FastQCFastQC Report
Sat 31 Dec 2022
SRR5849740.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849740.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48017
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCCGTCT13792.8718995355811483No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGC13622.8364954078763773No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCA3250.6768436178853323No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGT3040.6331091071912032No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAA2030.4227669367099152No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCTTATAC1860.387362809005144No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCT1500.3123893621009226No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGT1160.2415811066913801No Hit
GCAAATATTGTCCCACCCTGACAATATGCTTTTCTTCTCTGAAACCTTTCTT1120.2332507237020222No Hit
ACACGACGGGGTATTATTTTAATTGACTATTTTTCTGTTTAAACAAAATAAA1080.22492034071266426No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAACTCGTATGC1040.21658995772330633No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAT1020.21242476622862738No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCC980.20409438323926943No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCG950.197846595997251No Hit
TCGCGCTGGGAAGGCATCAGTTGCTGGCTTTTCTTTGCAATTTTATCCTCTG830.17285544702917716No Hit
CGGTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCT820.1707728512818377No Hit
AATTATGGGATTCTTACCGTAGTTCAGGCCCAAAACATGCCCATCTCATCTA810.16869025553449818No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATG740.15411208530312182No Hit
CCGAGAGGGGGGTCAAAGCTGTGGGTTATCAGAGTGTCATCTGGGAAATTAG730.15202948955578233No Hit
GTTATTGGGATGTTTCTCTGTCGGAGGATAAATAACAACGACAATTCCTGAA730.15202948955578233No Hit
CGATGAGGGATCTTGGACCGGGATTTGAATATAAGCTTTTTGAGGTCAGATA720.14994689380844284No Hit
GTGAACGGGAGTTCTACAAGGTCACATTTATGTGGAAGTCCAAAGTCAAGTC700.14578170231376386No Hit
GGCACGGGGGGTTGAGGCTGGCTGTCAGCCAGAATGCCTAAAGTCTGGCCAA650.13536872357706647No Hit
ATACGTGGGGAATGACACTGTATGGCAGACACACCTGAATGCATGTTCCAAG600.12495574484036903No Hit
TTGTGCGGGAGACTGCTTTCCACAGGGACTGAACTAATTTACAGTCCCATCA600.12495574484036903No Hit
ACATATGGGGATGTGGGCACCTTCTGCTTGGACTTTCCAGCCTCCAGAACTG580.12079055334569005No Hit
TGCTACGGGCTTTCATGTACTGAAGGGTCAAAGTTTCAATGTAGATCTTTAA570.11870795759835058No Hit
TCTTCAGGGGTTAGTAGAATTCTTGTGGAAAATAACATGGTTTAGTAAGTGA570.11870795759835058No Hit
ATGCGCGGGTAGGCCTAGGCTCATATGTGTGTTTGTGTCTTTGTTTTTAACA560.1166253618510111No Hit
CATTGAGGGTATAAGCTAAAATGTCCAATTTTATAATTGAGAATCAAAAAGA560.1166253618510111No Hit
TATGCCGGGATGTTTACTCAGGGTTAGAGCTGAGCCCTACCTAAGCTCAGAA540.11246017035633213No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTAT530.11037757460899265No Hit
GTAAATGGGAAGTAGTATATATGTTAGGATTACCCTCTGGTAATTGATACAG520.10829497886165317No Hit
TCTGTCGGGTGCTAATGCAGGAGCATGCTGATGCTGTCACCTAGAGAAACAA510.10621238311431369No Hit
ACGTTAGGGGAGTTCTGTATGCTTTCTGTAAAGTCTCTGAATTAGTTCCGAA510.10621238311431369No Hit
CCCTGATGAACCTAGATGCAAAAATCCTTAACAAAATACTAGCAAACCAAAC500.10412978736697419No Hit
GATAGAGGGTATACATTAAATGAAATGACATTAAGGATTCAGATTGAATTTT500.10412978736697419No Hit
TGTATGGGCTAATTCTTAATGCGCATAGGAATCAATATGAATATTTTCCTTA490.10204719161963471No Hit
TTTTTGGGGATGATAACGTGACCTATAAAGAAAGAGGAAAATTTGGCAGAGT490.10204719161963471No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)