FastQCFastQC Report
Sat 31 Dec 2022
SRR5849865.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849865.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30085
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC21237.056672760511883No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCCGTCT13204.387568555758683No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGC3721.2364965929865381No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA2490.8276549775635699No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAT1570.5218547448894798No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGC1430.47531992687385743No Hit
TTGTTGGGGCGATCTGCATGCGGGTGTCCAGGAATTGGGAAGAGTGAGAGGA1380.4587003490111351No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCTTATAC1130.3756024596975237No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTA1110.36895462855243477No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCT1080.3589828818348014No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTATACAC1050.349011135117168No Hit
AAAACAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1020.3390393883995346No Hit
AGCACGGGGAATTTTCGATTTGACAGATGTAAGAAGCAGCATGAATAGTTTA1020.3390393883995346No Hit
TTTGCGGGATTCCTTGCATCTTTGGGTCTACTGAGCAGTGGGTGCTGAGGTG1000.33239155725444575No Hit
TATAAAGGGAGGGCAGGAACCCCACAGACTGTCCCTTCCAGCCCACACTCTG930.30912414824663453No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT930.30912414824663453No Hit
TAGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAG920.30580023267409007No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCC910.30247631710154566No Hit
TTTGTTGGGAAGTTTATTTGGGGACAGAAATGGCAACTTGGACCCAAAGAAA910.30247631710154566No Hit
GCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAAGCTATCGGG900.2991524015290012No Hit
AACGATGGGTCCAGCAATCTTTAGACTACAATAATACTTTTATCCATGTGCT890.29582848595645667No Hit
ATTTGCGGGCACAACTGAAAACATGAGGGACTATATCCAGTCACTTGTAGCC870.2891806548113678No Hit
GTAAAAGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA850.2825328236662789No Hit
AATCGCGGGAACAATTAGAAAGTAATTGGAGCTTGAAGTATTCTAGAAGACT790.2625893302310121No Hit
GTAATGGGGATTAGAAAACATTTAGTAAAAATCATCTCTAGTTCAAACCCTC790.2625893302310121No Hit
CTATAAGGGGTTAGTAGCCAAGCAAATGCAGTACATACTCATAAGACAGAAT740.24596975236828986No Hit
AAGCAGGGGCGACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCG730.2426458367957454No Hit
GCCTAGGGGGTGCTTAGTGATTGGTTTCCAAAGAGTAGAGTATTGAAAGTGG720.23932192122320092No Hit
TATTCAGGGATAAAACATCCATATAGGATTATAGATACTTAAAGGAACACGT720.23932192122320092No Hit
CATAATGGGAATTCAGTCCACCAAAGATTGAATTTTCTTAGACTATGTATAA720.23932192122320092No Hit
CTATTTGGGGTTTTTGCAGTATTCTATATATAATTTCCTTAATTTGTAAATT700.23267409007811202No Hit
AATGAGGGGAGGGACAAGGTGTCATCCAAGAATTCACTTGAGTCCTATGCCT680.2260262589330231No Hit
TCATGAGGGGATAACCATGCACACTACTATAACCACCCTAACCCTGACTTCC670.22270234336047864No Hit
TCATAAGGGAGTCTTAAGTAATGTGAATTCACTGTATTTCTTTTTATACAAC630.2094066810703008No Hit
ATCGCTGGGACCACAAAATGATGGAGCCAGAATTTAGTTGCAGAACTTCTGA610.20275884992521193No Hit
AACTAGGGGATTCTCAGGAGGTCTGAGTTACCACTTTAAGATAGCAGAAAAA600.19943493435266743No Hit
TCATCCTGGGAACATCAGATCTTTTCTATAATATCCTACTACTTTGGTTTTC600.19943493435266743No Hit
CTCATGAAGGGAAGGACATGAATAGATGGTCTCCTTTAAGACTTGTTAAACA590.19611101878012296No Hit
CGCTCTGGGATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAA580.19278710320757853No Hit
TACAATGGGCCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAA570.18946318763503409No Hit
TGCCAAGGGTCCCACCGGGGACTGGACACAAACAGGAATGTCTGGAGTGGTA570.18946318763503409No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCC560.1861392720624896No Hit
TGTCGCGGGATTCTAGGAAAGACTAGGGATTCATTCTTTTATTTATTCTTTT560.1861392720624896No Hit
ACTCTCGGGATTCCATGTGCTTAAATGATAATTTCCTTATTCAGTTTCAGAA560.1861392720624896No Hit
TGTCTAGGGTAGGTACAACATGGGAAAAGAATTCCCAACAAATTACACGTTT550.18281535648994515No Hit
TGTTCTGGGCGATCTAGTTATGGGAATAGAAGGAAGTGCTAAAATCAAGTTA540.1794914409174007No Hit
ATTGCTGGGCTATTCTGCCTAGCAAACTCAAACTACGAACGCACTCACAGTC530.17616752534485625No Hit
AGCCAAGGATTTGAGATCAGCCTGGGCAACACAGGAAGACCCCCTCTCTACA520.17284360977231178No Hit
