FastQCFastQC Report
Sat 31 Dec 2022
SRR5849752.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849752.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86238
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGC24612.8537303740810316No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCCGTCT22132.5661541315893226No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCA4540.5264500568194995No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAA3210.37222570096709107No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCTTATAC3040.35251281337693363No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCT2170.25162921218024537No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAACTCGTATGC1820.21104385537698No Hit
AAGTGCGGGCAACAAGTGATTATTGATGATCTACAGTGTAACAGGCACGACG1450.16813933532781372No Hit
TGATATGGGAACTACCATAGCTAATATTCCTTTCCTTTCTGCAGGTCAATTT1370.15886268234421022No Hit
TGCGTTGGGCACTCTGCTCCACCAGCTTCTTGTGGAAGGCTGGGTATTATCC1360.15770310072125976No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAT1350.15654351909830932No Hit
GTTACTGGGGACAGCAAACTGCTGAGGTCACTTCCCCAGGCCCACTGAATGA1310.1519051926065076No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCC1300.15074561098355713No Hit
GATGGGGGAGACGCCAGAGGTGCAGCTCCAGCAGCAATGGCAGTGACGGCGT1260.1461072844917554No Hit
AACATTGGGACATAGGAATTTGAATGTGGGTCAGAATATCACTAATTTTGAT1240.1437881212458545No Hit
TAGCCTGGGCTTTGTACAACAGTACAATTATTATCTGAATTTTACAGATTTT1190.1379902131311023No Hit
GTTGACGGGACAGAACATGGTTGTTATTGTGCTGTTGACATGAATAGACCAT1170.13567104988520143No Hit
AAGCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTC1150.13335188663930053No Hit
CACCAGGGGCACAGGATGTGAGGAAAGAAAAGGAAATCAATTCTGACTGTGT1140.1321923050163501No Hit
TCATAGGGGTTAATCAAAATGCAATGCTTTGCAAGTCTTTACTGCTTGGAGG1140.1321923050163501No Hit
TTGGGTGGGAATCAATGTTTACTTTACATGTTAAAAATAAGAAACTAAGACC1130.13103272339339966No Hit
AATTTTGGGGTCCCCCATTTCACCTTATCCTTCTGTATCATACTCAGTAAAG1130.13103272339339966No Hit
TCGTCTGGGGCTGCCTTGTGTGTGCTGTACGTATCCTGCGTTAGGACCCTTG1120.12987314177044923No Hit
TTGTGTGGGAGAATTAACTACGGTTTGCATGACACTGAACAGCAGGAAAAGG1100.12755397852454833No Hit
TCCTAGGGATTTAAGTATTTGTTTATCTTTCTTTTAAAATATTTCTTTGAAT1100.12755397852454833No Hit
CTAACTGGGGAGGGAAGGATGCGTTCCAGGTATCTTTCCTGAGTTTATGAAA1100.12755397852454833No Hit
CCTGTTGGGACCTCAAGTGCAGGTGCAGGATGAAGAAATTACAGAGGATGAC1080.12523481527864747No Hit
AAATCAGGGGGAAGGTTTATGCTCTGAGCTCAGGATAGTTGGGGAACACATT1060.1229156520327466No Hit
AATAATGGGGGATGCAGTGCTCGCTGTTGCTGAATAGGCGATCACAACGTGC1060.1229156520327466No Hit
GCCTCAGCCTCCCGAGTGGCTGGGACTGCAGGCGCCCGCCACCAAGTCCGGT1060.1229156520327466No Hit
TATAGGGGGGCCAATAGGTCAGTTGCTGATAAAGAGGCCATTGCAAATTTCA1050.12175607040979615No Hit
GGCACTGGGGTCCTTAGGGACACACCACAAGCATGGAGGAATCAGAGTAGAG1050.12175607040979615No Hit
TAACGTTGGGGGTGAACATGACGGCAGCCGTGCGGACCTACCGCTGGCAGTG1050.12175607040979615No Hit
