FastQCFastQC Report
Sat 31 Dec 2022
SRR5849649.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849649.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110964
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCCGTCT12041.0850365884430988RNA PCR Primer, Index 27 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGC10930.9850041454886269No Hit
TTCGCTGGGATGTATGTATATGTGTATTTATTATTTTCTGAAGCTTTTGTGA2150.19375653365055334No Hit
ATTTAAGGGAACACACATCCTACATCTCAGGCCAGTTCATTCTTATCTAAAT2100.18925056775170326No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCTTATAC1930.173930283695613No Hit
CTATGAACGGGAGGTCACTTGGATATTAGATAATGAGGGAAAATGCCAGGAA1920.17302909051584298No Hit
AGACTGGGCTTTGATGACATTGTATAAGAAACAAAAAGCCAAAATGTTAGGT1860.16762193143722287No Hit
GTAACCGGGATAGTAAGCCCTAAATTTCAGTGAACGAATGTTCTTCAAGGAA1760.15860999963952271No Hit
GTACTGGGGAAATACGATAAAGTGATAATTTCTTCATCTCTCCATCTAAGGT1720.15500522692044266No Hit
TCACCCAGGGATACTTGATCCCAACAAAATGATTTACATTATAAATTCACAT1670.15049926102159258No Hit
GGGCATGGGGAAAAAACTTGTAGATAAACTATATAGCCTAGAAATAGTGACA1600.14419090876320248No Hit
GCGCACGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA1590.14328971558343245No Hit
CCGCCGGCTAAAGCCCCGGATCCTGGCCACCCAGATCCCCTCACCTGAAGGC1580.14238852240366245No Hit
TCGGTTGGGGGCAGTCTGTCTGAGGGCGGCCGAAGTGGCTGGCTCATTTAAG1570.14148732922389243No Hit
TCATCTGGGAGCAGTAAAAAGAAAAGTAGAAAATTACAGACAAGGAGAGCAA1540.13878374968458237No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCT1540.13878374968458237No Hit
TGTAGACGGGATAAAGACCCAATTACAGAATTTGTTGAATGTTTATATGTTA1530.13788255650481238No Hit
CACACTGGGGAGGCCCTGCATGGGCCAGCCCCTCCATCTGTACTTGGTCCAA1530.13788255650481238No Hit
GCATAAGGGATGTTTGGATCTCCGTAAACACATTTTTGTTCTTAGTCTATCT1530.13788255650481238No Hit
GGTCCTGGGAAGATCTAATTTTGTACTTTCCACTTCTCAGACTTGGAATTAA1520.13698136332504235No Hit
ATAAGCGGGCTTCATTACAGAAGGCATATCACCTTCTACTCCCTCTATAATA1520.13698136332504235No Hit
TTCAGGGGGAAAGGGGGAATTTGGAGTGGAAGGCATGAGGGAAAGGTACTCA1510.13608017014527235No Hit
CTATATGGGACTTGTTTTGTGGCTTGTCATGTGTAAGGCACTATGTTTAATC1510.13608017014527235No Hit
TACTGCGGGAGGCTGATTTTTAAAAATCTTTCCTAAGAAAAAAGCATGCATA1510.13608017014527235No Hit
TAAGAAGGGGGAGGCTTCTAAGCCAAATGGACAGAGGTATGATAGTGTCAAT1500.13517897696550232No Hit
CGCGTAGGGACCCAAGGGAGTGCCACGGCCCCTGACAGCCCCACAAGCTTTT1490.1342777837857323No Hit
GTATGCGGGATTCATAAGCTAATATTTTTTTAAAGTTACATTAAGATTTTTT1470.13247539742619227No Hit
ATATATGGGGCTTTGAGCCAGCCAACCAGATGGTGAAATGTGACCCTCGCCA1470.13247539742619227No Hit
CCAGAAGGGGGGGGCATGGCAGCATTGTAAAGTGTGGCTGGTACAAACCAAA1460.13157420424642227No Hit
