FastQCFastQC Report
Sat 31 Dec 2022
SRR5848580.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848580.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92023
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCTCGTATGC9681.051910935309651No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCTCGTATGCCGTCT8620.936722341153842Illumina PCR Primer Index 12 (95% over 21bp)
TTTAAAAGGGAGGATTCGAGAAGATATTGCAAAATATTTGAGGAATTTAGAT2070.22494376405898525No Hit
CTGCACGGGATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGT2000.21733697010529973No Hit
ACTGCAGGGATGCGTACCAGGGGATGGATTTAAGCATTTGTGTGTAATAGGA1840.1999500124968758No Hit
CAAAGGGGGCAAAGAACAAGTCATTAGAGAAATTAAAAATTATTTTGAACTA1840.1999500124968758No Hit
TAACTTGGGATGGTGGTGCAGTTCTTACGAAACTTTGGTAAAGTTTTGGGCT1740.1890831639916108No Hit
GTTAATGGGATATTTAAACGGCTGAAGGAAAAAAACTTTTACCCAGGAATAG1730.1879964791410843No Hit
TCAATGGGGATTGGAAAGCCAGAAGTTGAATATGATTGTGATAATTTGCAAC1720.1869097942905578No Hit
CGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAG1650.1793030003368723No Hit
GGTAGCTGGGGTTTTTACAATGGATTCTTAGATATGACACCAAAGTACAAGC1640.1782163154863458No Hit
CAATGCGGGTGTGTGGCTCAGAGGTGAAGCGTTGGAAGGGTTTGTGGGAGAT1530.16626278213055432No Hit
ACCATGGGGGATCTCATAGAGGGAAGGATGTTGTGGAACAAATGGAAAAATG1510.1640894124295013No Hit
TACGACAGGGTCTTCCGATCTTGCTTGGGGAGCAGAAAGAAGAAAATAACAA1500.16300272757897483No Hit
CAACCAGGGACCTATTAGAAATGCACTTTACCTGTAAGGACACAAGTAAACT1490.16191604272844834No Hit
ACGGGTGGGCATGACAACTGAAATATCTTTCAAGGAAGTGATAGAAGCAATT1470.1597426730273953No Hit
GAATTAGGGTGAGTGTCTTAATATTTCTCAAAATTAATTTTTATATATTTAT1460.15865598817686882No Hit
TATAGGGGACACATCAACAGGAAGGTAAGACAGCATTGCTGGGCCAAAAGTC1450.15756930332634234No Hit
CGCACAGGGACTGTGTCTGACTCTCAACAAATAATGACAAGAAATGAAAGGC1450.15756930332634234No Hit
TAACTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1440.15648261847581582No Hit
GGTATGGGGGGGCGGGGGCCGGCAGGTGCTCCGCAGCCGTCTGTGCCACCCA1410.15322256392423633No Hit
TGGTTCGGGGGAAAAAGCCTTTTAGATGAAAGTCAATCTTCGGTTTTGTTGT1410.15322256392423633No Hit
AAATATGGGAGGATCAAATCTTAATATAACTATAATCAGTTTTCTCTTTATA1410.15322256392423633No Hit
GCGTTCGGGTGTTTGCAGACAACATGATTCTATATTTAGAAAACACCATCAT1380.14996250937265682No Hit
TTGACTCGGGCGGTCGAGTCACCCCCAACCTCATGGGCCACCTCTGTGGAAA1370.14887582452213033No Hit
AAATGCGGGATTCCTAAGTTCTGCTTTCTGGAGAGGTCGTGAGTGGGCAGAG1350.14670245482107733No Hit
CTCTCAGGGACATACTCATAAGACAGAATTAAATCAGACAATACAAGAACTG1330.14452908512002435No Hit
TTACTAGGGATCTGTGATTGATAAATATTTGTTTCATGAAGGAACCTGTGAG1330.14452908512002435No Hit
GCTTTACGGGGTAGTGAAACATCAAGTCCAATTGCAGGCAACTCTCCCTTTT1320.14344240026949784No Hit
GTTTAGGGACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTT1320.14344240026949784No Hit
AACATGGAGGACAAAGTAGGTGTGTCACGGAAGAAAGTGCTTAGGGAAGATT1310.14235571541897135No Hit
AAGCACGGGCATAGAGTGGTCTTTTGAACTGGTATCTAAACATACCTGGTTA1300.14126903056844486No Hit
