FastQCFastQC Report
Sat 31 Dec 2022
SRR5849609.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849609.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136292
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGC14521.0653596689460862No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCCGTCT14291.0484841369999707RNA PCR Primer, Index 27 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCTTATAC2000.14674375605317994No Hit
TCGACTGGGGTCACCATTTTGCATTATTTAGAATAGATTTTTAAATGAGCTA1910.14014028703078685No Hit
GCCTGGGGCACCACTCTACGTGGAATGAATCAGCAATGGACATTCCTGCAAA1840.13500425556892554No Hit
ATGTGGGAACATATTATTAACTATAGTCATGCTACAGTGGTATAGAACACTA1840.13500425556892554No Hit
CGCGAAGGGAGTTTAAAAAATGAGAGTTTTGAAAAGCCAGGGATGCTCTCGG1640.12032987996360754No Hit
CAGTTAGGAGATGAGATTCCTTAATTGGGAGGGATTTTGGACCACAGAATAA1590.11666128606227805No Hit
AGACATGGGGTGGGTTTACATTCTGGTGATAGGAGATAGGCAGTAAATTATT1570.11519384850174626No Hit
CAGCTGGGGGTTTAAGAAAAATATACTGAATTTATAGAAAGCAAGTATTCTC1550.11372641094121445No Hit
GCTTAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1550.11372641094121445No Hit
GCTCAGGGATCCTTGATGGTATTAAATGTTTTTGAATGAGAACTATAGAAGT1510.11079153582015085No Hit
TAGTCAGGGGGAGAGATTAGTGAAGGCTGGAGTAATCACAGATGGCTTCATG1510.11079153582015085No Hit
TAAGTGGGGGATTCTATGTGATTTTAAGACAGCCATCAGGGAGACTTGTAGG1480.10859037947935316No Hit
AATATAGGGATCCATTCATCTGTCAATTGACCTTTGGGTTGCTTCTGCCTTT1460.10712294191882135No Hit
TTCGAAGGGCTTGGTGAGACTGGAAAAAGAAGAAGAAAAGAATGGGGCTAGT1450.10638922313855545No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCT1410.10345434801749184No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCT1390.10198691045696007No Hit
CTGTGCGGGACTGCTACAGTGCACTGTATGGTGTGCTGATGAGAACAGACAA1390.10198691045696007No Hit
CTGTTCTGGGGGTACCTGAGCCCTGAGGGCCTCACGGCAGTGTCAGGAAGCC1380.10125319167669415No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)