TGTTTGGGGCATCTGTTTGAGGGAAAGGTCTCTGGGCAAGCAAGTGGTTATT1450.13067301106665224No Hit
GCTTGTGGGAGTGGAATGAACTGTTATGGTCTCCCTGATTGATTATAATTTT1450.13067301106665224No Hit
ATTGCTGGGATGTTGATTTTTGGGCAGGTATGTACTGGCAGTAAGTTGAAAA1440.12977181788688225No Hit
CGTGCTGGGATTACAGACGTGAGCCACCGTGCCCGGCCCTTAAATATTTATG1440.12977181788688225No Hit
CAGTAGGGGTCAAAAACAAGGTTAATTGATACCTACCATAAATTCTACCTGA1420.1279694315273422No Hit
ACGTCCGGGTGTCAGTAAGAACATAATTAAGTTATATTTTTCTCCAATTCAA1400.12616704516780217No Hit
GTAAGTGGGGTGTTCTGTCTTTAAAATGTAAGTGAATTTTGCATTCTCTATA1380.12436465880826214No Hit
TTGCTAGGGACACGAGGCTCAGCAGGCCATGGACATCCACTTCCACAGCCCG1370.12346346562849213No Hit
GGAATGGGGCAGCCTCAGCTCCAGGCTATCTGGCAATAACAAAGAAAGTGGC1370.12346346562849213No Hit
TCCAGGGGAACCTATGAATGAGACTTTTACACCACGTAAACTTGCTCTTCCT1360.12256227244872211No Hit
CTAAGCGTGGGCAATGCTAAAACTAATCGTCCCAACAATTATATTACTACCA1350.12166107926895209No Hit
CACCAGGGAAGGCAGAAGGATAAAGACAAGATTTAAAAAAATGAGAAAGAAG1350.12166107926895209No Hit
AGAATGGGGACAACAGTGTGGGAGAGAATCAACCGTAAAAATGTCTTCATTA1340.12075988608918208No Hit
GGGCATGGGCTGACCAAGCAAAGAAACATTTAGTTGATGGTGCTTGATATTT1340.12075988608918208No Hit
GCTATGGGGACCGAAATTTGATCAACAAGCTGTCCGTTGTGGATCAGAAGCT1330.11985869290941206No Hit
GACCTTGGGATTCATTTATCTGTTTATTCATTTATTTATTGTCTGTTCTGAG1300.11715511337010201No Hit
TCAAGTGGGATCTGCAAAAGGGGATCTATCCATGGGAGTCAATAGGTTACTA1300.11715511337010201No Hit
AGTTTAGGGCTGATAAAGTCCCTGGATGTAAGAAAGAAGATGAAATGTCTTT1300.11715511337010201No Hit
TAAATTGGGATTTAAAGTCACGCCTTGTGTTTTTTTTACTACTTTATTCAGC1290.116253920190332No Hit
GTTGTTGGGCAGCACACCTGCCAGCAACTTGGGGGAATAAGCGAAGGTTTCC1280.11535272701056198No Hit
GTCCTTGGGATACTTGAAAAAAATTTCATTTAGAAACCTAGTTCAGAATCTG1280.11535272701056198No Hit
GCTTTGGGGCTTCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGA1280.11535272701056198No Hit
TTCTAAGGGACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGC1270.11445153383079197No Hit
TGATACGGGATTTTTGTATTACTTAAGATAGATTATCAGAAAGACAGTTACT1250.11264914747125193No Hit
TAAATTGGGACACCCTGTTATGTTCTCCAGAAACCTAGCAGGTTGCTTTACA1250.11264914747125193No Hit
TTGTGGGAGGGCTAACTAGATAGTTACAGTAATTAAGGCAGTGTAGAGTTGT1250.11264914747125193No Hit
CGTAGGGGGGAACACTTTGGATTTTGTTGTAGTTTGATTCTAGGGTAGAACC1250.11264914747125193No Hit
ACAAGTGGGGTGGCTTTAACATCCATGTGACTAGTGTATATGGTATTTGCTC1240.11174795429148192No Hit
ACATGCGGGCACTTCCGCCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTG1240.11174795429148192No Hit
TCATAGGGGGCGATTTCATGATCGTGCAAACATAGAGAGTACTAACACAAAC1230.1108467611117119No Hit
