FastQCFastQC Report
Sun 19 Mar 2023
SRR6155049_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155049_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119643132
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2722090.22751744747036545No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA1743180.14569829215102795No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGGACTCTGCGTTGATA1243540.10393743286493036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA3196100.0139.677461
TATCAGC3240300.0139.654421
TAGCGAC2951750.0139.535252
AGTTAGC3276550.0138.948552
TCACGCA2867050.0137.321272
GCCACAT4117600.0137.100811
ATCAGCA2909100.0136.584272
ATCATTC3637800.0135.268511
TTCACGC3412250.0135.150181
GGCTAAC2472250.0135.12312
GTCTGTC2886250.0134.869671
GAGTTAG3687750.0133.762791
GTCGTAG1729150.0133.688251
ATGAATC2816600.0133.49492
ATCCGTC1654150.0133.005742
TAAGGTC2016100.0131.789442
CCTAATC2027650.0131.568021
AGGCTAA2801300.0131.179731
TCGTAGA1497450.0130.867462
GCGAGTA1935600.0130.725831
Produced by FastQC (version 0.11.5)