FastQCFastQC Report
Sat 31 Dec 2022
SRR5849059.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849059.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42409
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC16753.9496333325473367No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT15973.7657101087033413No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC3160.7451248555731095No Hit
GCCAAAGGGACCAGGACACCAAGGTGGACATCCCATCCCTCATTCAAACCAT2960.6979650545874696No Hit
TGCGCAGGGAACAAATGAGGACCTACAGGGCAGAAAGGTCTCTCTCCTCCTG2400.5659176118276781No Hit
CATATAGGGATCCGACACCAGCCGCCTCCACCATGCCGCCGAAGTTCGACCC2270.5352637411870121No Hit
CTTAGCGGGGAGGGTGGGATCTGGAAGCCAAGGGAGGAAAGAAGGATCACTG2270.5352637411870121No Hit
GACTCGGGATGCACATTCACGCTGTTTGTTTCATATATACAGGCATAAAATA2150.5069678605956283No Hit
ACGCGGGCGTGCAAAGGTAGCATAATCACTTGTTCCTTAAGTAGGGACCCGT2150.5069678605956283No Hit
TCGTTGGGGCGCTCGCGCCGAGGGGCTGCGAGAGTGACCGCGGCTGCTCCAG2100.4951779103492183No Hit
CTTTGTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC2060.4857459501520904No Hit
TAACGCCGGGATAGTGGGTGCCATTCTCAACATATTGGTTTGCTAACTTTAA2060.4857459501520904No Hit
AGTGTTGGGATATTTGACATTCTTCACTGTCTGTTGTTTACCTACCGTAGCT1920.4527340894621425No Hit
CCGTGCGGGGTCAGTGGTGGACCTGACCTGCCGTCTAGAAAAACCTGCCAAA1910.4503760994128605No Hit
GTAACTGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC1910.4503760994128605No Hit
AACCACAGGGGAGACATGGCGCTTGCCAGCGTGTTGGAGAGACCGCTACCGG1830.43151217901860456No Hit
CAGCTGGGGAAGAGGCGGGCATAACACAGCAAGACGAGAAGACCCTATGGAG1810.42679619892004056No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCA1800.4244382088707586No Hit
GGGTCTGGGCCGATCTAACGCCGGGGCACTGCGAGCAGTAGCCCAAACAATC1740.41029026857506656No Hit
AGCCATGGGAAGTGCAGTGAAAGAACTGCACGGTGAGTTTAAAACAACCGCT1710.4032162984272206No Hit
ATACGCGGGGTGTCAGTGAATGGGCAGCCATGCAGGTGGTGGAGACACGCGG1710.4032162984272206No Hit
AGTCTGGGTGGGAACACTGATGGGGCCTTGGACAGGGCTTTCTCTCAGGTAG1620.38199438798368274No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC1600.37727840788511874No Hit
TTATATGGGGGTCACGTGACGCGGTGCCTGGCGCCGAGCCTCCCAAGATGGC1580.37256242778655474No Hit
ATCAGCGGGCAAATTTGACCTGGTGTTATTTTAGCCCCAAATTTATGACATT1540.3631304675894268No Hit
GCACCAGGGACCTTTCACTTCATCTTGCCCTTCATTATTGCAGCCCTAGCAG1530.3607724775401448No Hit
TTATACGGGATCTTTACGCGGGGTAAAGTTCCTTACTTTGCTGATTTTTCAT1520.3584144874908628No Hit
GACTACGGGGACCTCATTCTTCATGTCATTAAGCAATAGGTTGCAGCAAACA1510.3560564974415808No Hit
TAGCCCAGGGATTTTGCAGTTGTTGAAATTGTCCCCGCAGTTTTCAATCATG1490.3513405173430168No Hit
CTTCACGGGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACT1450.34190855714588886No Hit
