FastQCFastQC Report
Sun 19 Mar 2023
SRR6155051_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155051_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121095093
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA4953550.0138.423051
TAGCGAC4491200.0138.371282
TATCAGC2401950.0136.043641
TGGCTTC7695850.0136.03821
GGCTTCA6806350.0135.948352
GGCTAAC3990650.0135.91312
TCACGCA3288000.0135.749132
GATAGAC2448000.0134.67561
AGTTAGC2436800.0134.450912
TTCACGC3801050.0134.308441
GCTTCAG2354350.0133.06143
ACCTTAC3639100.0132.731052
GTCTGTC2590750.0132.639341
AGGCTAA4561650.0132.55371
ATCAGCA2185950.0132.541032
ATCCGTC1665400.0132.185062
GTCGTAG1611650.0132.053531
AGCGACT1533550.0131.650073
ATCATTC3448950.0131.47631
CCTAATC2490350.0131.123551
Produced by FastQC (version 0.11.5)