FastQCFastQC Report
Sat 31 Dec 2022
SRR5849589.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849589.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45366
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGC8271.822951108759864No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCCGTCT8071.7788652294670015RNA PCR Primer, Index 27 (95% over 22bp)
AATCAAGGGACGTAGACATTTTTCCATAACCATCATGTCATTATCACACTCA1530.3372569765903981No Hit
GTAATAGGGAGGCGTGAGCCACCACGCCCGGCCGTTGTCTTTGATTTTAAGC1260.2777410395450337No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCT1250.2755367455803906No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCTTATAC1180.2601066878278887No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGT1180.2601066878278887No Hit
TATAATGGGATACCTAGATTGCCAAGAATTCTTTGGCCTTGAAATTCAGTAA1060.23365516025217123No Hit
AACTAGGGATGGATCACAGGGTATGAAAATGAGTAGATGAATGCTTAACAAA1010.22263369042895562No Hit
TCTATAGGGTGCAGGAACAGGAGAATGACATCTTCCTGGGCTGGGAAAAAGG900.19838645681788122No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCC850.1873649869946656No Hit
GATGCGGAGAGAGACATTAGGAAACTAACTGTACAAAAGAACCAAGTTTAAA840.1851606930300225No Hit
TGATTAGGGGGTCGGGGCCTTTGTGTATTAAGTAACTATTTGTTAACACAGG800.17634351717145No Hit
TATATAGGGGATACCCCAGGTAAGAAGCAAGATGCAGAACGGCATCAGTGTC800.17634351717145No Hit
GAGCCAGGGATAGTCAGAGAACCCAGCAATACAGATCCGTCCTTGGGCAAGG780.17193492924216372No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCT760.1675263413128775No Hit
TGTTGCGGGATACTAATATCAGAAAAATTCAATATCAGAGCAAAGAAAACTA750.16532204734823436No Hit
AATTCAGGGTAACTACTTTAGAGATTCAGTACAAAGTTCAGAGGAAAACAAA740.16311775338359122No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTA740.16311775338359122No Hit
GAGAGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG730.1609134594189481No Hit
AATCAGGGGAGATGTAAATGGAGCTAAGAGGGGTGCCTGACCAAGAGTAAAC660.14548340166644624No Hit
TTACTTGGGCGCTCTTCCGATCTGTATTTGGGACATAAAGTGTGATGGCAAC650.1432791077018031No Hit
GTTATAGGGAGCAAGAGCTATGATGCCGTTGTCTTCGATGTCTTGAAAGTGA650.1432791077018031No Hit
AGGTTAGGGACTGATAAAGCAGGATAAGTCTGAACTTAAATTTCTGACTTAG650.1432791077018031No Hit
CGATATGGGAAGGAGGACAGCAATAACCATTTACAGCAACTCACCAATTTTG620.13666622580787374No Hit
TCTTAAGGGGGACTCCTTGGTATTCCAATGGAGAAACAAATGATAATGGAAG610.1344619318432306No Hit
GCTTGGGGGCATCAGGAACCAAGCACTGTTCAAACTACCCTAATAAACTTTT610.1344619318432306No Hit
TTACTGCGGGACCTTCTTATAACTGATACTCTGCTTGGAACTCATTAAATCT590.13005334391394435No Hit
CGGCGCTGGGGTACGAAAAGGAGAATCTGGCCAGTCATGGCCAAGGTTAACA590.13005334391394435No Hit
TATCATGGGAGTTGGAAGTAAAGCACTCCTCAGCAAATGTAAAAGAACAGAA580.12784904994930124No Hit
GTGACAGGGGTTAGAGAAAATATTAGTGTATAGAGCCCCAAGTACTGTTGGA560.12344046202001499No Hit
