FastQCFastQC Report
Sat 31 Dec 2022
SRR5848519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146616
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGC13010.8873519943253123No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGCCGTCT12400.8457467125006821TruSeq Adapter, Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTATACAC3210.21893926992961207No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTA3090.21075462432476674No Hit
GCAACAGGGAGAGTGAATCCAGGGGCTCTATTTTGACATGAATTAACTCGCA2130.14527745948600426No Hit
TTCACGGGAAAAGAATTAAAGAAAAATGAAATGTAAATATTTAAAATAAACA2030.13845692148196648No Hit
CAGTCAGGGAAGTATTCTTCACGGCAGCCAGAAGTTAATGGTGGTAGCAGCT1900.12959022207671742No Hit
GCAAATTCTCCCAAGCCAGCTTGTGAAGATGTGAAGGCCAAACCAGTATCCG1860.12686200687510232No Hit
GCAAATGGGAGAAAGAATGAATGGACACATGAACGACTGAATTAGAAATGGA1730.11799530746985322No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAAC1710.11663119986904567No Hit
GGCGCTGGGGTGCCTTTTCAAACTAATGACTTCATTATCCTGGCAGTATGGG1690.11526709226823811No Hit
TCATTGGGGAACTGGCATAGGTGAGGTCAGAGCATATAGAAAGAGCAGGTTG1680.11458503846783433No Hit
TAGCAAGGGTAGAAAAGTAGTATTGAAATTTCTAACCCATACCCCTGTGAAA1680.11458503846783433No Hit
GCTTGCGGGACATCAGTTGTTTCTAATCAAAGCTCATCTAGTTTTTAAGTTT1670.11390298466743057No Hit
AATGCAGGGTGGAGGTTCTATTTCATTTAAAAATGTATTCATTTCCCTGGCC1670.11390298466743057No Hit
GATCGCGGGGTCAGAGTTGCTATCAGAAAAAGATCCCTAGAGTTTTTTTGGA1650.11253887706662302No Hit
GAACACGGGGGGTCTGGAAAGTCTGGAAACCACCTGTAGGCCCTGGCTTTGT1650.11253887706662302No Hit
ATTTTGGGGATCTTCGTCCGGGGAGACCCATTAAGTACTATTTCAATTACTT1650.11253887706662302No Hit
ATTAGCGGGAACGTACCTGGATCCTTTGGTGGCATTCGATTATACCTGCAGG1630.11117476946581548No Hit
CTCACAGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCG1610.1098106618650079No Hit
AAGCACGGGATCCTGGAACCTTTAATTAAGAATAGCTACACTCACAAAAATA1610.1098106618650079No Hit
GCCGGGACATGCAAGACAGAAAGATACAGTATGTGGTTTATAAATGCATATT1600.10912860806460414No Hit
TGATCCGGGTTTAAAGAAATTTAACAAATCAGTGTTTTTCAGGTTTTAAAAA1570.10708244666339281No Hit
CGTGCAGGGGGGAGCGCGAGCTCGAGGAAGAGACAGGCAGCGCGCGTGAGCG1570.10708244666339281No Hit
TTCACAGGGGAATTTGACAAATTTGCCAGTGACGGGGAGTCTTGTCCAGAAA1560.10640039286298904No Hit
GTGCCTGGGGACGAACCGAAAAATAATTAATGCAGAGGAGATTTATGTCTTA1560.10640039286298904No Hit
ATTTGTGGGACATCCAAACAATATTACACTCAGATAGATAGATAGATAGATA1550.10571833906258526No Hit
TGTTATGGGCAAACAAGGGCATTACATAATAGTAAAGGGATCAATGTAGCAA1550.10571833906258526No Hit
TATACAGGGCATCACAATTAAAAGAACTAGAGAAGCAAGAGCAAACACATTC1550.10571833906258526No Hit
TGAACTGGGGAACGAGAGGGGGAGAGATGTAAGAAAAGAAAGGAAAAGTGAA1540.10503628526218148No Hit
TTCGTGGGAAGATGGCGGGTGAAAAAGTTGAGAAGCCAGATACTAAAGAGAA1520.10367217766137392No Hit
CAAACGGGGAGCAAAGCATATTCTTGAACTTTTAAGAGGAAAAAATACCAAC1520.10367217766137392No Hit
CGCGTTGGGTGAGAGCATTCAGTGCTGTGTGCTTGGTACGAAGTAGTAACAT1520.10367217766137392No Hit
AGATATGGGAGCCATTGAATTGAATAAGGCGACAGTCACTATGAAAAGGGTT1510.10299012386097016No Hit
TCGACGAGGAATGAAATATTTTAAGGACAGCATTGAAACATTGGAAAATTTG1510.10299012386097016No Hit
GTATTTGGGTATAGGTTATCTGGTAAGATTTGTCAGTACTGGAGCTGAGCCA1510.10299012386097016No Hit
GCCTATGGGAGCCAGTGAAGAAGTAGATTCCTAAATTTTATTGGGGACCATG1500.10230807006056637No Hit
ACTTGAGGGGGGCCCATGGTGTCCCCATGCCAAAGTTGCCCAAGAGAAGAAT1490.10162601626016261No Hit
TGCGCAGGGGCGGGTGATTCATGTAGCTGGAAAAGAAACCACAAACATCTTC1480.10094396245975883No Hit
AGACATGGGAGGCGGGGTCCTAGGAGCCCCTGCTTCTCCAGGCCTCTTGCCT1480.10094396245975883No Hit
TTGCGAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1480.10094396245975883No Hit
GTAAGGGCAGTTAACAACAACAACAACAACAAAATCCCATCAATAATGTCAC1470.10026190865935504No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)