FastQCFastQC Report
Sun 19 Mar 2023
SRR6155058_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155058_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126709122
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1980878715.633276189854744No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGGACGGGTGGCGTGTG28168432.223078303707289No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA13729021.0835068370215684No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCATCGGCTGGCACCAC7172670.5660736880490735No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5191840.40974476959914535No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGTAAGTTAGAAAAAAAA4763170.3759137404487737No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG4696970.3706891757958831No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA4543550.3585811288314349No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAGTTGAGTGCTGAAAAA3570320.28177292555148475No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG2720540.2147075093772649No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG2636230.20805368693186904No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2373240.18729827517864106No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTAAGTTAGAAAAAAAAA2252060.17773463855270027No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2161050.1705520459687188No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2094880.16532984894331443No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAGTACA1623220.12810600960521218No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1587980.1253248365180843No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1576620.12442829490997499No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT1548810.1222335042302637No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTGGGTAAAAAAAAAAAA1524410.12030783387481762No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1486330.11730252538566244No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1453070.11467761571262405No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1396070.11017912348883611No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAGTAC1361950.10748634182785988No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA1276130.10071334879899174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT147772900.059.5416641
AGCAGTG151135300.058.2682382
AGTGGTA151301400.058.1605645
GTATCAA152375900.057.824259
CAGTGGT152965850.057.539424
GCAGTGG153664300.057.28393
GGTATCA154037300.057.196488
GTGGTAT154674500.056.9446076
TGGTATC154840150.056.9074027
GAAACCG1071200.021.82219985-89
ATGGGGC3597800.021.74518225-29
CATGGGG17263100.021.52140225-29
AAACCGC1078200.021.47218385-89
ATGGGGG6169050.020.99111625-29
GACTGGA728550.020.6949543
CGTAAGT1121900.020.37707930-34
CCGTAAG1133150.020.33870330-34
GTGACTG754350.020.191181
TGACTGG776700.019.5975062
GCCGTAA1175250.019.46922330-34
Produced by FastQC (version 0.11.5)