FastQCFastQC Report
Sun 19 Mar 2023
SRR6026868_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026868_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166735062
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG20336761.219705067192166No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG7368100.4419046547030402No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA5764730.3457419171979556No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC3696990.22172840886939543No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG2388830.14327100559089365No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC2092070.12547270951325162No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1915740.11489724938597498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT120762300.0131.230641
TGGTATC122441150.0129.080547
GGTATCA122593650.0128.93738
GTGGTAT122625300.0128.931736
GTATCAA122845700.0128.802129
AGTGGTA122861250.0128.74565
AGCAGTG123182050.0128.561042
CAGTGGT123235200.0128.499924
GCAGTGG125775200.0125.87653
ACATGGG118463300.026.49182920-24
CAACGCA122508750.026.3800510-14
ATCAACG122394400.026.32500310-14
TCAACGC122667650.026.31228310-14
AGAGTAC122596950.026.3063515-19
ATGGGAT22052800.026.30164125-29
TACATGG122081200.026.27250320-24
GTACATG122376100.026.24840220-24
AGTACAT122588600.026.24498720-24
CATGGGG23509000.026.23546225-29
CAGAGTA122956800.026.23527515-19
Produced by FastQC (version 0.11.5)