FastQCFastQC Report
Sat 31 Dec 2022
SRR5849862.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849862.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81543
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC31073.8102596176250567No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCCGTCT28373.479145972063819No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGC5380.6597745974516513No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCTTATAC3400.41695792404007703No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA3180.3899782936610132No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTATACAC2640.3237555645487657No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCT2520.3090394025238218No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTA2240.27470169113228604No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGC2200.26979630379063807No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAT1900.23300589872827832No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT1640.20112088100756653No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC1450.17782029113473874No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCC1220.14961431392026295No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATG1050.12876641771825909No Hit
AGGTAGGGAACAAGAAGAATTAATTCAACAGACTTTAGAACATGGTGTTTTG1040.12754007088284708No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTCTTATA1010.12386103037661113No Hit
GAATCTGGGCTTCTATCGGGAGTACCTCATAGCCGCCTGCCCCCAAATATGT1000.12263468354119912No Hit
AACAGCGGGAACAAGGAAGACTTCATACCTTACAAGGGGGGCCTGTTCCATT930.11405025569331517No Hit
TTTCTGGGGAAATTATGTAGGAAGATTCCTGTAATCATGTTTTTTTAATTAT930.11405025569331517No Hit
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCCCA920.1128239088579032No Hit
TTTAAAGGGACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAAC860.10546582784543125No Hit
TCTCATGGGATTGAAAGACATGGGAATTTATTTATGACAACTGTTTTAGGTG850.10423948101001926No Hit
TGAACAGGAGATTCCAAAAAAAGTAATTCTAGTCCCTCAAGAGACAGAAAAC850.10423948101001926No Hit
CCACTAGGGATGGAATAGTCTTTCACCCTTTGAAATGCTCACATTATATTAA840.10301313417460728No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGC840.10301313417460728No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTC840.10301313417460728No Hit
ACTTAGAGGGATGAAACAAACAAAACCCCCTGGATATTTTATCTTTAGAAAG830.10178678733919527No Hit
AGCCTAGGGGCGATCTCAACAGGGGAGAAAAGAGAGATTTTTGGAAAGCACC820.10056044050378327No Hit
TAGGCTGGGCGCCTTTTCATCAATCGCCCACATCACTCGAGACGTAAATTAT820.10056044050378327No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)