AGTTCAGGGAACCTCAAAAGTTTCAAAAGTTCTTTAACCAGATAAACTCCAT1050.12175607040979615No Hit
GATCAAGGGGGCTCTGCTGCTGCGGAGCCCCCGCCTGCCCGCCCGGGAGCTG1040.1205964887868457No Hit
GTTATAGGGATCTATATGTAAGTTACTAACATTATTATTTTGTCAGATGGAG1020.11827732554094482No Hit
GTACTTGGGAGGCTATCTTACCATGAAGGTTGTTTTGCTTATTGTATATTTG1010.1171177439179944No Hit
GCTCATGGGCAGGAGGAGAATGACTACTAATCTGGAAGAAAGGTCTTACTAT1010.1171177439179944No Hit
TATGCAGGGAAGACGCTATTGATAACGGAATGGTTGCAATTAAGGACATTTT1010.1171177439179944No Hit
TCAGTCAGGGATCCATATGCCACTTCTCTCCGTCTGTCCCACAGCGTAACTC1000.11595816229504395No Hit
GATCACGGGATAGGAGAAAAGTAGAAAATATAGATAAATGAAAAGAAGAAAA1000.11595816229504395No Hit
CAGAGGGGGATGACAGAAGAATTAAAAGAAATGAACAAAGTCATCCGGAAAT1000.11595816229504395No Hit
GATTATGGGACATTTTGATAGCCATTGATGTATGCATCTCTTGGCTGTACTA990.1147985806720935No Hit
GGCATTGGGATACCCAAGAGATCTGAAAATGTATGTCCACAAAAACTTGTTA980.11363899904914307No Hit
GGCAATGGGGATACTTTTTTATAGCTCTGCACGATATAGGATATTGGAAGTG980.11363899904914307No Hit
CTTAAAGGGTCTATGTGCGGGATTCCCAGATTCTTTCCTGAATAGATTTGAA980.11363899904914307No Hit
CAAGTAGGGAAATATATTAATTTTGAATTATGCCTTTGATAGCATACTTATA980.11363899904914307No Hit
AGCTTTGGGATATTTATGTAGAATCAAATTATATAAACAGTACCAATATCAT970.11247941742619262No Hit
GCTAGAGGGATCTCAAAACAGTTATAAGATTGACAATATGATCCCTACTTTG970.11247941742619262No Hit
TCTAGAGGGAGGGCTTGAACTCCTGGGCTCAAGCAATCTGTCTGCCTCGGCC970.11247941742619262No Hit
TGCAACGGGGACTGATAGAGTTCTGTCTCCTTTCTCTTTTGCTACCCAACCT970.11247941742619262No Hit
TAAGTCGGGGCATTTTGTTCAACTAAATTAATATTTGTAGATTGCAAGTTTT960.1113198358032422No Hit
GTAGTGGGGACTCTGCTATTGGATATATCCACAGTCTGATGGGCAACTTTGA950.11016025418029175No Hit
GCCAAGGGGCGATCTTTTGGAGGGGGCTCACTGCTTCCTTGAACTCCTGAGC950.11016025418029175No Hit
TAATTAGGGGTCCATTTAATTTCTTAAATGGAATCCTGAAATTTTGTGTCTA940.1090006725573413No Hit
GTAAGGGGGACATATTGTATTACATATGTAATACAATATACAATTACATATG940.1090006725573413No Hit
TAGCTTGGGGTGTAGTACACCTGCATGATCAAATGCTATAATTTACTATAAT920.10668150931144042No Hit
GAATGGGGAAAAGTTGAAAGCATTCCTCTTAAGAACTGGACTAAGACAAGGA920.10668150931144042No Hit
GTAACGGGGGGCTTGGGATTTATTAGGACACTTTTAGGGTAAAATTTATCTG920.10668150931144042No Hit
GCAGTGGGTATAAAGCACCAGACAGAAGAGTCTGGGTCATGTTATGCTACCA900.10436234606553955No Hit
CGCACTGGGGTGCAAGGAGCTTCTAAATTATCTAATATTTTTTCCTCTTTGC890.10320276444258912No Hit
CTTCAGGGGGAGAGTGAGGACTTTCCGCGCCCATTGGTGACCCTTCCAGGCA890.10320276444258912No Hit
GGAGGGTGGGGAGGCTGTGGTGAGAGACGGACCGAGACCGGAGATGTTTTCA890.10320276444258912No Hit
AAGACTGGGATATTCTAGATATGAACACTTGGCATTTGACATTTTCTAAAGC880.10204318281963867No Hit
CACCGTGGGATGGTATGTTTTTCTGTTTAGAAATTTTTATGTAGTCAGATTT870.10088360119668822No Hit
TAGCTGGGATTACAGGCGCTCTCCACCAAGCTCTGCTAATTTTTGTATTTTT870.10088360119668822No Hit
ATGTTCGGGACATTTTGTGCAGTGATTATTTTTTAAAGTCTTCTTTCATGTA870.10088360119668822No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)