GCTAGCGGGATCTTACAAGAGCATGCATAATGTTTACAATGAAAACAAGCGG1440.33955056709660686No Hit
CTACGTGGGACTGCATTTTAGTTTTGAAAATGACTTCCCCACCACCTAGAAA1430.33719257704732486No Hit
TATTGCGGGAATCACCTCCCATTCCGATAAAATCACCTTCCACCCTTACTAC1410.33247659694876086No Hit
CTTTGCAGGGCGATCTCATTGCGGGATGGACTCTTCCGTCTCAATGCCTCCA1410.33247659694876086No Hit
GCACGAGGGACAAATATAATACATACTCATACAAAACTTTGGAAATTCAGTA1400.33011860689947886No Hit
GAGCAGGGAATCTATGTACATATATTGTCTGTCCATATGTATTTGTAAATAG1380.32540262680091486No Hit
GAAAACGGGATCTTAGGTTGGAGATGACAGAAATATGATTGATTTGAAGTGG1370.3230446367516329No Hit
GGTGTAGGGTGGACTGAACAGGCCTGATGGAGCTTGTGGCTGGCCTCAGATA1370.3230446367516329No Hit
GTATTTGGGAAGGTATGGTCGGAGGAGGAGCTCCTGCCTGCGGTTTTGTGTA1360.3206866467023509No Hit
TTGCGCGGGGTTTGAACAGAAAATTCAAACAAGACTCTTTCCAATTTAAAGG1340.3159706666037869No Hit
TGATTAGGGGGGAATCCTAACAGACTCTGCTTTCCTCTCTTCGTGGTTTGTC1290.304180716357377No Hit
ATTGTAGGGCAAAAGGTCCTGGAACAAGTTCTTCATCAGAACAATACAGTTT1280.301822726308095No Hit
TTAACTGGGATCCATGTCACCCACACACACCTGTATGCTGACAAACACGGAC1270.299464736258813No Hit
TAAGTCGGGGACCACCTCTTTTTTAATGTATTTTTAGTCCACTTACAGCTTT1240.292390766110967No Hit
TGTGGAGGGACCTCACAATATGGAAAGACGGGACAACCTATGGAACTATCTG1240.292390766110967No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCT1230.29003277606168504No Hit
CCCCTACCAGCAGATTCTGCGAAAGCACCAAATTTCTCAAGACCCTCTTCTC1230.29003277606168504No Hit
TAATAGGGGAACGAATCCCAGAACTTAAAGATTGGTTCTCTAAAATAAGACA1230.29003277606168504No Hit
AACCACGGCCACAGGCAAGGGGGTTGCAGTGGCCGCCTGCACCTTGGATGAG1210.28531679596312104No Hit
TAATACGGGTGGGGTTGGTACTGTAATTCTCTCTGTTTGACAGATAAGAAAA1200.28295880591383904No Hit
GCAAATGGGGGGACTGTAAATATGTATATTTAACTTTGTATAGCCCATGTAC1200.28295880591383904No Hit
GCTCGACGGGAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTT1190.28060081586455704No Hit
GAAGATGGCGAATTAGAAGATGGTGAAATAGACGATGCAGGATTTGAAGAAA1180.27824282581527504No Hit
TCTTTGGGGATATATCAAGATAGAAGATGAAAGAATGGGCTGCGTCTCATGG1170.2758848357659931No Hit
GTCTACGGGATGGATAATGTTGATTGGATAATGCACATCTCAGTTACAAGCA1160.2735268457167111No Hit
GTTCTAGGGATTGTAAAATATCTATTCCGTATTAGAAATAGCTAGTTGACAG1160.2735268457167111No Hit
AGAGTCGGGTTCCTCACGAACCTCAGGATTGAGCAAGGTCTTTGTAATTTTT1150.2711688556674291No Hit
GTCATGGGACCATAACTGTTAAAACAATTTTTAGCTTTCCTCAAGTTAGTTA1110.2617368954703011No Hit
ACACTAGGGATTACCCACATAGGATGAATAATAGCAGTTCTACCGTACAACC1110.2617368954703011No Hit
ATATATGGGATCATGAGCCTCCTCAACAAGCCCAAGAGTGAGATGACCCCAG1090.2570209153717371No Hit
GGCTATGGGAGATTGTATACAGGCATTTCACATTATTAGGACATGGAATGTT1050.24758895517460916No Hit
CAATGTGGGGATTAATACTGGGTATGTTCTGGTAAATCCATCCTTATTGTAT1040.24523096512532716No Hit
