FastQCFastQC Report
Sat 31 Dec 2022
SRR5848598.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848598.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181829
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGTGCTTATCTCGTATGCCGTCT8290.45592287258908093TruSeq Adapter, Index 10 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGTGCTTATCTCGTATGC7580.41687519592584243No Hit
TATAGCGGGATTCAGACTTCGAAAAAATAGTTTGTTGTTGCTTGTTAAATTT2550.14024165562149052No Hit
CTTGCGCCGGGAAGTCACAAAAGTAAGCTGTGACATTGTCTATAAGCATTTG2420.13309208102117923No Hit
ATATCGGGGATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTA2400.1319921464672852No Hit
CACAAGAGGAAAAAAAGCAGCAAGACAGAGATTTGTTGGTGCCAACTAAATG2350.1292423100825501No Hit
TATGAGGGGGAGAGAGATCCTTCTTTTAAAGTGATTTTATCAAAGCCCCTTG2330.12814237552865604No Hit
AAAGCAGGGTGTGGAGGAAGGGAAATATCCGTCATCCAAAGATTCTATATCT2270.12484257186697392No Hit
GTCGGGGATAATCACTTGTTCCTTAAGTAGGGACCCGTATGAATGGCTCCAC2220.12209273548223881No Hit
GAACTAGGGTATTAAATCTTGAAATCAAGTAGTGTTAATCTTGTAACTTTGT2150.11824296454360965No Hit
CTACAACGGGGATTCAGAATTTGATGAAGTAGATTGGTTGGAGTAAGAAAAA2150.11824296454360965No Hit
ATACCAGGGGTCACAGAACCTGATTTATTTTGGAGGTAGAGAAAAAAGAGGA2150.11824296454360965No Hit
TTCGTTGGGCAGTAGGAGGACTAGGCTCCATTATAAGAGTTCTTACAGCAAG2140.11769299726666263No Hit
TCACCTGGGTCATAAGGATTAAAAAAATGCATCTTTAAATTCCACTTGAGGT2140.11769299726666263No Hit
ATGTTAGGGAGGGGTTGCAATCCTGGTCTCTGATAAAACAGACTTTAAACCA2120.11659306271276859No Hit
CACTCCGGGATTCAAACCTTTTGCATGTTAAAAAAAATTTTCTTACTGATTT2110.11604309543582157No Hit
GATTCCGGGGTAGGCACTGTGATCATGATGAAAATGAATGAGGATGGCCTGA2110.11604309543582157No Hit
TCAGTGGGCAATTGGCAAGGGATTGATTCTTCAATGGGTTCAGCAATTCACT2090.11494316088192752No Hit
CGCGTAGGGAGCATGGGGGCCCAGTGCTTTTACAACAGAGGAATGCTAAGGG2080.11439319360498051No Hit
GCCTGCGGGCTCCCAAATGGTAAGTGGTGCATAGGTAGTAAATGTTTGAGTT2080.11439319360498051No Hit
GTAATAGGGAATTCCTTGTTGTTCATAAATTAAGCATCACTAAAGTCTCTTG2060.11329325905108645No Hit
CACAGGGACTCCTAAAACTGCTATACTGTGAACATATTGCTTGTCTCATAAC2060.11329325905108645No Hit
ACATGCTAGGGCTATGAGGCATAATTATAACAAGCTCCATCTGCCTACGACA2040.11219332449719241No Hit
GTCTAAGGGGCTCACTGCAACATCCACCTCCTGGGTTCGAGCAATTCTCCTG2040.11219332449719241No Hit
TCTTTTGGGAGGGGGCACAATGGCAAACAGGATATCCCACACTTCTTAACAT2040.11219332449719241No Hit
TTACTAGGGGTTGTTCAGGGAGAAACTGACACCACTCAACTGCCTGGCACTG2040.11219332449719241No Hit
CACCGCGGGATGTGCAGAGAGACTAAAGTCCGAACTGATCTTCATTTCAGTC2020.11109338994329837No Hit
GTATGGGGGTGGTCTGCTGCACCTATGAACCGGTCATCTAGGTTTTAAGCCC2020.11109338994329837No Hit
GCACTGCAGACGGGAATGCCTAGGTCCCTTCCCGACCCAGCCAGCTGCAGGG2020.11109338994329837No Hit
GTTGCTGGGACACGACGTTCTTCCGTTCTAACTGAGGGATACCAACATTCAG2000.10999345538940433No Hit
GACTATGGGATGATTTGGTATCCTAGAAACAGATAAATTTGTCTAAAGTATA1990.10944348811245731No Hit
TTTACAGGGAGAACTTTGACTATTTAAAAGTTTCATTTTCTTTTGCAATTTT1980.10889352083551028No Hit
GACCTAGGGATCGATCAGATGTTTATTTGGTCTGAAAAATGGATTCAATACT1970.10834355355856327No Hit
AAAGCAGGGGGATTGTTGTCCCAACCCTGTACTGTACTCCCAGTGGCCAAAC1970.10834355355856327No Hit
