FastQCFastQC Report
Sat 31 Dec 2022
SRR5848532.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848532.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185990
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGC12910.6941233399645143No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGCCGTCT12140.6527232646916501TruSeq Adapter, Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTATACAC4040.2172159793537287No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTA3760.20216140652723263No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAAC2840.1526963815258885No Hit
ATTGATGGGGTGCAGGAGGAGGGAGAGAAAAGAAGAGGTTTGAGAATAGGAA2160.11613527609011236No Hit
GATAATGGGAATTATGTATTTATTTGGGGCTAACACACAGACATGTATGTGA1960.10538200978547235No Hit
AGCACAGGGATGACAGAAATCAAATCAATGGTTAATAACCTTCTAATAAATA1890.10161836657884833No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)