FastQCFastQC Report
Sat 31 Dec 2022
SRR5848573.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848573.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233042
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCTCGTATGCCGTCT16740.7183254520644347Illumina PCR Primer Index 12 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCTCGTATGC16530.7093142008736623No Hit
CGAACAGGGAGCCAAGAGGAGGAGGAAGTCGATGATTGCTAAGCGTCGCCGA3870.16606448622994996No Hit
GCCAGAAACTGTTCATTTTCTAGTAGGGGTAAGTAAGGTAAGCCATGATTTT3630.1557659134404957No Hit
TAGCTGGGAGGCATAAATGCAAACTAATCACATTAGGGTAAATGGGTATCTA3060.13130680306554185No Hit
AAGTTGGGGGCAGCATTAATATTTAGTCAATATTATTCATTCTTAACCATAT2860.12272465907432996No Hit
AGTCGAAGGGGCTGATTTGCTGCCCTGTCTTCTCTTACTTTACTTTATCAAT2700.11585894388136043No Hit
GCGTATGGGTCCGATCTTCCAGGGGGATTAAGTGTAATTTGCCACATATAGC2650.11371340788355747No Hit
CCAGCTGGACACGTTGAAAGCACGCTTGACCCAGAAATACCAGGAACTCCAG2610.1119969790853151No Hit
CCAACCGGGGGATTTTGACCACCTTTGAGACTTTCTGATAGTATTTAAATTT2610.1119969790853151No Hit
TCTGTGGGGTTGCAGCAGTGGGAGCAATTCATCTGGAGGAGAGTGAATCGCT2560.10985144308751213No Hit
AATAGCGGGATGAATAGACAATTCTCAAGAAAGGATAAACAACCAACAAACA2550.10942233588795154No Hit
TCGTAAGGGGAGTCCCACAGGTCTGCCATTCCCATTAGGGTCTTTGACCTTG2540.10899322868839093No Hit
TTCTATGGGATACCAGGCACGGATTGTGAGCCATGCCAATACGGGCGTTTTG2510.10770590708970916No Hit
TTTTGCGGGGACATTTTAAAACGTGGCTCTGAAATGGACAACAACTGCTCAC2500.10727679989014854No Hit
ATTGAGGGTCCACTTGCTTAATAATATATCTTAACCTTTCTGCAATTTGAAG2460.10556037109190616No Hit
ACATTTGGGTTGGAAACAAAGGGGGAAAGCTGGTTTATTCCATGTAGGTTGC2400.1029857278945426No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)