FastQCFastQC Report
Sun 19 Mar 2023
SRR6155048_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155048_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93095682
Sequences flagged as poor quality0
Sequence length150
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2642860.2838864212842868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATG2405550.0139.116461
ACTTCGA2507300.0138.80782
ATGCCTA1973750.0138.758541
ACCTTAG4065850.0137.631471
CGCATAC4153850.0137.35941
CTTAGTC1925150.0137.292653
CGAACTT2194400.0136.993351
CCGAAGT2512150.0136.841171
CCTTAGT2640800.0136.427922
CACTTCG3112250.0135.780561
TGCCTAA1901600.0135.37362
CTGGCAT1704000.0135.11561
CAGTTCA1963200.0134.564062
CGAAGTA2188850.0134.520652
GAGCTGA2268350.0133.948171
TGGCATA1482000.0133.805392
CTTAGGC1298200.0133.736363
CCGTGAG3186000.0133.271181
CCTTAGG1720500.0132.456742
AGCTGAA1902250.0132.430372
Produced by FastQC (version 0.11.5)