FastQCFastQC Report
Sun 19 Mar 2023
SRR6155054_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155054_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97583809
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCTA3078800.0139.137191
GCTCATG3865250.0138.835141
TCATGTC3084200.0137.166033
ACGCTTA2871200.0137.047882
ACGTATC2041600.0136.721821
TGCCTAA2775300.0136.176832
CTCATGT3752900.0136.162842
AGATCTG3347850.0134.368732
CAAGCTA2493400.0134.355482
ACGTGAT1597400.0134.065952
GAGCTGA1970550.0133.747991
AACATCG2128800.0133.70292
CCGAAGT2035650.0133.548081
CTGGCAT1743400.0133.520251
CAGATCT3370250.0133.347441
CGAAGTA1773250.0132.532442
GTCACTA1374950.0132.288682
AGTCACT1629800.0132.055711
CTCAATG1258600.0131.825551
CATCAAG1764350.0131.656191
Produced by FastQC (version 0.11.5)