FastQCFastQC Report
Sat 31 Dec 2022
SRR5847917.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847917.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139282
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGCCGTCT3430.24626297726913746TruSeq Adapter, Index 3 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGC2820.2024669375798739No Hit
CAACTAGGGTCTTTCGCAGGGATCTGCTCATAGAGTAAAATAAAACATGACT1620.11631079392886373No Hit
TCTGCGAGGGTCACAACAAACCTGTGAAACTAGTGCTTGCATTTCTGCTCTA1580.11343892247383007No Hit
TGAATAGGGGGCTGAACACAGACAATTTAAAAAAACATGTGGTCAAAGGACC1490.1069772117000043No Hit
GTATGAGGGAGTCAACTGCCTTGAGAATATGCTATTTTCTAACCACAAGACA1480.1062592438362459No Hit
CCATCAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1470.10554127597248747No Hit
GAGGCCGAGGCAAGAGAATCGCTTGAACCCGGGAGGCGGAGGATGCAGAGAG1450.10410534024497063No Hit
GCCAGAGGGCAAGCAGTGTGGCAGATCCTTCAACTGTTCGAGCTCCTTTCGA1440.10338737238121222No Hit
CCACTGGAATATTCTAGAATAAGAGCACTGGATAAGTACACTTGAACACATT1420.10195143665369538No Hit
ATTAAAGGGTGGAAAGGTAGTGATTCTCAAAAGTTATTCTCAGTCTTCCTTC1420.10195143665369538No Hit
TGATCAGGGATTTATTGTACCTCTGGGCCTACTCTTCTAAAAATTGTAGCTT1410.10123346878993696No Hit
CTTGGAGGGATGTGGACCCCAGCAGCAGCTCCAGCTATGACAACGGTTTCCC1410.10123346878993696No Hit
GCAAACGGGAATCAGATAATTCTATTCCACTACTCAGTGACTGAGGAGTCCT1400.10051550092617856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)