FastQCFastQC Report
Sat 31 Dec 2022
SRR5847942.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847942.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163810
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGCCGTCT6130.3742140284475917TruSeq Adapter, Index 3 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGC5950.36322568829741775No Hit
GTGCTGAGGGGCCCTTCCTCCCTCCCTCACACGCCTCCACCTCTTCCCGATC2130.13002869177705878No Hit
TGTAATGGGATAGTAATACATCTAACCAAAGAGGAGAAAGATCTCTACAAGG2090.12758683841035345No Hit
TTAGGTGGGGAGGCTTCGGAATAATCTCCCATATTGTAACTTACTACTCCGG2080.12697637506867712No Hit
ATTAAAGGGTGCCCTGCAGGGTGCTCTCCAGCAGTGGCTGGGGCCTGCGTGG2070.1263659117270008No Hit
TTCTCTGGGGGGAACGGGAGAGAGAAAGAGAAAAAGAGAGAGATCGTGAAAG2020.12331359501861912No Hit
TACGCTGGGGTGACTTAATGTTTGATTAGGAAATATTTTGTTTGGTGTAAGG2020.12331359501861912No Hit
GGCTTAGGGTGTGATAAAATCAGGCTAATTTACTTGGATTTGGGGTGATTTG2010.12270313167694279No Hit
ATTCCGGGACATCAAAGGTGCTCTTATTGCCTCCAGATGTTTCTGCTTTGGC1970.12026127831023746No Hit
ATTGGTGGGTGTTGTCTTGTGTACCTGTTGGGCTGCTAATTCATTCTGCTCT1950.1190403516268848No Hit
TCATTAGGGGGATCTCGGATAGGGATGGCACATGCAGCGCAAGTAGGTCTAC1950.1190403516268848No Hit
GACAGGGGAGGGAGTCGAGGGCAGAGGCAGCAGGCGGAGAGAGGGAGATAGG1920.1172089616018558No Hit
CAAACAGGGGAGTTCATCCATCAATTTCCTGACTCGAGTTAGTGGCATTGGT1920.1172089616018558No Hit
TTTCTGGGGACTTGCCGCAGTACTCTTAAAACTAGGCGGCTATGGTATAATA1920.1172089616018558No Hit
ACCATAGGGCATCGGCATGGACGTAGCGGCCTCCGAGTTCTTCAGGTCTGGG1910.11659849826017947No Hit
GCTAATGGGAGTTTTTCTTAAGCTTTTGGACTGGTAAGTGGCATTTATGAAA1900.11598803491850314No Hit
TCTTTAGGGGAGAGGGAGGAAAATCCAAGGTGTCATGAGCTATAGCAACACA1900.11598803491850314No Hit
AGAAGTGGGCGATCTTATATCGGGGATCTTCTTAAGGGAGATATAAATAGAA1880.11476710823515048No Hit
GAACGGGAGCAGGTTTGAAGGGAGGATCTTCTTCAGTTGTAGTTTGGAAGGT1880.11476710823515048No Hit
TACATAGGGTGATCAATCTAACCTAATTTTGACTTGTTAATAAAGAAAGGTA1870.11415664489347414No Hit
ACGTGAGGGAGTGAAGGGAGAAATATTCAGTGAAATAAATAGCACAAATAAA1860.11354618155179781No Hit
TGTAACGGGTTATTGGATTTGCACCTAACATAGATCATAATTTTTGTATCTT1860.11354618155179781No Hit
CAAGGTGGGCACTTTATGCACCAAAGTGAAAGAATTCAGTGTATCCGTTATT1850.11293571821012148No Hit
GTAATGGGAATTTGTTCATGGTTATTCTACATTTCTAGAAAGTTCTTATCAG1830.11171479152676882No Hit
