FastQCFastQC Report
Sat 31 Dec 2022
SRR5847948.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847948.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163766
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGC3640.22226835851153476No Hit
CTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGCCGTCT3410.20822392926492678TruSeq Adapter, Index 3 (95% over 22bp)
GTACACGGGCTATCTATTAAGAACACTATGGAATTTCTATTCAAATTGGGTA2420.14777182076865772No Hit
ACATCAGGGGAATCCGGAGTGCCCAACATGGCGGCGGCCGTAAGGTGCATGG2290.13983366510753148No Hit
ATTAGAGGGCAGCTGGAAAGACTAGGAGGAAAAAAATTGGGTCTTGATTGAT2080.12701049057801986No Hit
CATATGGGGGAAATTACAATTCGGTAGGGTGCCAAATTGCTCCAAAAAACCA1990.12151484435108631No Hit
AAGGTAGGGAGGAATCATATTGCTTTTGTGAAACAAAGAAAATTTACCACCT1940.11846170755834544No Hit
GAGCCTGGGGTGACCATTGCTTTTCTGATGCCACGTACTCTAGAATCTGAAA1940.11846170755834544No Hit
AAGCGAGGGCTTCTATATTTTCTTAAAGCTGGTAATAGACTATAAAAACTAA1930.11785108019979729No Hit
GTGCGCGGGTATGAGTATCTGTGTGATTCTCATAGATAATTAGTCAATAGAA1930.11785108019979729No Hit
TGAAAAGGGGAATGCATTGAATAAACTGTCTTTTGAGGTGAAATTTTGGCAT1900.11601919812415276No Hit
GCAAATGGGGATTATAGCAAGTTTGCAGGACACGAGGTTAATATACAAAAGT1900.11601919812415276No Hit
TAAGCTGGGCTTTGATTTCCTATCACACCTAGCAAAAATACCTTGTACTTGG1900.11601919812415276No Hit
CGCTAGGGGCGTTTGCTTATTAGTAAACTTTATCCAGGAGAAAGTATTGGTC1890.11540857076560457No Hit
GCTTAAGGGGAGGCCACACCCTTCAAGTGGTTCATGGAGATGTGTGTCCGAG1870.11418731604850824No Hit
GCTAATGGGATTCCGAGTAAAAGTACAACATAAAGACAACCATGAAGGAATG1860.11357668868996007No Hit
GTAGGGGGGACAATCTCAAATTTCCTGCTGCTTCTCCTTAGAAATGAAAATG1850.11296606133141189No Hit
AGCAACGCGGGACTCGCTGATATGAATCCCAGAAAAAAATTTCCTGTTTTTA1820.11113417925576738No Hit
ATTCGAGGGATTAACTTGAAACTCTTATCAAAATATATTTTACCAGTTTCCA1820.11113417925576738No Hit
ATGCATTGGGGGTTTTGATTTGCATTTCTCTGATGGCCAGTGATGATGAGCA1790.10930229718012285No Hit
TCGCATGGGAAGTAGCCCCGAGCGGGAGGCTGTGGCGGAAGTGGTCGCGTTA1790.10930229718012285No Hit
TTCTTAGGGGCGCTGAGAACGCGGGTCCACGCGTGTGATCGTCCGTGCGTCT1770.10808104246302652No Hit
GGCAAAGATTTCATGACAAAGATGCCAAAAGCAACTTCAACAGAAAAAAAGT1770.10808104246302652No Hit
AGTAGCGGGATTCAGCCATGCAACAGTACCAGACCTTCTGTTAGTGCTGTGT1770.10808104246302652No Hit
CGTAGCGGGATTTCCCTCAGGAACAAGGGTTTACTAATTCATTGTCCACTGT1760.10747041510447833No Hit
ATCTGGGGGTTGGGAGGAGTGAAGGGGAGGCTGTGTCCCATCACAGGGAAGG1760.10747041510447833No Hit
TAGTAGGGGTGGCCGATGAACTCGCAAGTGTAGGTAGTGTGCTACATGAGGG1760.10747041510447833No Hit
TGCTAGGGGAATTGCTGCAGTTCAACATCAATGTTCCTTCCAGTGTCTATGC1760.10747041510447833No Hit
TTTATTGGGCTGGTTTCCTGCTTCATCTTGTGACCAAGGGACACACAAACCA1760.10747041510447833No Hit
