FastQCFastQC Report
Sun 19 Mar 2023
SRR6026879_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026879_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204145115
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG67230593.293274492509899No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA19501200.9552616529668123No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG6494510.3181320307370568No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG6157950.3016457190268795No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC4804540.2353492514381253No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2799730.13714410947330286No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2770580.1357162036426882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT153296450.0113.2273251
AGCAGTG156967550.0110.5747152
GTATCAA157033600.0110.5665059
AGTGGTA157032550.0110.5574955
GGTATCA157304500.0110.289888
TGGTATC157522750.0110.1307757
GTGGTAT157734650.0110.011856
CAGTGGT157820700.0110.00524
GCAGTGG160361000.0108.252643
CATGGGG29887250.025.56064425-29
CATGGGT15396350.024.96602625-29
CATGGGC19627450.024.7822125-29
ATGGGAT18270900.024.45878225-29
ATGGGAC9168300.024.4125325-29
ATGGGGG10087850.023.99337425-29
ATGGGCG3167550.023.84816225-29
ATGGGAG19590600.023.6352725-29
ATGGGGT6763050.023.61896725-29
ACATGGG144603300.023.42605220-24
TAGCGAG1547200.023.25686130-134
Produced by FastQC (version 0.11.5)