TTATATGGGGATGGTCATGAACCCTCCAGTCCTGTAATTTGAATTCTAAAGA560.12344046202001499No Hit
TAGCTGGGGACAGACCATTTAACCACGGTACTCTACCTCCAGCTTGCTATTT550.12123616805537185No Hit
GCTCTGGGGGAGACATTTGGCTTGTACGGAGAAGAGAGACAGTGTGGTGGCA540.11903187409072874No Hit
ACAGTAGGGATGCATATACAATATCTACAAAAATGATTTAAGCATTTACTTT540.11903187409072874No Hit
GCATCGGGAGCAAAATGTGGAAGCAACCACAATGTCCTTCATTAGGTGAATA540.11903187409072874No Hit
CTACAACGGGAATATCATCTAGGTTTTCTTTAAAATTTTATTTTGTTCTTCA530.11682758012608561No Hit
CAACGGGGTTCACACCTAAAACAAAATGAAACCAAATTCTTACTGTAAATGA530.11682758012608561No Hit
CTAATTGGGATGTAAGCTTAACCAATTGAGATGTCTGTGGTGTTGGCTCTTG520.11462328616144249No Hit
TGGACACGGGTAAGTTGCCACCTCTGCATAAGAGCTCTCTGATCAGAAAGCA520.11462328616144249No Hit
ATGGTTGGGGACAATTTTTGTGATTGAATTATTATCCATGTTTGTTTTTCTG510.11241899219679938No Hit
CTAGCAGGGGGCTCATGTCGGCTAACGGAGCGGTGTGGGGCCGCGTGCGAAG510.11241899219679938No Hit
CATTAGGGAGAAGAAATCGGTTGACTGTACTACCTTTACTCTCAATCCAGGG510.11241899219679938No Hit
TTATAACGGGATAAAAAATTAGCCAGGTATGGTGGTGCATGTCTGTAATCCC510.11241899219679938No Hit
CGTTGGGGGAATTTCTTTGTTTATAGACAACATTAAGACATTATGTTCTTTA500.11021469823215624No Hit
ACTGTGGGGACCTGTATTTTCAAAATTTAAGCCTGACAAAAAAACCCCTTTA490.10801040426751311No Hit
TTAGGCGGGGTGTAAAATATTCTACTTCCTATAGCCACTAGCCACATGTAAC490.10801040426751311No Hit
GTTAACGGGATTTAAACATATCTCTGTAGAGTCAACAAAATAAAATAAACAT490.10801040426751311No Hit
CATAATGGGATAGTTCCAAAGCTAAATATATAAAAAAGGTATATTCTGAGAA480.10580611030286999No Hit
GGTGCGGGGGAGTGAGTCTGGCAGGCTTTTTAGTTCCTGAGTTAAATTTGTA480.10580611030286999No Hit
TACTAAGGGGATTATAATTAGCATAAATTATTTGCACCTTAATAAGTCCTCA480.10580611030286999No Hit
ATATATGGGAGAGTTCTGGAAGTGACCACGGACCAGGGTGGAGGAATAGACT480.10580611030286999No Hit
CACTGGTGGGGGGGCCGAGGTTCCGGCGCGGCTGCTGGCCGGCGGCTGAGCC480.10580611030286999No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATG480.10580611030286999No Hit
TTCAACGGGGGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGA480.10580611030286999No Hit
CGTACTGGGAGCCGTCTGCTTCACCGTCGTCATCTTCTCCATGCAGACCCGC480.10580611030286999No Hit
TATGCTGGGGTGTCCTTGGGCAAGTGAGTTAATTTCATTCTGTTTTTCTTTT480.10580611030286999No Hit
GCACCAGGGTCTTCCTTTAAACCAATAGTCCCAGAAGAGACCCTCTGAAATT480.10580611030286999No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGT480.10580611030286999No Hit
TGTAAGGGAGTTGAATTGAATGTCTATAAGGTCTAAAGGTAGAATGTGAATA480.10580611030286999No Hit
CGCTTCGGGACTTAAAATTTTACAACTTTATTGTCAGATAACAGATTAATTC470.10360181633822688No Hit
GCATAAGGGTACTGGGGCAGGGCAGCAGGCTTTTGTGTTCTCTAAAAATGTT470.10360181633822688No Hit
GAGCTGGGGAAGGTTGGGTCTTTCAGGCTGGTTTTGGTTTGTATTAGATCTG470.10360181633822688No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)