GGCATGGATGCATAAAAGTGTTTCAAAAACTTCCTAGCACAAAGATAGTAAT1820.11110432818509249No Hit
GACCCTCCTGAAGACTGTCTCATCGCAAGCCACTGGTGGCTGAACACAGCAC1810.11049386484341615No Hit
GCTTGTGGGATTAAAGTAGTATTCTCAATTACCAAATGTTTCATTTGAAACA1800.10988340150173982No Hit
CTTATCGCGGGGTGAGGGAATGGAATGCACCCCAAGTGGCTAACCAGCAATC1800.10988340150173982No Hit
TTAAAAGGGACTCGTGTCTCGCTACCAGCTCCCCGCTGCCCTGCGCTCGGCG1790.10927293816006349No Hit
GTGGGTGGGGAACCGAGTCCTGGTCAACCTGTTCCTGCTCATCTCCTCCATC1790.10927293816006349No Hit
TGGCTCACTGCAGCCTCCGCCTCCTGGGTTCAGGTGATTCTCCTGCCTCAGC1780.10866247481838716No Hit
CCAAGAGGGAGGTTGCTCTAAGCAAAGTCCACGTTTTTTTAATTGAATTCTG1780.10866247481838716No Hit
ATCGCTGGGATATGTGGTTTTACACATGATGATGGATACATGATCTGTTGTG1770.10805201147671083No Hit
ATAGGGGAGCCCACCTCAGATTCTTAAATGATAGTTTGATGGGACAATCATG1770.10805201147671083No Hit
ATAACTGGGAAACAAGGCATGCCACACCATTGCTGTTCCCCATCTGGTACTA1770.10805201147671083No Hit
GAGACGGGGCAGACCCAAATATTATATTCATTTTAAAAATATTTTTCTTTTG1760.1074415481350345No Hit
CAACCTGGGAAACTTCACTGAGAGCACAGATGGAGGAAAGGCCATTTTAAAA1750.10683108479335816No Hit
TGCTCCGGGTCCTTAACTACATATGCTAAGCCCTTTCGTGCTTCCAGCTTAA1750.10683108479335816No Hit
ACTTACGGGACCACCAGCCCACCAGATAAAGGCCCCCCAAAGAGTAAACTAA1750.10683108479335816No Hit
TAGTTAGGGCTTACAGATGTCAGGTAATTTGTTTCAAAAATTTGTGAGTTCA1750.10683108479335816No Hit
TTTGTGGGGTTTTTAAAATTGTGCTTATGTTTTAAATAATAATACTTGAGTT1740.10622062145168183No Hit
CTGTTTGGGATTGCTTGAGCACAGGAGGTTGAGACTGCAGTGAGTCAAGATC1740.10622062145168183No Hit
TAACACAGGGAGGAACTGTAAGCTTTTATCTTTTAACCAACTGAACAATACA1740.10622062145168183No Hit
AGGCGGGAGGTCAAAAAACAAAGGGCAAAGCAGACATTTTAGTATACAGAGT1730.10561015811000549No Hit
TGCTGTGGGCGATCTCTACGCGGGGGAAAAAGGGGGTAGGAAAAAAGGGGTT1730.10561015811000549No Hit
GTATCTGGGAACTTCATTGTCACTTCAAAACCCCCTCAGGTTAAATAGCTTC1730.10561015811000549No Hit
CTGCCTGCCAGCAGTGCCTTGCATTTGTGATCCTACAAAGAGCCTGTGGACT1730.10561015811000549No Hit
TATTGGGGAATACCTGAAAACTGTAGCTTCATATCTTTTATGTGGTTTTCTA1720.10499969476832915No Hit
ATTTTAGGGGAGTGCAAATATCCAAGAAGAGGAAGTTTGTCGCTGATGGCAT1720.10499969476832915No Hit
CGAACGACGGGAGGCCAGTTCACTGTTCCTCACAGCTTGGCTCTTGTGCCTC1720.10499969476832915No Hit
TAGGCAGGGGTCTTGCTCTGCGGCGCGGACCCGGGGTTTGTACAAACCCTCG1710.10438923142665282No Hit
