FastQCFastQC Report
Sun 19 Mar 2023
SRR6155060_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155060_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104624436
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2590910924.763917484821615No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGGACGGGTGGCGTGTG22929742.191623761775882No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA18152911.7350545144157337No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA7322810.6999139283293245No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG5131030.49042367119666No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA4858480.4643733515562272No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3849550.36793985680362473No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCATCGGCTGGCACCAC3581580.3423272934059114No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG3459020.33061301281471184No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG3392510.32425598929871413No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT3332530.31852310295847136No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAGTACA3312390.3165981224500938No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT3269110.3124614215363608No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC3125370.298722757272498No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT3115000.2977315930286114No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAGTTGAGTGCTGAAAAA2940780.2810796514114542No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA2818080.26935198962506235No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG2806890.26828244980933513No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA2775120.2652458743003403No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2639360.2522699381624385No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC2610600.24952105835007796No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTACATGGGAAGCAGTGG2443360.23353626489322243No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG2436140.23284617754116255No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA2377940.22728342353979333No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTACATGGGAAGCAGTGGTA2310270.22081552726363082No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA2307370.22053834536321895No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAGTAC2238770.21398155971899335No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGAGTACATGGGAAGCAGT2170180.20742572987442437No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2135260.2040880774736028No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC2027670.19380462896832246No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT2024070.19346054109194913No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGCAGTGGTATCAACGCAG1914210.18296012606462222No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1907930.18235988388028204No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCA1903520.18193837623172468No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1835240.17541217617651003No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG1822010.17414765323083797No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC1813900.17337249970934132Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAACGCAGAGTACATGGGA1738670.1661820188927948No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGTAAGTTAGAAAAAAAA1705270.16298964803977534No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC1609700.15385507072171936No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTACATGGGAAGCAGTGGTAT1491050.14251450779624752No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA1451840.13876681734274773No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1414800.13522653541472854No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA1393980.13323656052970262No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGA1388490.13271182651823327No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCTG1328130.12694261979104002No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1323910.1265392723359579No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTACATGGGAAGCAGTGGT1248760.11935643791666413No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1244110.11891199107634855No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG1240180.11853636181130764No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAAAAA1112900.10637094378219634No Hit
TACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT1077090.10294822521193807No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGCAGTGGTATCAACGCA1070300.10229923724511165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT190036400.043.875371
AGCAGTG196512650.042.4602282
AGTGGTA196953550.042.3337975
CAGTGGT199934650.041.713354
GTATCAA200501150.041.608989
GCAGTGG200911000.041.5100633
GGTATCA203554050.040.972788
TGGTATC203886500.040.895737
GTGGTAT204053500.040.860186
GACTGGA442700.027.4891623
TGACTGG453000.027.1186182
AGTTCAG481300.025.130799
GTGACTG509050.024.2210561
CTGGAGT555300.022.1837445
TGGAGTT569450.021.5440086
ACTGGAG605200.020.1539024
GAAACCG389850.018.49764685-89
AAACCGC398800.017.88031685-89
CGTAAGT514250.017.70790930-34
CCGTAAG520300.017.5933330-34
Produced by FastQC (version 0.11.5)