FastQCFastQC Report
Sat 31 Dec 2022
SRR5849049.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849049.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166530
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT29771.7876658860265418No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC22401.3451029844472466No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC3360.201765447667087No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGT2520.15132408575031525No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC2050.12310094277307392No Hit
AAGCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTC1800.10808863267879662No Hit
ATGGTCGGGGGCCCTGGTGGATGGGAGCTCAGAAGGGGTGTGATGACAGGGC1790.10748814027502553No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)