FastQCFastQC Report
Sun 19 Mar 2023
SRR6026863_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026863_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163952291
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG30388251.853481266693614No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG5395020.329060360614296No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA4346890.26513139727946833No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA3298260.20117193726801905No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC3010660.1836302488752658No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2594850.15826860266319792No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG2357450.14378878060325487No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC1840100.11223386930286933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT133177000.0129.904341
TGGTATC135764300.0127.4513857
GTGGTAT135958000.0127.249896
AGTGGTA136049500.0127.201895
GGTATCA136075650.0127.18778
GTATCAA136019100.0127.173889
AGCAGTG136079300.0127.1231162
CAGTGGT136364000.0126.901444
GCAGTGG138720600.0124.715793
ATGGGAC13104950.026.61358625-29
ATGGGAT25166450.026.47025525-29
ATGGGAG29233500.026.4469325-29
ACATGGG132698500.026.0709720-24
CAACGCA135371200.026.03568310-14
AGAGTAC135262050.026.0341715-19
ATCAACG135622750.025.98674610-14
TACATGG135380300.025.98342720-24
CAGAGTA135575300.025.97908415-19
AGTACAT135537800.025.97066520-24
CATGGGG21303750.025.96136925-29
Produced by FastQC (version 0.11.5)