GCGAGGGGTGGTCCCTGGGAGCCAGCTGGAGACAGGTGGTCCTGTAAAAGCT1960.10779358628161624No Hit
ACAACCGGCACGACAGCCCCGAAGATGTGAAGCGACGAGCCATGGCCGACCC1960.10779358628161624No Hit
AAAGCTGGGAAGGAAGGTGAAATAAAACTATTTTCAGGAAATAAAAATTAAG1950.10724361900466924No Hit
ATCCGTGGGCGGCAAAGAATTTCTCGGCCCACACTGCAAATAGTGCTGAAGT1940.1066936517277222No Hit
GCAGTGGGACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCAT1940.1066936517277222No Hit
ACCAGCGGGGCTCAAAAAAGTAACTTACTCAAGATCACAAAACAATTAAGTT1940.1066936517277222No Hit
GTTCGTGGGGAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTCAGA1930.10614368445077517No Hit
GCTCAAGGGATGTGGAGTAGGCTACATGGAATACCGTTTGAGTTTGTGTAAG1930.10614368445077517No Hit
ACGTAAGGGTCTGTGTGAGGGAGTAACACTCCTGATACAACTCTGGTTATAA1930.10614368445077517No Hit
TTTACCGGGAGGCACGGGGCTTTTCACTTTATGCCTTCTTGTTACTCTTGAA1930.10614368445077517No Hit
GTGCATGGGACCAGCGGCTAAGTCTTTTTTCTAGTTCGCAATGTTTTGTTTT1920.10559371717382816No Hit
TTTAAGGGGATGTAACAATGACAAAAATTATAAAAAGACATGGCTGGGCCCC1920.10559371717382816No Hit
CGCGCAGGGGGGCTCTGATTAAAAAAGGAAAACTTCTAATATATTGCTTGGA1920.10559371717382816No Hit
TTTGCTGGGCTCTTAATGCCTGGCTTAAATGAAAACTGCTGGAGTCCCATAT1920.10559371717382816No Hit
TGCGCAGGGAGTACTGGGATTACAGGCATGAGCCACCGCGCCCAGCCTGTGC1920.10559371717382816No Hit
GCTAGGGGGAAAGCGTTTATTGTAAGTGTAACACAGAGATACTATCAGGAGT1900.10449378261993411No Hit
CGCTTTGGGTACATTTGGTCTCAGAATTTAGGTTCCTGCCCTGTTGGTTTTT1900.10449378261993411No Hit
CAATATGGGATCTTGCAAATTGCCATTCAGTAGAATATCTCTCTGGACCTTG1900.10449378261993411No Hit
TGCCTTGGGTATGCCAGTAACTTTGCTTATCTGTCCAATTAGTCTGGCAAGC1900.10449378261993411No Hit
TAAGCTGGGACGTTAATGTACTTTTTTTTAGGATTTAAAAAAAGCTGTGTTT1890.10394381534298709No Hit
CGCTATGGGTAGTAAGTGAGTTCTCACGAGATCTGATGGTTTTATGGGGGGC1890.10394381534298709No Hit
TAGCCATGGGATTATTGAGGCTTTCACATATCAGTGATTTTAAAATATCAGT1890.10394381534298709No Hit
GGAGATGGGTCTTCCGATCTTTGTGAGGGTTGCCCTGGAAATGTTTGCTTAC1880.10339384806604007No Hit
CTTGCAGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCAA1880.10339384806604007No Hit
TGCATCGGGAAGCCTGAGGCTGCAGCCTTTTATTTATGTTTTCCCTTTGGCT1870.10284388078909305No Hit
GTTTTTGGGAAAGACTAAAGATTATGGAATATTATGAGAAGAAAGAGAAACA1870.10284388078909305No Hit
TAGCTAGGGCAACTCTGAATTGTAGCAGTGATGTCTTATATGCTTACGAGTT1860.10229391351214603No Hit
CGTGACGGGGTATGACTGAGGTATCCTGCGGTGGCTGGCGTGCTGGGCATGG1860.10229391351214603No Hit
GGTCTAGGGGTTTTCACAGATAATTTTCTAGCGTATAGGCTATTTGCCTTTT1850.101743946235199No Hit
AACTAAGGGCTAGAATGGGCAAGAAGGATTCTCCTCATTCCTCTCTTGACTT1850.101743946235199No Hit
TTTAATGGGACCCCTCCACTCTATTTTTGTGTTGTTTTGCACAGACTCGGGA1850.101743946235199No Hit
AAATGGTGGGGTTTGGGAGATAGGATGAAGTCATGATTTTGCTTTTGCAAGT1830.10064401168130496No Hit
ATAAGCGGGATGTGATGGGCTGTGCAGAAGGGGGCTGTATCAACATCAATTA1830.10064401168130496No Hit
TGCAATGGGGACTTCTGAGTTCTTTAACTTTGACAGAATCTCCATTGTTTCA1830.10064401168130496No Hit
AACAGGGAGGAGCAGTGTGACTTCTTGCCACAAGGCAGAACCAGGGCAACTA1820.10009404440435793No Hit
ATTGATGGGGTTGTTGGTAACATTAAATTAGGGAACCAAAGAAAGAGGAGAG1820.10009404440435793No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)