FastQCFastQC Report
Sat 31 Dec 2022
SRR5847899.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847899.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108997
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGCCGTCT5540.5082708698404543TruSeq Adapter, Index 3 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGC4480.41102048680238906No Hit
TCATTAGGGCGTGGAGCTACTGCCCATGATGCTTTCCTAGTGCCTGGTTACT1940.17798655008853453No Hit
GGCGACGGGAGGAAATGTCCAATCAGGGAAGTAAGTACGTCAATAAGGAAAT1700.15596759543840658No Hit
ATTGAGGGGCATGAAGAACTCATATAATTCAATAATAGAAAAATAATCTGAT1550.1422057487820766No Hit
CGCGTAGGGGGACTCGCTTTCCGTGCGGTGCGGCGAGTGAGGCCCCGGTCTT1450.13303118434452324No Hit
GCGTTTGGGAATGGGAGACTTTAACACTCCACTGTCAATATTAGACAGATCA1430.13119627145701257No Hit
TGGTATGGGATATGCTGAAATTACAAAATGCTGATTACAAAAATGAAAAAAG1410.1293613585695019No Hit
TGCAAGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1410.1293613585695019No Hit
TAATGCGGGTCATCAGGTTGATGTGAGAAATGAGGGAGATGTCTTGAATGGT1390.12752644568199126No Hit
TCAAAGCGGGAAGGCTAATGTAATGACTGTAGATTATCTATGTCCACATTGT1370.1256915327944806No Hit
AGTCAAGGGAGGGTTCAAGGCCAAGCTGAAGTTGTTGTCCTCTATTTTCCAC1360.12477407635072524No Hit
TCTGCAGGGATCACAGGCAAGGAAGATGCTGCCAATAACTATGCCCGAGGGC1340.1229391634632146No Hit
ACGTAGGGGATTTCACATTTTAATAGAATTCTGAAATATAGATTTGACCCCA1340.1229391634632146No Hit
GCGTAAGGGGCTCTCCTTTGGGTTTCTTTCCTTTTACTTTTTCTTTTGAAAC1320.12110425057570391No Hit
GTGTAAGGGCTTCCGATCTTCACGAGGGGAGGCATTGAGGCAGCCAGCGCAG1310.12018679413194858No Hit
TTTGCAGGGGACCTTCCTGTTTATAATTTTCTAATGGAATTCTCAAATTCAT1280.11743442480068259No Hit
TAATAAGGGGATCTTCAGATGGGGCTGCCATCTCGTTCAGGCCCCACGTCGC1280.11743442480068259No Hit
GGAGGTGGCAACAAAAGTAACAGATCCCAAAACAAAGGCCAGAAGCGGAGTT1280.11743442480068259No Hit
GCACCAGGGATGAAAATTATGGGCATTTGTCCCAATGATTTTGTTTCAGCTG1280.11743442480068259No Hit
GCACCTGGGAAGTCTTGTTTCCTTAGCATGATGTATATACCCTGAGGCCTAC1270.11651696835692725No Hit
GGCTCGGGTATGTGGAAAATTGCAATAAAAGCAGAACTAAAGGTTAAAATGT1270.11651696835692725No Hit
CAGAAGGGGCGGCCCTGTGAGCCGTGCTGGCCCCACCCGAGGCCGCGGGGGC1260.11559951191317192No Hit
ACTTCTGGGAAGTAAAGGGCCGGACCTCTTGCTTCTTAAGTTATTTTTTAAA1260.11559951191317192No Hit
TCGCTGGGGGAGCCAGACAGTGACCCGGAAGTAGAAGTGGCCCTTGCAGGCA1260.11559951191317192No Hit
CAATGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGAC1260.11559951191317192No Hit
ACTCGCGGGAATCCGAATTATCAAGGTAGTCATTATTTGAAGCTGAAGTTTG1260.11559951191317192No Hit