CCCCCTCTACCTAAAACTCACAGCCCTCGCTGTCACTTTCCTAGGACTTCTA1040.24523096512532716No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCAT1030.2428729750760452No Hit
CCGATTGGGACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTCTGAGCCTTTT1030.2428729750760452No Hit
AGCAAAGGGGTGTCACCTGGATTTTTTTCTGCCCATGAATGTTGCCAGTCAG1010.2381569949774812No Hit
TCATTGGGGGTGCTATCAACAGATGGCTATTGAACACTTGAAATGTGGCTAG960.22636704473107125No Hit
TCTGAAGGGATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACC940.22165106463250725No Hit
GTGTAAGGGTAAATCAAGGTCATTTATAATCATTTTCCTTTTTTATTTAAGA920.21693508453394325No Hit
TGTCTAGGGGATCTAGGAACGGGGGTGTGTCTCAGCTAATCCAAAGGGAGTT880.2075031243368153No Hit
TGAAGGGATAACATAGCATGTTAGAGTTCTCTAGAGGGACAGAACTAACAGG880.2075031243368153No Hit
CTCACTGGGGGACCCCAACTCTCCTGAGCGCGTGACCTTTGTGTCCAGAGCC880.2075031243368153No Hit
TAACGGAGGGGCAGATAATTTTTAGCTTAGGGCCTGGTGGCTAGGTCGGTTC870.20514513428753328No Hit
AAGTAGGGGAGGCTAGTGTAACTCGCATCCCATTGCAGTGCCGTTTCTTGAC850.20042915418896934No Hit
ACGTAGGGGGGAAGGTGAATGGAAGAGGGCACGTAGGGTGCTTCAAAGATAA850.20042915418896934No Hit
ATGCAAGGGGGGGCGGCAGAGCTCGGCGGCTGGGACTGGAGGACAGCGGTGG850.20042915418896934No Hit
GACATGGGGGTGAGGCCAAATATCATTCTGAGGGGCCACAGTAATTACAAAC850.20042915418896934No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCAACTCGTATGC840.1980711641396873No Hit
GTAAACGGGGAGCTAAGTCAGCCAACACCTCTTGTTGCTCAGAGCTTCCATG830.19571317409040534No Hit
ATAAATGGGATTATATAGTTGTTTACATACTTATAAGTCTATCATTTAAAGA820.19335518404112334No Hit
ATTTTAGGGACTATAGAATGCAGTGAAAAAGGCATTGATGGTAACATGCATG810.19099719399184137No Hit
GCAAAAGGGATGCAAAAGGAATCGTGCTGGAAAAAGTAGGAGTTGAAGCCAA800.18863920394255937No Hit
CGAGCAGGGTACCCTGTGTCACCCACAGCTCAGTGGCCTGCCTGCCGGCCCT790.18628121389327737No Hit
TTGAGGGGATTCCCTATCTTTTTTAATATTAATAAAGTAAAAAAGATTCATA760.1792072437454314No Hit
CACTTTGGGACATTAATCCAGGTTCTGCCACTGGGGCATATAATGCCTTGGA760.1792072437454314No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCC720.16977528354830343No Hit
TACAGAGGGGTGGCCTGACTTGAGGGAACGATGTGTCTGTATTTCATGTGGC700.16505930344973943No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCACCTCGTATGC690.16270131340045743No Hit
CTCTCAGGGCTAGGAGCTTTCCAATACTCATTTAATTAAGATTTAATCATTT690.16270131340045743No Hit
CTTATGGGGAACTATTTATATTATCCTAACTACTACCGCATTCCTACTACTC690.16270131340045743No Hit
AAGACAGGGAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCACTCCTAAAC680.16034332335117546No Hit
GCTTCCGGGATAGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCTTTG680.16034332335117546No Hit
ATTTTTGGGAGAGGCATTGACAACAGGGTTCGTAGAAGATTCAAGGGCCAGA680.16034332335117546No Hit
