FastQCFastQC Report
Sat 31 Dec 2022
SRR5849770.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849770.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74390
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGC20762.7906976744186047No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCCGTCT17752.386073396961957No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCA3500.47049334587982256No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAA2170.29170587444548995No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCTTATAC1970.2648205403952144No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCT1780.23927947304745262No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAACTCGTATGC1320.1774432047318188No Hit
GTCAATGGGATATGACCTTGAAAATCTTCCGGCATCCAAGGATTCCATTGTG1310.176098938029305No Hit
ATTACAGGGGATCTGTGATTGGGACTCCAGATGTAGAGCAGACTGAGCTCAT1230.16534480440919477No Hit
TACTCAGGGAAAAGAAAAAGAAAAAGAAAATGTTGGTAGCACTCATTAATCA1230.16534480440919477No Hit
ATTTTGGGGACTACAGTATACTTGGTTGATTTAGGTTGCACATTCACTGAAT1110.14921360397902944No Hit
GTAGTTGGGATAGAGAATAAATTAAAATCAGCAGATTGAGTTTTCAACATTG1100.14786933727651566No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCC1080.1451808038714881No Hit
CATCCAGGGTTGCATCACTTCTCATATTTTTCCCCCATCTCAGGGCCTATCT1050.14114800376394676No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAT1050.14114800376394676No Hit
CATGCAGGGACATTCATGTCATTGGCCCTTGTTCTGTCAACGTTGAATATTT1040.13980373706143298No Hit
GCTACAGGGTATGCATCAAGGTATTCTGGGGCATAAAAAAGCAAGTGTTCTC1030.13845947035891923No Hit
TCATAGGGACTGGTTAAGGTTTTTACATAACCGATATAAATGCGGAGACTTC1020.13711520365640545No Hit
TTTACTGGGAAGAATAAGAGTTTGGTGCTGACTTCTCTTTGAATGTTTGAAC1020.13711520365640545No Hit
GATACGGGGATCCTACTTGTTTTGCAAGGAGCAGTAGAACTACTCATTTAAA1000.13442667025137786No Hit
ACTCTGGGGGGTATATGGTTAGTTTATTTGTTTCATTTAAAAAATCTCTTTC970.13039387014383655No Hit
GGTGATGGGAGAGCAACCGATTAAAAATCTAAGCAAATATTTGAACATTTTA970.13039387014383655No Hit
CATCACAGGGAAGAAGGATGCAGAGGAGGACGACTCGCTGGCCAATTCCTCA940.1263610700362952No Hit
CAATTGGGGGCTAGTGGTGTGGGACAAAATATTCCTAATGAAAGGAAGTACC940.1263610700362952No Hit
GCAGTTGGGATACTCAAGGTTCCCAGGACTCTGCACTCTTGCAGCTCATTGA930.1250168033337814No Hit
CAGTAAGGGATTATTGGAGAAATAAAATTATTTTTATGAATTTACTGTAGCC920.12367253663126765No Hit
TTTGCTGGGAAGGCATGTACAGAAGTTCTAGTTTTTAAAGAAAAGCTAACAT890.11963973652372631No Hit
TGATGGGGATAGAAATGTAAAAATGTTTTAGTCTAGGGTTAAATAGTGTGAT880.11829546982121253No Hit
CGACTGGGGGATAGTGGAAAATAATGTAATACAATTTATAATGTTCAAAATA880.11829546982121253No Hit
TGATGAGGGAGGCATGAGCCACCGCGCTCTGCGAGGTACTTTTTCTATTTTT870.11695120311869873No Hit
TAGCCTGGGTGACAGAGTGAGAAGACAAAAGAAAGAAAGAAAAGAAGGAAGG870.11695120311869873No Hit
TGAAATGGGCAAGGTTAGAATTTACAGCTAACTGACTATATTCACAATCTTC870.11695120311869873No Hit
TCATTAGGGAAGGAACTGTAAGCTTTTATCTTTTAACCAACTGAACAATACA860.11560693641618498No Hit
AGATCAGGGATGTTATTACTGTTATTGACTAATTAACTTGGTTTAAATTCTA850.11426266971367119No Hit
GATTAAGGGGAGAAATCATTTTTGGATAATATGGATAAATTAGCTTTAAGAA850.11426266971367119No Hit
TAATCTGGGATCGGACCCTTTGAGAACAATAAGCATTTCATCTTGTCTTGGA840.11291840301115741No Hit
AAAAGCGGGGAACATGGTACAAAGGTAGACAGTCCAAGGGACCCTTTCTCTT840.11291840301115741No Hit
CGGCGAGGGGTATTTATCTTCTCATTATAAATATTTCTAAAATAAAAATAGA840.11291840301115741No Hit
TACGTCGGGATTCTTATGCAATTTTCTCATAAAATATTAAAAATTGTTTATC840.11291840301115741No Hit
TACGAAGGGAAGCGGTACCGCCAAAAGGGCAGGGAGGGCCACTCGCCACTCA840.11291840301115741No Hit
TCAAGGGGCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTA820.11022986960612985No Hit
CTACTAGGGAGAGGTATATATACCCAAGAGAATGCAAACATATGTCCACACA820.11022986960612985No Hit
TAAGTAGGGGAGGCTAACTTAGCTACTGTGAGTTTTTAAAAGACTATGAAAT810.10888560290361608No Hit
GCTACTGGGACTTATTTAAATCCCCAAACACCATCTTTAAAACTTGAGCCTA810.10888560290361608No Hit
GCTTATGGGGGAAAACTGTATGGTGGCTTATCAAAAAAATTGAACATAGAAT800.1075413362011023No Hit
AGTCACGGAGAAGTGATATAGTTTGGATATTTGTACTTACTCAGATGTCATG800.1075413362011023No Hit
GTTAGGGCAGATATGAAGACTATGATTACCGATACAGAAGACGATCACCTTC790.10619706949858852No Hit
TACCAAGGGGACCATGTGTTCTCATGTCTTTTTGCAGATTTAAAGGTTTTAT790.10619706949858852No Hit
TTTGTGGGGATATTTGATAATGAAAAAAATCTTTGCTTGTTTATTTCTGAAA790.10619706949858852No Hit
TTCGTAGGGGGGTGTCCAGAGATTTCTGTACACAGGGCGCCAGCCCAGGCCT780.10485280279607474No Hit
GTCATGGGGGTATCAATACATACTGATATGTAAATGTTTCTAAGGTGTTAGT780.10485280279607474No Hit
TTAGACGGGGAAAAAGGCTGGAAGTTGGACATAAGAAAGTGCCAGATGGAGC780.10485280279607474No Hit
CTTCGCGGGATACATACACATCAAACACAACAGCAGTTTTAGTGACCATAAT780.10485280279607474No Hit
TAAAAGGGGGGAAAAATGTGACAGACAAGCAGTTTTTCATTGCACACATATT770.10350853609356096No Hit
TCTAGCAGGGGTGGCTGATTCTTTTTCTTCTTTTACAGATTCTGTTTGTCAT770.10350853609356096No Hit
GGATTGGGGGAATTAAGTATTGAATTCCAGGAGCCTGTTCCTGCTTCGATAC770.10350853609356096No Hit
TCTACAGGGATTGATAATTTTTTAAAATAAAGAAATATTCACTGATTTTTTT760.10216426939104717No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)