FastQCFastQC Report
Sun 19 Mar 2023
SRR6155057_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155057_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117194745
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAGC6939250.0141.806141
ATCAGCA6012500.0139.842832
TCACGCA4644450.0139.702472
TTCACGC5471350.0139.202621
TAGCGAC2338650.0138.841382
GATAGAC3540300.0138.824571
ATAGCGA2571300.0138.330961
CTAACGA8020800.0137.115812
CCGTCTA3648700.0137.002852
GTCGTAG2030500.0136.076251
GGCTAAC3281250.0135.771872
GCGAGTA2824000.0135.636461
CGACAAC6522750.0134.979972
TCAGCAT2057800.0134.922153
CCGACAA7230000.0133.751081
TCAGCAG2080100.0133.662613
ATCATTC3757400.0133.539641
GTCTGTC2864200.0133.526351
CACGCAT1615350.0133.24743
ATAGACA3260250.0133.077642
Produced by FastQC (version 0.11.5)