FastQCFastQC Report
Sun 19 Mar 2023
SRR6026846_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026846_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217705443
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG7052627332.39527318570533No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA65275852.998356361719445No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA18406800.8454910334970357No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA12842500.5899025684902146No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG10689050.49098680550674156No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT8135270.3736824347565807No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT8119730.37296862623687366No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT7607630.34944601729594793No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG7529600.3458618166014343No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA7523150.34556554472549406No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG7153210.3285728597975384No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA6748040.30996193328983507No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA6747090.3099182963468672No Hit
AAGCAGTGGTATCAACGCAGAGTACATTGGAAGCAGTGGTATCAACGCAG6656600.30576176269511096No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC6313870.2900189316810053No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG6143750.2822047035360526No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC5898300.27093029548186354No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA5751270.2641766747191525No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5538160.2543877600708403No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT5468040.25116689434356493No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC5461550.2508687851226577No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCA5328070.24473756496754193No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG5140420.2361181203907704No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5085620.2336009577858832No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG5033520.23120781596627332No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC4503900.20688044992977048No Hit
AAGCAGTGGTATAAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4437270.20381989255087207No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG4376100.20101013275997884No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTTGTATCAACGCAG4305700.19777640561793397No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC4227790.19419771695832153Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG4182960.19213851258647677No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA4104180.18851986167383053No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA3947170.18130782334183532No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG3887220.17855410257243776No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAGTACA3853690.1770139481537905No Hit
AAGCAGTTGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3811540.17507784589473951No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATAAACGCAG3738000.1716998871727796No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAT3661690.16819469231185002No Hit
TACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT3460440.15895055044627432No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC3239200.14878819543340494No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG3182510.14618421827882364No Hit
AAGCAGTGGTATCAACGCAGAGTACATGTGAAGCAGTGGTATCAACGCAG2951470.13557171374902188No Hit
AAGCAGTGGTATCAACGCATAGTACATGGGAAGCAGTGGTATCAACGCAG2949500.13548122450939365No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCG2901770.13328881262743625No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG2659510.12216093283437107No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGAGTACATGGGAAGCAGT2471260.11351392808309345No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAGCAGTGGTATCAACGCA2379960.10932018819575402No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG2363980.10858616888140918No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACGTCTGAACTCC2355920.10821594387054438Illumina Multiplexing Index Sequencing Primer (95% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGCAGTGGTATCAACGCAG2353700.10811397122487194No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGA2248000.103258787149387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT506845100.036.629131
AGTGGTA518613250.035.7582865
AGCAGTG518173100.035.662272
GTATCAA524091550.035.359449
CAGTGGT527619050.035.090084
GCAGTGG530455200.034.8762553
GGTATCA528891750.034.87218
GTGGTAT536031550.034.6160746
TGGTATC533231950.034.60767
GGTATAA3749800.030.630188
TGGTATA3818050.030.1052767
GTATAAA3933300.029.184729
AGTTGTA3885500.027.0237125
TTGTATC3881950.026.9760957
GTTGTAT3912950.026.7752596
CAGTTGT3980650.026.3582554
GCAGTTG3996600.026.2100053
TGTATCA4039100.025.8570128
AGCAGTT4129850.025.5128362
ACACTCG338400.017.94396630-34
Produced by FastQC (version 0.11.5)