TAAACTGGGGTAACATTTGTAGATTGGATAGCATTTTTATGATTTGATGAGT1260.11559951191317192No Hit
ATTCACGGGCTGCAGAGGTGGCAAGAAGATGGTGGTGTTATGATCATAGGCT1250.11468205546941658No Hit
TATTGAGGGACTTTCATGGGATATTAATATTGATCTTCATTTGTAGTAAACT1250.11468205546941658No Hit
GCACATGGGCTGTGAAGTTCAGTACCACATTATTTTTCATTTCGACCACAGC1240.11376459902566127No Hit
GCACCCAACCCCACATCTGGGTGGCCAACTTGGCAGGACTTGGCCAGCAGCT1230.11284714258190592No Hit
GAAACAGGGCGGGTGTTGGAATCCACACCAAACAATGGCTACCTCTATCACC1230.11284714258190592No Hit
GCCCAGAGCCTTGAGTTTTGATTCCAGTACCTAGTCATTTTCTTCTTCACTA1230.11284714258190592No Hit
TTCCCGGGATGGCTTTGAATGCAGCCCAACACAAATTTGTACACTTTCCTAA1230.11284714258190592No Hit
GCCATTGGGGGAAATATGATGTTGGCAGAAGTCTAGTGGCCTGACATATGAG1220.11192968613815059No Hit
GCTTTGGGGGAGACGCTGATGGGAGGATGGACGTACTGGTGTCTGAGTGCTC1220.11192968613815059No Hit
GTTGGAGGGCTTTCACCACCCACACCCGGCTGCGCTTCTCAGTGCCATATGT1220.11192968613815059No Hit
AAGCCAGGGGAATGTAAAGCCAATTTGATGAAATGGTTCAGGTTGTAGCAGT1200.11009477325063992No Hit
TGAGGCGGGCAACCGCTACAGCTCCTATCAGAAAGGGCAGCGCCCGTGGAAT1190.10917731680688458No Hit
TTAGGCGGGTAGCCTAAGTATACTAAAAATAGAAGGTTATTTCCTTCACTCA1190.10917731680688458No Hit
TTCAAGGGGGAATAATGAATATAAGAAAAAATGACGAAAAGTGCTAGGCCAG1190.10917731680688458No Hit
AATGCTGGGATTACAGGTGTGAGCCACTGCACCTGGCCTGTAGTAGCTTAAA1190.10917731680688458No Hit
TACGCTGGGGCTGGTGTTCGGCCTGGGCGCGATCCAGGTGGTCGGAGGCTCG1190.10917731680688458No Hit
TATGCTGGGGTTGAAGAGCGCATCTGGTGGCAGCCATGGATCCGCGGCCAGG1180.10825986036312926No Hit
ACCTTGGGAGTCAACATGATGGACGAGTTTGCCAGATATGCCAGGCTGGAAA1180.10825986036312926No Hit
CCGTCAGGGGCCATTTTACTTTTTGCTTCTATGAGTTTGACTGCTCTCGCCA1180.10825986036312926No Hit
TATGTGGGGGCACCGTCATCACACACACTTCTATTTATATTTCTTTCTTTGA1180.10825986036312926No Hit
AAAGAAGGGATGTCGTCACAAGGATATCTGGCAAAGGAAACCAAGCTGCTTC1180.10825986036312926No Hit
TTTTTGGGGAACAAAAGGCGACCTGCTGAGAAGTCCTTGTGCTCTGTGCTTT1170.10734240391937393No Hit
AACATTGGGGTTGCTACTGAAATGATGTACTTTGACGTAATAAAAAATCAAA1160.10642494747561859No Hit
GGCTATGGGCTCACATAAACTTAAGGCAAAGGGTTGGAAAAAGACACTTCGT1160.10642494747561859No Hit
TTGTATGGGATTTTCTGTTCGTGCATGAATTCACTTGGGATAATGGCCTCCA1160.10642494747561859No Hit
ATCGCGGGCTGTAAAGGAGTCTTGTTTATCCTCTAATGGCCAGGCTTTTGGG1160.10642494747561859No Hit
GTGTGAGGGCATGAAGCATGGAGACCTGAATAAGTTCCTCAGGGCCCATGGG1150.10550749103186326No Hit
CGTAATGGGTTAGCATGGGCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.10550749103186326No Hit