CAATAAGGGGCATCCAATTTTACACTCAGTTAAAGACCATTACTTCTCAGTG510.1695196941997673No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGC510.1695196941997673No Hit
ATCGCTGGGGGGGGCCCAGGTTGGATGGGGAAAGGCTAATGGGGTCAGACCC510.1695196941997673No Hit
TGGTAAGGGACACAATCAAAGACGCCCTCGGCTTACTTCTCTTCCTTCTCTC490.1628718630546784No Hit
GCCTGGAGATTGTGAACCCACAGGCGGCAGAGAAGAAGGTGGCTGAGGCCAA490.1628718630546784No Hit
CTTTTAGGGGAAGCTTTGCTCATTCAAACAGATGAAGGCAAGAGTATTGCTA490.1628718630546784No Hit
TTTTCAGGGATCCTTAGATCTTTGCAAATTTCCAAATTACTCAATACCACCA470.1562240319095895No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAA470.1562240319095895No Hit
GTAATGGGGAGAGATTATAATATTATAATTGTAACACAGTTAAATATTATAT460.15290011633704503No Hit
GCGAAAGGGGAAAACACAGAAGAAAAAAATACATAAGAAAATTGAAGTCACC460.15290011633704503No Hit
ATAAATGGGATATTCTGACTTGGCTATTGAGGGGAAAATTCAGTATTAAGTG460.15290011633704503No Hit
CACGTGGGGGAACTATTTAACATATAAATGGGGACACAAAACCTGAATTGTG460.15290011633704503No Hit
GTTCTAGGGCAGTAAGTGCATATCCTAACTTGCCCATATTCTAAATCATCTG450.1495762007645006No Hit
CTGCGTTGGGGCAGGTTATCGAAATAACAATGTTAAGGAAACTCTTATCTCA440.14625228519195613No Hit
TTGTAGGGTAAGAACCTGGGCTTCCAGATGCTGCAGAAGATGGGCTGGAAGG440.14625228519195613No Hit
TGAAATGGGATCCCAAGCTGCATGTCAAAGAAATTCAATTTCTAATCCGTAA440.14625228519195613No Hit
TATGTTGGGGTGGCAGAGGACAGATATTAGAAATACTTTTTGGTTACAGAAA440.14625228519195613No Hit
TACAAAGGGATGTGTAGTTTGCTTTCTTCATTTTTTTTCTTTTAAAATGCTC430.14292836961941166No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTAT430.14292836961941166No Hit
TCAGCAGGGTGGGGGGTGTTGCCCCATCACTGCCCAGCTCCTCTGACTGCCC420.13960445404686722No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT420.13960445404686722No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTGTCTCTT410.13628053847432275No Hit
TATAAAGGGGGAACAGAATAGAGAACCCAGAAATAAAGTCAGATACTTACAG410.13628053847432275No Hit
AAGTTTGGGCATAGAAAAAGTATAAAGCTCAGTTAGTTTTTTTATTATTATT410.13628053847432275No Hit
ACTTATGGGGTATGGAAGCCACCGGGAGATTTCTGGATGGTGGTGCATCCGT400.1329566229017783No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTCTTATA400.1329566229017783No Hit
TATCATGGGATCTTTCTTTTTACTTCAAAACACCAGATCCTAGTTGACACTG400.1329566229017783No Hit
ATTTTTGGGATGAAAAGAGTATTTAACTAGCCTAAATGTAGGTAGAACTATA400.1329566229017783No Hit
CAATCAGGGAACTCTTCCTTGTAGGGCCCTTTCCTTATTCATTTAGGTAGTG400.1329566229017783No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTC390.12963270732923385No Hit
TCTTGGGGGAAACTGAATAGTTGGTTCCTGAAATCTTGGACTGTTTATGACC380.12630879175668938No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATG380.12630879175668938No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC370.12298487618414493No Hit
ATAATGGGGGGAGTGCAGTAGCACGATCTCGGCTCTCACCGCAACCTCCGTC370.12298487618414493No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGCCGTCT370.12298487618414493No Hit
TGCAAAGGGAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATT360.11966096061160046No Hit
GTCGCAGGGCTCTCCCCTCTGACCCCTAGAAGGGGAGTTGTAGCCCCATGAA350.11633704503905601No Hit
TATGGGGAACTGACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCA350.11633704503905601No Hit
TGCGTGAGGACAGACACAGCAACAACAAAAAAGGCAAAGAAAAAAGCAAGAC340.11301312946651156No Hit
GACCAAGGGGTCAGGGTCTCCTAATATTTATCTCAATTCTTTTATAAGTCTA330.10968921389396709No Hit
ACATGTGGGGAACTAGCGGAAAATTTATTAAATTAACTATTAACCACATTCA330.10968921389396709No Hit
GCAAATGGGATGGCATTGAGAATGAAGTCTTCCTGAGCCTTCCATGTATCCT330.10968921389396709No Hit
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCCCA330.10968921389396709No Hit
CTTAGTGGGCAGATCTTGCTTGTGGCAGTTACTACTGTGGTGTTCTAATGGT320.10636529832142264No Hit
ACGGCTGGGAACTTTGAGTCAGCTGGTAAATATGTATATCTTGTGTTAAAAA320.10636529832142264No Hit
CTTTCTGGGGAAGGAGAGGAGGAGGAAAGGAGAAGAGAAGGAGGAGGAGAAA320.10636529832142264No Hit
CTCATTGGGACAGGAACACTACAAGATGGGACTGTTTTTGATACTAATATTC320.10636529832142264No Hit
TTAATAGGGAAATGAAGACCAGAAAGTACAAATTGCTGGGAGGAAGAATAGG320.10636529832142264No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCG310.10304138274887817No Hit
ATCAGGGAGATGTAATAATTATATGTAAGGGCAAAATTTATTTTAGATATAA310.10304138274887817No Hit
TTGTACGGGATTCTATTTGGTATTATATTATTTGATGTTTGCTGTTCTTCAA310.10304138274887817No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)