FastQCFastQC Report
Sat 31 Dec 2022
SRR5849408.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849408.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58353
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGC3680.6306445255599541No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGCCGTCT3260.5586687916645245No Hit
CAGCCTGGGTGATACAGCAAGACCCTGTCTCAAAACAAACAAACAAACAGAA1020.17479821088890032No Hit
GCAACCGGGGAATAATAAAGACTAAGGGGAAAAAACCCAACAACCTTTAGAT870.14909259164053262No Hit
AAAGAAGGGGACCAGGGGATTTTCCAAGGAGAGGTTGCTATGGTGACAGACT850.14566517574075025No Hit
CCGTCGGGCGAGGGGCCCGTGGTGGGATGCAGGAGCAGCCGGCATCCCCACG850.14566517574075025No Hit
TGTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA810.13881034394118552No Hit
CTCGCTGGGTTCCCAGGAGAACTGCTGATGCTGAGATGCTTGGATCCTACAA790.13538292804140317No Hit
GCTCAAGGGAATGCAGCAATATTAAAAAAGGTCATTCAGTGGTGCACCCACC780.133669220091512No Hit
TGTAAGGGGACATATTCCCTAATATACTATCTTGTTTTGTCTTACAAACACT770.13195551214162082No Hit
CAGTGCGGGGCTGGAGTTCTCTATTTACCCGGCGCCCCAGGTTTCCACAGCT760.13024180419172965No Hit
ATTGCAGGGAATGAAGCAGCAACTTCGAAAGGCGATGTTGGAAAAAGACGGA740.12681438829194727No Hit
TTGCGTGGGTCTGCTACAACTTCTGAGGCTAAGGGAACAAAGGAAAGAGGAG740.12681438829194727No Hit
CATGCGGGGATTCATAAATAGGAGAAGCACTGCCCCCTGAACTTGCAGAACT740.12681438829194727No Hit
GAAATTGGGCATTAATGTGAACATGCTTATTAAAAGTTAACCTCTTTATAAA740.12681438829194727No Hit
ATTTGGGATGGATTGTTTAACCTAAGCAAAAAATATTGATTTCCTTTTCCCT730.1251006803420561No Hit
CACACTGGGGCAGCTGGGATTACAGGCACACGCCACCACACCTGGCTAATTT720.12338697239216492No Hit
TTTCTAGGGGGGCCGGCTCCGACGTGCCCGACATCCGTCCCGACGCCAACCC700.11995955649238257No Hit
TAAGCCGGGATACCAAATGCCTGCTGCTACCACCCTTTTCAATTGCTATCTT700.11995955649238257No Hit
GTAGTTGGGATATAAGAAATGTATGCACTGTTTGAAATGTAAATTATTCTTA700.11995955649238257No Hit
TCATAGGGGAAGCTGAAATATATGCATGTAAAAACTTTGACATCTTTTTTTT700.11995955649238257No Hit
TTGAGCTGGGATCCATCAGAATGTCTAACTCTTCCTCGGTGCTTCCTTTGCA700.11995955649238257No Hit
TGCCATGGGTCTTTACTGGAGTGAGTGCGGGAGACAAACAGTTTACAACAAC690.1182458485424914No Hit
GGTCTGGGGGGAAAATACCTGATATAAATTCCCTCATCCTTTAAGACTCTGA690.1182458485424914No Hit
TACCGCGGGAAATAAAGAAAAATGAAGAAGCTGAGTCTCCAAGGGGTAAAAT690.1182458485424914No Hit
GCGGCCGGGCTAGGCACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTG680.1165321405926002No Hit
GACACTGGGAATGATGCAATTAGAAAGTGGTCCGGATTTGGGTGGCAATGGA680.1165321405926002No Hit
GATAAAGGGCATCCCTGTGTGCTGCAGCATCACCACGGCCTACTTCCCAATA680.1165321405926002No Hit
TGTGCTGGGGTAGACTTGCAAATAATTTAAACAAAATAAGAAAGAATGAAAA680.1165321405926002No Hit
AAAGCTGGGATTGTGGCAATTACTCTGCATAGCCTAGCAGTGCTGGTCTCTC680.1165321405926002No Hit
TGAGAAAGGGCAGTCGTTCTATCAGTTATTGAGAGATGGTTGCAAAATCTCC680.1165321405926002No Hit
CATGTCGGGCAGTAGTTCTGAGGTTGAGAAATTCTACCTATAAGCAAATGGA680.1165321405926002No Hit
GTACTTGGGAGATCTAATCATTTACTTTTGCCCAACAGCAGATAATGAAAAG680.1165321405926002No Hit
GCGTTCGGGCATGTGTCAGATCTGCATGCATTGCTTGCATTTTTCTGGTATC670.11481843264270902No Hit
ATTTCAGGGAGACTAAAAGGAGACGAAGGTGACATGGCACCTAAGTACAACA660.11310472469281785No Hit
ATCAGGGGACCTCTTAAAGAAAACCCACCCCGTAGATTCCCATCATACACTT660.11310472469281785No Hit
TTATTTGGGTCCTCAGAAAAATCCTCAAGGGTATAAGCATCCAAAGCAGTTC660.11310472469281785No Hit
ACGGGTGGGCTGCAGAAGAGAAACTCATGTCATGTTTATTACCTATATGGTT660.11310472469281785No Hit
GCTTGTGGGCGATCTGGACAGCGGGTCAATAAATATTTCATTAGTTTACATT660.11310472469281785No Hit
