FastQCFastQC Report
Sat 31 Dec 2022
SRR5849330.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849330.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162508
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGTATGCCGTCT6770.4165948753292146No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGTATGC6730.4141334580451424No Hit
CGCTATGGGAGGGGGACATGTTTTCATTTCTCTTGGATGTATACCTAGAAGT1990.12245550988259041No Hit
ATAGCAGGGAAGAGAGGTAGGAATTATAACATTTTAAATTTCTGTGTTGGAA1890.11630196667240997No Hit
AGCAGTTGGCAATTTGTAAACAAGTACTTCATGGTGGTGAGGTAAGGAGAAG1760.10830236049917542No Hit
GACACCATTCTCTCTCCATTTACTTCTGGTGGTTACCCTGACTCTTGACTCT1730.1064562975361213No Hit
TAACACGGGATTTTCTTGGATGAAGAGGGATATCAAAAAAGCTTAAAAGCAA1680.1033795259310311No Hit
AACGACGGGGATCAAGTGAAAATTAAATTCTTTTGGGAAACATTTTCCTTCT1650.10153346296797695No Hit
GCTACGGGGGAAACATAAATTTTAGGTGGAAGTCAGTTTTAGAGAAGGAGTG1650.10153346296797695No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)