FastQCFastQC Report
Sun 19 Mar 2023
SRR6026837_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026837_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222614606
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG180659998.115370022037098No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA67544843.034160301233783No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7735160.3474686651962091No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG7507920.33726088934164544No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAGAGAGAGAGAGAGAGA3910060.17564256318383709No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA3531880.1586544595371249No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATACACATCTCGTATGC3363480.151089816631349TruSeq Adapter, Index 27 (97% over 37bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC3077050.13822318558917918No Hit
AAGCAGTGGTATCAACGCAGAGCACACGTCTGAACTCCAGTCACATACAC2687270.12071400202734227TruSeq Adapter, Index 27 (96% over 30bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC2322280.10431840218067272Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2241010.10066769832703609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT236964200.085.674541
AGTGGTA242223300.084.2345
AGCAGTG241012200.083.938972
GTATCAA243709300.083.8796549
CAGTGGT243022400.083.8620154
GTGGTAT243395950.083.7998666
TGGTATC242689000.083.753817
GGTATCA243131200.083.679548
GCAGTGG246152700.082.733993
ACGCAGT2316750.067.242531
CGGTTAA326400.051.2897031
GCAGTTG2096300.048.691773
TTGTATC2189900.046.734177
GGTATAA2002050.046.260798
TGGTATA2017700.045.898417
GTTGTAT2234850.045.7909746
CGCAGTG3529800.044.0444872
CAGTTGT2331650.043.8775834
TGTATCA2487550.041.1971448
AGCAGTT2707200.037.6097032
Produced by FastQC (version 0.11.5)