TTAGCTGGGGTTGTGCCACAGAGTGTAGCCAGGTCCCCCTGCAGCTAGTCCT1230.1108467611117119No Hit
GCAACTGGGACTTCTTCTTCACTTTCACTGTTGGATTCTTCACAATCTACAA1220.10994556793194189No Hit
TTTGATGGGCCTGGCTGGTGGGAATACACAGAATATATACTGAAAGGAGGCT1220.10994556793194189No Hit
GTCTGGGGGGCAAAAGAATGATGTTGTTTCTTTGTATGTCCTTGCTTCTTTT1220.10994556793194189No Hit
GTTACTGGGACCTTGTGAGCAGTTGGTTACAGCGCTGTGTGATATCAAACAA1210.10904437475217188No Hit
GTTTCCCCAAAGTCCGAGCGTGCAGAATTTGCTAGAACTGTCAATCTGTCTT1210.10904437475217188No Hit
GCTGCTGGGGCGTGGGCAGCGGGCCCTGCGCCTCACTGCACCCCTCCCTGCA1210.10904437475217188No Hit
ATGTTGGGGGAATGGAGTCTGCACTGTGTATGGCAAAAAAATAGATGGACAG1200.10814318157240185No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCT1200.10814318157240185No Hit
TATGCTGGGAAGCCATATTGTTCATTTATCTCAGCATGTATATGACTGTTTA1200.10814318157240185No Hit
CAAAAAGGGACTCCATCATCATCTTAGATCTAGTGATGTATTTATATTGTTT1190.10724198839263184No Hit
GAACATGGGACAGAAATTAAGACTGTGGTACTAGCATCAAGATAAACAAATA1190.10724198839263184No Hit
TAAGCAGGGCTTGATTGACATGGGGAAGGGGGTTAGTAGACTATGTGGATTG1180.10634079521286183No Hit
GAATCAGGGATATGAGTAGAACTTTTATTTTAAAATGATTTTTAAAAATGCT1180.10634079521286183No Hit
GCGACAGGGAAAGCTTTTTAATTGAGATAATTCACATATCATAATATTCGCC1180.10634079521286183No Hit
GTATATGGGAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGA1180.10634079521286183No Hit
GCTTAAGGGCAAAAACACTCATATATTTCAAGAAAAAGAAATTATGTTAAAT1180.10634079521286183No Hit
GAAGAGGGGCATGTTGATCTATAATTACACCTATGGGATCAATAAGCATGTC1180.10634079521286183No Hit
TTGGCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1170.10543960203309181No Hit
ATCGCTGGGATACTTAAAGTTCCATCAACTGGACTTCAATGATTCTATGTCT1160.1045384088533218No Hit
CTCTAGTTGGGGTTGTAAATACTTTTGCTTTGAAAACTTTTTCATTGTCCTA1160.1045384088533218No Hit
TAACGAGGGACCATCATCTGTTTGGATCCTTTATTTTTTGTTGATTCTGAAC1150.10363721567355179No Hit
ATCGTAGGGGTTGTCTCCACTGTTTCCTGGAGAAAGGTCCAATTACCCAAAA1150.10363721567355179No Hit
GTTAAAGGGCTTGTGTCATATTTTAAAATTACTAACTGCCATCACCTGCTGG1140.10273602249378176No Hit
GGTAACGGGGAAATTGAATAATGGTAATTTTAAGCAGTATATCTATGAAATG1140.10273602249378176No Hit
CGTTAGGGGAAGGCTTTCAGACAACAGTAGGCTATTAATAGTTAAGTTTGAG1130.10183482931401175No Hit
TTCTAAGGGATAGTAGTGCATGCGAACAGTAACAGAAACCAGGAAGAATACA1130.10183482931401175No Hit
GTGCACGGGGAGCAGCCTGCTCCTTTTCAATGGCGAAACTGTCGGCATTAGC1130.10183482931401175No Hit
TGATGGGGTCTGTTGTTTGTGGCTGTTTCCACATTACAAAGGCTAAGCTGAG1120.10093363613424174No Hit
CATACGGGCAAACACCCCAGCAAGAAAACTCCCAAAGTGCAGAACCAGATTC1120.10093363613424174No Hit
CTCATTGGGATCTAAGAATTATTTTCATAGCCCACAGTCACAAAAATTTTTT1110.10003244295447172No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)