FastQCFastQC Report
Sun 19 Mar 2023
SRR6155050_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155050_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118443991
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC5343190.45111532926984876No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT2899310.244783207279802No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT2075670.17524485476008655No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGGACTCTGCGTTGATAC1315960.11110399007071621No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTGA9070950.0140.941211
ATGCCTA3472500.0140.546891
GTTACCG7127450.0140.446671
AGCTGAA7849400.0139.852262
TTACCGT4353550.0139.203832
TACCGTC2874400.0138.838043
TGCCTAA3196250.0136.797262
CTCAATG1973600.0136.69371
GCTGAAT3051350.0136.001113
AACATCG2801550.0135.384372
TTACCGG3054750.0135.222
ACGTATC1542700.0134.80641
GCTGAAG2652850.0133.66123
GCCTAAT1343400.0133.60953
ACTTCGA1287900.0133.124152
ACTAGTA1517600.0133.100592
CTGGCAT1735500.0132.777511
CAAGCTA1832450.0132.760362
GACTAGT1768650.0132.372271
ACGCTTA1649100.0131.992162
Produced by FastQC (version 0.11.5)