ATACCGGGGAGCTGATTAGTAGGTGAGATCATGAAAGAGTTGACTGCAGAGG1270.13800897601686535No Hit
AACTATGGGGGCTAATGAGTCATTGTTCCTTAATATCGTTTTCAATATGCCA1270.13800897601686535No Hit
CTGCTGGGCGGCCCAGAAGGTTATATTTAAAAAAGAAAGTTTCTCTTTTGAA1250.13583560631581235No Hit
TATGTAGGGAGCTATGACTATCTCCAAAATGCACCTCCTGGATTTTTTCCGA1240.13474892146528586No Hit
TGTTGCGGGATGTGAAAAATGTAATTTACTAAAACTGACACATGAAGAAATA1240.13474892146528586No Hit
TCTTTAGGGACTGGCCTCCCTTGCAGGCCACCTCTCTTTCTTTCTTGGAAGA1220.13257555176423286No Hit
TCTATAGGGACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT1210.13148886691370637No Hit
AATCTGGGACTATTTATTGAGTGCCTATTATGTGCCTGGCTCTGTGCTAGGC1210.13148886691370637No Hit
TGTAATGGGATTAGAAAGGCTCAATAGTAGATTTGAACTGGGAGAAGAAACA1210.13148886691370637No Hit
GTGTAAGGGATAGTCCCGGCATGGGGACCTCAACACACCTGAGCCCCAGACA1200.13040218206317986No Hit
CAGTATGGGGAAAGGAAAGAGGTCGGGAGCGCTCGCGAGATCTCGGACCACC1200.13040218206317986No Hit
GCGCATGGGACTGTTGGTGGGAAAGTGAAATGGTATAGCCACTGTGGACAAT1200.13040218206317986No Hit
AACTTAGGGAGAGGTGAAATACAGAAAGCCTTTTTTTCTTGATCTTTTCCCG1190.12931549721265334No Hit
GCTCCGGGGAAAAAGCATCTTCCATGAAACCAGTCCCTGGTGGCAAAAGGGC1190.12931549721265334No Hit
GGAGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1190.12931549721265334No Hit
CATACGGGGAGAGATCACAGTGGAGCACTTTGAAAGACCGTCGGTTGTGTGC1190.12931549721265334No Hit
GTAATAGGGCACAACGTGCATTGTGCTTCTTTCTTTGGGAATATTTGAATCT1170.12714212751160037No Hit
GATCAAGGGATCAATGATTAACGGAAGTCTTGCAAAAGAAAGAAGGGAAATC1170.12714212751160037No Hit
TCAACAGGGAGAGGTGGCAATCCTAATTTCAGACAAAACACACTTTGAACCA1140.12388207296002086No Hit
AAACTTGGGGCTGTCCAATAGAACTTTCTGTGATGATGAAAACGTGTAAAGT1140.12388207296002086No Hit
AGTGCTGGGTATTTGATGTGTTGCCTGGCAGGATACTGCAAAGAACATGGTT1140.12388207296002086No Hit
ATCAACGGGATGGTCACCAGCCCAAGGCTGCCTCATGCTCTTCGTGACTTAC1130.12279538810949436No Hit
CGAGTGGGCAACTGTAAGGAAACATAGGGGAGCCACATGACCAAGCAAGAGT1130.12279538810949436No Hit
CTCGTGGGATGGTACATTTCTGTAACTTCTTTTTAATTTTGCTGTGAACCTA1130.12279538810949436No Hit
GAGCAGGGGCAGAGCAAGATACTTAATCACATCTGCATAGACTCCTTTTCCT1120.12170870325896788No Hit
TCATTAGGGTCTGCAAGAGGGCGGCCCAGAAGGTTATATTTAAAAAGAAAGT1120.12170870325896788No Hit
CTCTGAGGGATTCCCGGGCTCGATGCCGGAAAAACACCTTGGCCCGCCGGAA1110.12062201840844138No Hit
TGTGGCGGCATTAAGTATAAAATAATTAAACATTCAAATCAAAAGGGAGAAA1110.12062201840844138No Hit
CCTTTAGGGTGGTGAGGTCAACGTTGTCACATACCTTCACATAAGGGATAGT1100.11953533355791487No Hit
CTCAAAGGGAGGATGAGTTCAACAATGACTTAATAGCATCAGAATTTTGACA1090.11844864870738836No Hit
AATGTGGGCAGCCACGACTCCTCCTTCCTCTGCGCTTCCAGTGATAAGGGTA1080.11736196385686186No Hit
TATCAGGGACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTT1080.11736196385686186No Hit
GTCAGTGGGCAAGAAATTCTTTGAAACTAATGTGAACAAAGATACAACATAC1080.11736196385686186No Hit
GCAAAAGGGCTCCCAGGTGTTGTCCAAGACCATCAAGGTAGTACGTCTACGA1070.11627527900633537No Hit
CGCAATGGGGATCCAAAGAGCCCTTCGAGCACCACGCAAGAAGATCCATCGC1060.11518859415580887No Hit
