FastQCFastQC Report
Sun 19 Mar 2023
SRR6026875_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026875_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144273908
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG127935218.867522324272246No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA29544592.0478124152566797No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG12047330.8350317924430244No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC9367350.649275404669845No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA9191510.6370874766905185No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA8002270.5546581575928476No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC6215060.43078198172880994No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT5844220.40507809631108077No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACACTCGCTTCTGGAACG4108730.28478676823532084No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTCTTCTGGTCCCCACAG2720040.18853305061924294No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA2317520.16063334196229023No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTCTTGCTTCAAC2177650.15093858828583195No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACACTCGCTTCTGGAACGT2066570.14323934442809994No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTCTTGCTTCAACA1490970.10334301057402563No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG1478470.1024766030459229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT133470000.080.769521
AGCAGTG137758750.078.256742
AGTGGTA137432550.078.254615
GTATCAA137467050.078.009089
CAGTGGT138763350.077.490534
GGTATCA138840400.077.342978
GTGGTAT139401250.076.96916
TGGTATC139720150.076.812187
GCAGTGG142231350.075.579223
ACACTCG1972000.025.537830-34
CATGGGG22027750.024.65105425-29
ATGGGGG8471400.024.38658525-29
TGCCGAC1579350.024.03138580-84
GGTCGGC1589750.023.98455115-119
ACGTCAA1536350.023.89112395-99
CTAGTCC2097350.023.8349870-74
GTGATAT752250.023.7351266
CGCTTCT2158650.023.68449235-39
GAAACCG2486900.023.66580685-89
CGTACGG267700.023.40844550-54
Produced by FastQC (version 0.11.5)