FastQCFastQC Report
Sun 19 Mar 2023
SRR6155058_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155058_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126709122
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1579540.12465874398529886No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCACGGACGCGGCGTTGATAC1342580.10595764368093405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC3200350.0139.106931
ATGCCTA2579000.0138.792661
AGATCGC3756700.0137.449891
CAATGGA7871150.0136.411621
CAAGCTA3444550.0136.353332
GATCGCA3189100.0136.152282
ACGCTTA2411550.0136.02462
GAGCTGA2774000.0135.597931
ACGTGAT2111650.0135.574832
CTGGCAT2101700.0134.716831
CCGAAGT2461750.0134.576871
AACATCG2525200.0134.06242
TGCCTAA2372950.0133.871282
GTCACTA1569100.0133.838732
CTCAATG1628700.0133.714081
AACGTGA2147150.0133.42851
AGTCACT1882150.0133.423771
CGCATAC2065350.0133.289811
ACTTCGA1424250.0133.281222
ACAAGCT4155850.0133.043011
Produced by FastQC (version 0.11.5)