TGTGAGGGGATCAGAAGAGATTTCAACTCAACATGAAAATTCTACTACTTGG1760.10747041510447833No Hit
GTAAGTGGGTCTTGTGTATCATGATGTTCAACTTTGCTTTTGCTGTGCCAGT1750.10685978774593018No Hit
TTCATAGGGAGAGTGTGGTCAGGCGGCTCGGACTGAGCAGGACTTTCCTTAT1740.106249160387382No Hit
TACTAGCGGGGATTTCACCAGTGATTTCAGTTAAGATCCCGTGGACTTGTTT1730.10563853302883383No Hit
CGCAATGGGATATACAGTTTATTTAGCTTACGTAAACATTTGGTGCACATAA1730.10563853302883383No Hit
TTCCTTGGGCAACCTCAGCTCACTGCAACCTCCATCTCTGGGGTTCAAGTGA1730.10563853302883383No Hit
GATTCAGGGATTGCAAGAGGCGAGTTTAAAATTCTGCAGATGGCCTTATTTG1730.10563853302883383No Hit
GATTTTGGGTGATCCCCAGTGACCACGCCCCTCTGGCCCAGGGTTCCAAGGA1720.10502790567028565No Hit
GCTACGGGAGGGCTCAATTGGCAGTCTCAGGAGCTTGGGATGCTACCAACAG1720.10502790567028565No Hit
TATAAAGGGGCCTATATAAGCCATGCGCAGGCGGAGGAGCGCCTCTTTCCCT1700.1038066509531893No Hit
ATGACTGGGGATCTTAGCTTTAAGACTGAGACTTTCATTTTAAAAATAAAAT1700.1038066509531893No Hit
ATCTCAGGGATGCCAAGATTTTGGGGTTTTTTTGTTTCCTTTTTTTGTTTGT1690.10319602359464114No Hit
TTATAGGGGGTAGGAGCAGAAACTTGAAATGTGGCAAAAACCACATGGGCAA1680.10258539623609296No Hit
GGCTCGGGCTATTCATAGACTTTGATTTTATTTATAAGCCACTTGCTGCATG1680.10258539623609296No Hit
TGAACAGGGGTGCTATTCACAATAGCAAAGACATGGAATCAACCTAGGTGCT1680.10258539623609296No Hit
GTCCATGGGAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGGGC1670.10197476887754478No Hit
TTGTTTGGGGAAGAGCAGTTGCTGATCACGATGGGAAAAGAACCTACTCTCC1670.10197476887754478No Hit
CACTACGGGATAAGGTAAAATTTTCCTGTTTTTCCCCCTTTGAAAAACTCAG1670.10197476887754478No Hit
TGTCCGGGGGGGTGAGGTGGAATTAATTTTAATTTGTTCTACATGCTGACCA1670.10197476887754478No Hit
CGATTTGGGGTCCTCTTTGTTTCTGGTTTCCTTTTAGGTTGGTATTCTTGAG1670.10197476887754478No Hit
CACGAGGGACTTAAACTTTGGTAGGAAACAAACTAAAGCAGACAAACATTTC1670.10197476887754478No Hit
TCTACAGGGAATGCAGATGCAAAATAAGTCCCTTTCCGCCTGTGTAAATTGT1660.10136414151899661No Hit
CTTTACGGGATGAATAAGCTAGTCATGGCTAGGATAATCCATTTTCATGTAT1660.10136414151899661No Hit
CGGCCGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1660.10136414151899661No Hit
AAGACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGTTTCTGCTGAGGGGGCA1650.10075351416044843No Hit
CCAGTCGGGGAGTGGTTGATGACGAACTGGCAACCAGGTTTCTTAAAAGTTT1650.10075351416044843No Hit
TATCATGGGAAGGCTACTTTCTGGGGTGGTATTGTATGTCATGTTTACCAAT1650.10075351416044843No Hit
TTCTCGGGGGCTGTAGTGGCTTCGTCTTCGGTTTTTTCTCTTCCTTCGCTAA1640.10014288680190028No Hit
CGTTTGGGGAGTGCAAAATACACAGTGAATTTTGAAGTCTTAGTATGAAAAA1640.10014288680190028No Hit
GCTGGGGAACTCAGCAGGTCTTGTTTGCCCAGACTCTGTACATTTCCAAGAA1640.10014288680190028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)