FastQCFastQC Report
Sun 19 Mar 2023
SRR6026856_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026856_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234062546
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2930698412.521005389730316No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA34456281.4720971205704991No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG9633970.4115981033548187No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA6599930.2819729218872976No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC4952630.21159429753447181No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA3241540.138490333263315No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT2536010.10834753544892227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT269490750.076.3836061
AGTGGTA274974300.074.845015
AGCAGTG275243650.074.756592
CAGTGGT275626550.074.6613544
GTATCAA276112050.074.564659
GGTATCA276634600.074.379888
TGGTATC276533350.074.3773967
GTGGTAT276642750.074.363246
GCAGTGG279024100.073.743763
ATGGGAT23325600.023.89861725-29
CATGGGG27296750.023.66652925-29
ATGGGAG29055050.023.37605325-29
ATGGGGG9613100.023.36344325-29
ATGGGAC11769200.022.86491625-29
ATGGGGT5677200.021.94102325-29
AAACCGC2284200.021.64339885-89
ATGGGGC5647100.021.51332325-29
GAAACCG2371200.021.16932585-89
CATGGGT10956400.021.11523425-29
TGGGATG7543050.020.8326725-29
Produced by FastQC (version 0.11.5)