AACGCAGGGATCCCACCTACAAGTATGACACCTGTGTAACACCAGCTAGGTA1060.11518859415580887No Hit
AACTAGGGATATAAGACATTATTGAAGCCTATCACAAAATCTAAGAAGTTTG1060.11518859415580887No Hit
GGTGCCGGGGGATACTTCTAATAAATGGAATTTCATTTTATAGACTGTATAT1050.11410190930528237No Hit
GGTCACGGGACCATATGTTTCTTTTTTGTGTAGATATTAATAACATTACTCT1050.11410190930528237No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGTTTGTC1040.11301522445475588No Hit
TTTGTGCGGGACTTCTTCTCGGTCTTCTTCTCCTAAAAGGAAAAAGAGACAC1040.11301522445475588No Hit
CGTCCCGGGATTGCTGATAGGATTATATTTAATGTGAATAATTTTTAAAATT1030.11192853960422938No Hit
TGAATAGGGAAATTATCCAGTCTTAGGTAGTTCTTTATAGCAGTGTGAGAAT1020.11084185475370288No Hit
AGAAATGGGCTACCACACCTGACTTGTTCCTTATTTTAGATGTTGAAAATAA1020.11084185475370288No Hit
TTATGAGGGCGAGCATGTTTTGAAGAGGTAGTTAGTCAATCTTTATGTCCCT1020.11084185475370288No Hit
TATACTGGGGCTTTTTTCCGGCGTTCAAGATGTCGAAGCGAGGACGTGGTGG1020.11084185475370288No Hit
GAAGCGAGGATATTTAAGACTTATTGTAGGAAGTAATTTTAAATCTATTTCA1010.1097551699031764No Hit
ATAGAAGGGAAGGAAGGAGTAGGTCGGGAAGTTCTAGTAGTGGTAGTTCTAG1000.10866848505264987No Hit
GGTGTGGGACCAGGAGCCTCCTGATCTGCCAGTCCCTGAGCTCCCAGATTTC1000.10866848505264987No Hit
CTAACCGGGCGGAGACTGGTACTTGTTGGCACAGTGAACTTCTGTTTGGTTA990.10758180020212338No Hit
ACGTGCGGGTTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGA990.10758180020212338No Hit
ATCTGAGGGATTGGCTGTCATGAATATTACTGGTCTTCCGGACTTCCAAAGG990.10758180020212338No Hit
GACTTAGGGACTTAAATGGAGAGATGGCATTGGCCACTGTATTAGTCTGTGT980.10649511535159688No Hit
GGCGTTGGGACTGTTCTGCATCTTTCTTTTTCACTTAACCTTGGACGTCTTT970.10540843050107038No Hit
ACATTTGGGAATGCAGTTATTGTTTTAAATTAGAGAATAAAATGTAAAACCA970.10540843050107038No Hit
GTTCCTGGCACACTGGGCAGAATGTATCATGAGGCCACACCTAGCTGCAAAG960.10432174565054389No Hit
TTGAAAGGGAAGGACAAAGGCGGCAAGGCCAAGAAGACGGCGGCCGCCGGGG960.10432174565054389No Hit
TGCCGGGGCAGGTTTATACAGAGATGTCTAGACTGCTTCCTCCTGATTAGAA960.10432174565054389No Hit
CTTAGCGGGATTCTTCTCAAGCACACATGAAACATCCTCCAGGATAGGCATA960.10432174565054389No Hit
CACATAGGGATTTCAGGGATGGAACACGGACCAGCTGACCTTAGCGTGGTCG960.10432174565054389No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTTTGTCACTGTCTCTTATACAC950.10323506080001739No Hit
GATAGAGGGAAAGCTTTTATATGATCAAAGATAGTAAAAGATACAATATTTT950.10323506080001739No Hit
TGTCGCGGGTTGATCAGATCATGAAGTCTGACCCTGACCACTTAGCAGAGTT950.10323506080001739No Hit
AAGCCAGGGAGTGGGAACAGGCCCCAGCTCAGCCTCCGGCAGGGAGGTCACC950.10323506080001739No Hit
GTATTAGGGAATACCATTCAGGACATAGGCATGGGCAAGGACTTCATGTCTA950.10323506080001739No Hit
TCTAACGGGATGAACTCTAGGGCAATTACTATTTCTAAAAATGTATGGTGGA940.10214837594949089No Hit
GGGCTAGGGGGACTGCAATAAATGCGGTGGAGGCATCAGAACTATTTTGGCA940.10214837594949089No Hit
TCTGGAGGGAGAAGCAACAGTGTCAATATAAATTTCAAACAAGATGTAGGAT930.1010616910989644No Hit
TGGCTTGGGATTGATCATCTTGACCAATATGCTTGAGAAAGGACAGTTAGAT930.1010616910989644No Hit
TCTGCTGGGAGTGTGCTCACTCCTGTGTGATACAGCAGAAGCTGGTCATAGT930.1010616910989644No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)