TGAACAGGGGACTCTGATCCCATCACTGTGACGGTCCCTGTAGAAGTCTCCA680.16034332335117546No Hit
TACGAAGGGATGGAATGTAAGAAGATGAAGGTGACTGGGATTCAGCGAGAGA640.1509113631540475No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGT630.1485533731047655No Hit
CAGTATGGACAGTGTAACAACAAAACCAAAATGGCTGGACAGACTTCTTGTG620.1461953830554835No Hit
GCGTTGGGGAATCCCAGCACTTTGGGAGGCTGAGGCGGGCAGATCACCTGAG620.1461953830554835No Hit
GTTCACGGGCTTCTCTCTAAGGAGAATGGCCCAGTCCTCTCCCAAGTCCACA610.14383739300620152No Hit
CGAACGGGAGGTGCCCACCATCACGCCTGGCTAATTTTTGTATTTTTAGTAC610.14383739300620152No Hit
GCCATCGGGATTTGAGTACATGTGAATCATGGATTTGGTATTCAACTTTTTC600.14147940295691952No Hit
CGGGCCGGGCGATCTGAGTCTGGGAGATGGTGAAATGTGACCCTCGCCATGG580.13676342285835555No Hit
TAAGTGGGGGTGTGGGGCAGCGCCTCGTACGCCGTGAGCCTGGCGGCGCTGC570.13440543280907355No Hit
TGCCCAAGAAAAGAACTCTGCTGTCCAGGCACTGCTTGGCTTACTATCCCAG570.13440543280907355No Hit
GTAATGGGGGCTTCTGGATATCAGGAGCTACCTGGAAAAAGTCGCCACAGGC560.13204744275979155No Hit
CCGGGGGCTCGCTCTGTCTATGCAGGGGCCCCAAAGCCCGCACTTTGTCCGT560.13204744275979155No Hit
TATTCCGGGAGGTTCTCTTTTACCTCTTCTTTATTAAATTCCTTGTAATTTG510.1202574925133816No Hit
CTACCCCGGGCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCA510.1202574925133816No Hit
GTAGCTGGGGTTTTGATATTAGGAGCCAGCCCATGTAATGCCTCTCAGGCTT510.1202574925133816No Hit
ACTCATGGGACTTTGGGAGGCCGAGGTAGGCGGATCATGAGGTCAGGAGATC500.1178995024640996No Hit
TATTCCGGGACCCACAATCTGAGCACGCTACCAAATCTCAAAATATCCTAAG500.1178995024640996No Hit
TCTTAAGGGGGGAACCTGAGGGATCGGAATCCTGGTCCACTCCGCAGAGGCC500.1178995024640996No Hit
ATAATCGGGGCCTCATTATCATTCTTGAGTCAGATACTTCTGTTGATGGGAG500.1178995024640996No Hit
TTTATGGGGGATCTCGGCTCACTGCAACCTCCGCCTCCCAGGTTCTCAAACA490.11554151241481761No Hit
CTTCTAGGGGACCGCCAGATTCTCCCTTAAGGAATTGACTTCAGCAGGGGTG470.11082553231625362No Hit
TGTCTTGGGATCGGGCGAGGCCTATATTACGGATCATTTCTCTACTCAGAAA470.11082553231625362No Hit
AGCACCGGGATTTCCATTTTAATGGTTCTTTTATACTCTAAAATATTGACTT460.10846754226697163No Hit
TTTCACGGGGGCCCTTCAAAACAATGATCTTCAACAGTAAATGCAAGCACAA450.10610955221768965No Hit
ACACGGGGGAGGCCAGGCGGGGTGGCTCATGCTTCTAATCCCAGCACTTTGA450.10610955221768965No Hit
GTAACAGGGAGCTCAGTTTCGGGCCTCCCTGTCGGGGGAGTTTTCTTGTCTG450.10610955221768965No Hit
CTAGCTGGGCCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.10610955221768965No Hit
TGGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG450.10610955221768965No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCACCTCGTATGCCGTCT440.10375156216840765No Hit
CTCTTAGGGATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATATTGTAA440.10375156216840765No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)