GCTTGCGGGAGCTTGACCAGTATAAAATTACAGTGGATTGCTCATCTCAGTC1710.10438923142665282No Hit
ATTGCAGGGGTACATAAAATATTTAAATCAACTTAATCTATGTACATGTTAT1700.10377876808497649No Hit
GCTTAGGGGAAAGTGAGCATCCTTGTCCTGTTCCTGATCTTAGAGGAGAGAC1690.10316830474330016No Hit
CTCGACTGGGTATCTCTGTAATGAGAAGTAATTAGCCCCAAACTGACAACTG1690.10316830474330016No Hit
TAGTCGGGGCACTGTAATTGTGATTTGCAATCCACTTATAACTCTAGTGTGA1680.10255784140162383No Hit
TAACACGGGCTATGTGCATGGTTAGGGAGCCAGGTCTGGAATATTCAGTTTC1680.10255784140162383No Hit
CATACTGGGACTAGGTGTTACAGCAATATTTTAAGAGCTTGCTCTTTTATTT1680.10255784140162383No Hit
TTATTAGGGACTCCTGTGCTCAAGCGATCCTCCTGCCTCGGCTTCTCAAAGT1680.10255784140162383No Hit
TAGTTTGGGGCTCGTGGGCTGGTGACTGGGTCTTGGGATCCCAGGCCACGCG1680.10255784140162383No Hit
AAAAGCGGGCTCTCATTCACTGTCCAACAGAAATAAAATGCAAGTCACGTGA1670.1019473780599475No Hit
GTGCTCGGGTGTGACGAGATCATTTAGCAGAAACCACTACTTCAAAATAGGA1670.1019473780599475No Hit
AAGTGTGGGAGAAATGTCTAATTTAATTTAAAGATCAATAGACAAATGAAAC1670.1019473780599475No Hit
GCTAAGGGCATATATTGCCCTGAAACCATCTGGTTTAACTTGTCCCTCAATC1670.1019473780599475No Hit
TTTCTAGGGGGACTCTAAAAGAAATATGAATGATCATTTCTCACCCCAATTA1670.1019473780599475No Hit
AACCAGGGGGGACTTTTGACAAACAGATCGTCTTCGTTCTGAATCCTCAGTG1670.1019473780599475No Hit
TTGTCGGGGAGACAGAAAGTTAGCAAGGATATCCAGGAATTGAACTCAGCTC1670.1019473780599475No Hit
CTTTGTGGGAAGATTTTGCCAAGTATGTGTTGTTGCTTGACTTCTTAAATAT1660.10133691471827116No Hit
AGCTTCGGGCGCTGGCTCCTGTTTCCAATTGATTGAGGGATGCCCCCAAGGA1660.10133691471827116No Hit
GGGCATGGGCTGACCTGGTGCATGAATTCTCAAGTACTGTATTTCACTGTGT1650.10072645137659483No Hit
GGTCGCGGGAAGGATGTTCAGATTCCTTATATAAAACCTATGTAACCTATGC1650.10072645137659483No Hit
TAGCCGGGATCTTCTCATGTGATGCTAGAGATGGGACTGGAGAAGTCACCTC1650.10072645137659483No Hit
AAGGCAGGGCTTGTATCTTGTCTCTTTAGAGAAGGAAATTTTTGTGTGCCAG1650.10072645137659483No Hit
TCAACAGGGCTCTTCGTATCCTAGAAACTCTTCAACTTGCTGCCTCTGATTT1650.10072645137659483No Hit
GTTCATGGGGATCTGCTCGAGGGAGTGGTGCTGGGAGTGTCGTGGACGCCGT1650.10072645137659483No Hit
GTAGACGGGGCCGGAACTGCTTTCTTGGGAAGATGGCGGCTGTGTCGGTGTA1650.10072645137659483No Hit
TGCAGGGCGATCTGCGTATGGGGAGGACAAGATGGTAAGTTTAGCTTTTGCT1640.1001159880349185No Hit
TATTTGGGCACCTTTTTTCTGTTATATTTGAAGCGAGTTAATTAAATCATGT1640.1001159880349185No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)