FastQCFastQC Report
Sun 19 Mar 2023
SRR6155052_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155052_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104638093
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGAG7988600.0138.484021
CGTGAGA6630350.0137.818652
CGAACTT5194950.0136.264251
CTCAATG2590300.0135.676131
ACGTATC2086000.0135.260771
ATGCCTA1774500.0135.20131
GACTAGT2958600.0134.878421
CTACGTC1724350.0134.759673
ACGTGAT2224650.0134.740172
CTGGCAT2303100.0134.660491
AACATCG2972150.0134.445052
AGATCGC2576750.0134.349471
CGCATAC2218600.0134.100021
GATCGCA2250050.0133.907322
TCTACGT2103450.0133.082032
CTCTACG2132200.0133.073261
ACTAGTA2656350.0132.917242
GAACTTA4625750.0132.778212
CGACTGG2093600.0132.559591
AACGTGA2249000.0132.529621
Produced by FastQC (version 0.11.5)