FastQCFastQC Report
Sun 19 Mar 2023
SRR6026865_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026865_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231553101
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG216370249.344303274953765No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA67878042.931424356091867No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG11061420.47770554366274715No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC5234440.2260578665279892No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA5194530.22433428779690584No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA2920100.12610930224596734No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2647220.11432453241038651No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG2458370.10616873578384943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT245016200.075.387251
AGTGGTA250232200.073.789955
AGCAGTG250620000.073.6870962
CAGTGGT251015950.073.567464
GTATCAA251705800.073.3839
TGGTATC251563800.073.339327
GTGGTAT251698000.073.303256
GGTATCA252123250.073.219778
GCAGTGG255343850.072.308513
CATGGGG29046750.023.26596625-29
ATGGGAT19247500.022.69480325-29
ATGGGGG9741250.022.35800725-29
AAACCGC2527100.022.07882185-89
ATGGGGC6219400.021.9444725-29
ATGGGGT6038150.021.92188625-29
ATGGGAC9131600.021.90833725-29
CATGGGC18045300.021.77680625-29
CATGGGT11541900.021.59208325-29
GAAACCG2622900.021.5718385-89
ATGGGCT6213100.021.55417425-29
Produced by FastQC (version 0.11.5)