FastQCFastQC Report
Sat 31 Dec 2022
SRR5849854.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849854.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49786
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC43098.655043586550436No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCCGTCT38337.698951512473386No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGC7161.4381553047041336No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA5781.1609689470935605No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGC3630.7291206363234645No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAT2510.5041577953641586No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCT2500.5021491985698792No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCTTATAC2360.4740288434499659No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCC2250.45193427871289116No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT2100.4218053267986984No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTA2060.4137709396215804No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGC2040.40975374603302134No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTATACAC1800.36154742297031295No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGCCGTCT1420.2852207447876913No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCC1080.21692845378218778No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGCCGTCT1020.20487687301651067No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC930.18679950186799502No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC810.16269634033664082No Hit
CTTGCGCGGGATTACAAGTTCAAAATCTTGAGGTAGTTTCTGTGATGGAATA770.15466195315952277No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATG710.14261037239384566No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTC690.13859317880528663No Hit
TGTCTCTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGA660.13256738842244808No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCG660.13256738842244808No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTGTCTCTTATACA620.12453300124533001No Hit
TAAGCAGGGAATGATGGGTTCTTTAGGGCTCTGATTCTTGTGTAAGAACAGT590.11850721086249147No Hit
CTGCAGGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA590.11850721086249147No Hit
TCATGCGGGGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCC590.11850721086249147No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAA590.11850721086249147No Hit
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCCCA590.11850721086249147No Hit
TATGTTGGGGGGCTGCTTACTTCACAGCAGCACAGCTGATGAAAGGGTGTCA570.11449001727393243No Hit
GACTCTGGGGGGAGCAATGCGTGGCCATCATGTCTGAGGAGGATGGAGATGA560.11248142047965291No Hit
CTAACAGGGATATTTTATCCATTCATGACATGCCTAATTTTTTCTAAATTTT560.11248142047965291No Hit
TTTTTTGGGGCTGGCATAGGGAAAATGTGTTAATCATTGTGAAAAGCACTTA560.11248142047965291No Hit
AAGAAGGGGCTCAGCAAGGGATCCAGTGTACCTGTCACTTAGACTTTGTGCA550.11047282368537341No Hit
CTTTGCGGGTACTACCACTGACATGACTTTCCAAAAAACACATAATTTGAAT540.10846422689109389No Hit
ATTTATGGGATAAATAAAGCTCCCATTCTTACCCTTTTTTTCTTTTTTCTTT540.10846422689109389No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCAGCTCGTATGCCGTCT540.10846422689109389No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTCTTATA530.10645563009681436No Hit
TCGCCAGGGATACAAGTTTTTGTGTGAATGTGTTTTCATTTCTCATGGGTAT530.10645563009681436No Hit
GTGCTTGGGGGGTGTATACCTTGCTTGCTTCCCTGATTCATTTTTTTGTTTT520.10444703330253484No Hit
GCTTATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC520.10444703330253484No Hit
TAATGCAGGGAAATGTGTGAGAGTGTTACTTACTGAAAACTGAAAGGATATG520.10444703330253484No Hit
CTTTGCGGGATCTACGAAAGGGGTGAGTTGATTTTCAGAATGAGTTATTGAT510.10243843650825533No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTAT500.10042983971397582No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)