133.64721
CTCAATG1444700.0133.470151
CTGGCAT1497200.0133.412611
AGCTGAA2637200.0133.129042
GCCTAAT1337950.0132.333833
Produced by FastQC (version 0.11.5)