FastQCFastQC Report
Sun 19 Mar 2023
SRR6155056_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155056_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116588330
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCTA4390400.0141.000441
AGTCACT4100650.0140.04871
GAATGCG5600350.0139.9091
ACGTATC3271750.0139.866411
GTCACTA3535500.0139.855132
ATGCGTC2882600.0139.256643
CAAGCTA4787450.0138.953172
TGCCTAA4013550.0138.308842
GACTAGT3030100.0137.872991
AATGCGT4000550.0137.644262
ACGCTTA2551950.0137.403842
ACGTGAT2216100.0137.073882
CGCATAC2941350.0136.984511
ACAAGCT5637300.0136.536911
CCGTGAG3303950.0135.986481
AACGTGA2239700.0135.033621
CCGAAGT2230800.0134.893421
ACTAGTA2686450.0134.509832
CTCAATG1399400.0134.349961
CGAAGTA1954200.0134.042282
Produced by FastQC (version 0.11.5)