CGGATTGGGAGAATAGTACAAGGGTTAGGTGGGGTAGATTTAATGTTTCAAA650.11139101674292667No Hit
GCAAATGGGATGGAATTTGGCCTTCTAGATGCCTTGAGTACATTTTGATAAA650.11139101674292667No Hit
CTAGTAGGGGACTTCTCCAGCTGCTGGTATAAAAACAGTTGATCCTCTGCCT650.11139101674292667No Hit
TGAGTGGGGTTATGATTTCATGATTAGACAATGTGGAATTACATAACAGGCA640.1096773087930355No Hit
TCATAGGGGAATTGTATTAATAATGTCTTCTCCCATTTGTTTCAATAGGTTT640.1096773087930355No Hit
CAATATGGGAAGCATTCATTTTCTACCTGTGTGGAACTTTAATTAGTTTAAG640.1096773087930355No Hit
CACTCGGGGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT640.1096773087930355No Hit
TATGGTGGGGCCTCAGAAACACTTCCCATTTTAGCCTTTCAATGTGTGCACA640.1096773087930355No Hit
CTTTTTGGGTCCCATTGCTGTGAATGACCATGTTGGCCATGCTGTGAATGCA640.1096773087930355No Hit
TAAATGGGGCAATCCTAGTCTCTGATAAATCAGAGTTTAAACGAACAAAGAT630.10796360084314433No Hit
AATGGGGGGGCCTCCAAATCAGTATCACAAGCTAACTGTATTTGTTTAATTT630.10796360084314433No Hit
AAGCGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA630.10796360084314433No Hit
AGTTTGGGGGGCAAGAATGTATATAGAAGCAGGCAATCACAGAAAAGCTGAA630.10796360084314433No Hit
TGACTTGGGTAGGAGGGTGATCTAAGATTATTTCTTTCTGAAATAATGATAG630.10796360084314433No Hit
GTTATCGGGGACCGGAGGGAGGAGGGAGGATGCGCTGTGGGGTTGTTTTTGC630.10796360084314433No Hit
TTTACAGGGTGCTGAAAGAAATCACAGATGAAACAAACAAATGGAAACACAT620.10624989289325314No Hit
CATTGCGGGCGATCTACGTGCGGGAAGAAGAAAAAATTTTCTAACATTGTGA620.10624989289325314No Hit
CTTAGGGGTATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC620.10624989289325314No Hit
TTATCAGGGTCATAGCAATCGGAATTATTTTAAATTCAAAAATTGATGCCCT620.10624989289325314No Hit
TCTTGTGGGTTACAGTTAAAAATTATTTTCTACTATCACATTTGGGATTAAG620.10624989289325314No Hit
TACGATAGGGGTGGTGAACAGATTTCAACTGTGATGCAAATTCTTATTCATT620.10624989289325314No Hit
ACTGACGGGCCTCCTATTTATTCTAGCCACCTCTAGCCTAGCCGTTTACTCA620.10624989289325314No Hit
GCCTCCCGGGGTGCTGGGATTACAGGCGTGAGCCGCCACACCCAGTCCTTCC610.10453618494336195No Hit
GCAACTGGGAGCCATAAGCACATTTTTATAAATATTGCCAACAGTGATATAA610.10453618494336195No Hit
AACGGGGAGATGAAGAAGTTGATGAAATGATCAGGGAAGCAGATATTGATGG610.10453618494336195No Hit
GTTCATGGGCTTTGAAATCTTCGTGGAACAAAAGATATTTGTGGAACCAATC610.10453618494336195No Hit
TGCTATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA610.10453618494336195No Hit
TGAACTGGGAGGTTAAAGAACTAACATTTTCTGAACCCTTCATTAACTTTTT610.10453618494336195No Hit
GAATCTGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGT600.10282247699347077No Hit
GTCACAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC600.10282247699347077No Hit
GTAAACGGGACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTGGC600.10282247699347077No Hit
CACATTGGGTCTTCCGATCTATGACAGGGGCTAAACCTAGCCCCAAACCCAC590.10110876904357959No Hit
CAAGCTGGGAACTAGTTTTGACTTCTAAGTTTGGTACTGTGAGGCAAGTACT590.10110876904357959No Hit
TTCTCAGGGATTGGAAGGCTGAGGCGGGTAGATCACCTGAGGTCAGGAGTTT590.10110876904357959No Hit
GGTTCTGGGGTGGTAGCGACTTTCCTGCTATGCAGGAATCCCTCCCATGACG590.10110876904357959No Hit
TGTGACGGGCAATGATATGTATTGGGAGCCTTTTCAGTTGCTGACCCATAAG590.10110876904357959No Hit
TAATGTGGGATTGCATGGCATGACTGTGCTTCTGAACAACAGATGCCTTTTG590.10110876904357959No Hit
GCGTCAGGGCTCTTTCCCATCTTGCAAGATGGCGGGTGAAAAAGTTGAGAAG590.10110876904357959No Hit
GCGATAGGGGATCTTAGAATGGGTATTCTGGATATTAGCCCTTTGTTGGATG590.10110876904357959No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)