GTGTGGGGTAATTAAAGTATTTCATCTTTTAAAACTCAAATAAAAAGTCCCG1150.10550749103186326No Hit
TTACGCGGGCTGGTAACAGTGCCTCTCTTTTTTCCTAATAGGCTTTATAGAA1150.10550749103186326No Hit
CAGGTAGGGCGATCTTTTCGGGGGTGTCGGAGGGATCTCGAACTTTCAAAAT1140.10459003458810792No Hit
CAAACTGGGCACGCTGGTGAGGGAATTCAGCAAAGACCTGTGAAGCTACAGA1140.10459003458810792No Hit
TTTAGTGGGATAATAATGGGAGACTTTAACACCCCACCGTCAATATTAGACA1140.10459003458810792No Hit
CATCATGGGAATATAAATTATTTTTGAGCTCTCACAGAACTTTGCTGAGATA1140.10459003458810792No Hit
CAACCAGGGCTGCAGGCTAATGGCAGGAAATGGTCATATTTGGGGTTTTTCC1140.10459003458810792No Hit
ACAGATGGGTGGAATAATGACCCTTTAATAGAAAAAGAAAATATTTCTAACA1130.1036725781443526No Hit
GCTACCGGGAACATTCAGCAATATCTGATGACCAATAATGTACCGGAGGCAG1130.1036725781443526No Hit
GGTACTGGGAGAATTTAAAATATAGAATAAAGTATATACCAAGTACTTAAAC1130.1036725781443526No Hit
GCACCCGGCTTGAATCATCCTTTTGTCCAGTATTATATCCATGCTGTATATG1130.1036725781443526No Hit
CTATCTGGGGCATTCCTCTCTGTTCATGGAGCTTCTTTGAGGCTTGAAGTTT1130.1036725781443526No Hit
CAAAGTGGGAATACTGTTCTGTGTAGCTTAATCTATTTCTTTATACATCTCT1130.1036725781443526No Hit
CATACAGGGAGAACTGCATCAGGATATGGTGGTACCAGGAGACGATAAAGTA1120.10275512170059727No Hit
GAACAGGGAAAAGTGAGAAATTTATAACATGTATAATTCATTAAAAATAACA1120.10275512170059727No Hit
ATAGCAGGGCTGCTGTAAGACCACCAAAAATGAGTCAGAAACACAGAAGACT1120.10275512170059727No Hit
TACATTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1120.10275512170059727No Hit
TAATGTGGGCTCAATAAAGGCTGTTGCCTAAGCATGGCATACTTCATCTGTT1120.10275512170059727No Hit
TACAGAGGGGCCACATAGCCCTCGTAGTAACAGCCATTCTCATCCAAACCCC1120.10275512170059727No Hit
GTTGATGGGCACTTTACAAAGAAGCAATCACTCAATGAAACTGCTGGCTTTT1120.10275512170059727No Hit
CCAGCCTGGGCAACAGAGCAAGACCCTGTCTCAAAAAATAATAAATAAATAA1120.10275512170059727No Hit
GTTAATGGGCTCCCTCTTGGACTTGTTTTCCACCCTCTGCAATGACCTTGTC1110.10183766525684193No Hit
TGCATGGGGGAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGGG1110.10183766525684193No Hit
GGGTTGGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA1110.10183766525684193No Hit
AAGTGAGGGTCTGCCTGGTTTTGGTATCAGGATGATGCTGGCCTCATAAAAT1110.10183766525684193No Hit
ACGCACGGTTCATGGAGGGGCTTTAGCCATCAGCTTTGTACATCATCATTTT1110.10183766525684193No Hit
TCAACTGGGTCACTTCAGCCTCCAAGCTACTTTGGCTTCTGCCTGGCTCAGG1110.10183766525684193No Hit
ATTCGCGGGTTGCAGAGGGGAGTCTCAACTCATGAGTTGTTATACTTTCATT1110.10183766525684193No Hit
GGTTTGGGCATGTTAGAACATTTCTTGCTATGGTTAAAAATGCTGCTTCCTT1110.10183766525684193No Hit
CTCAAAGGGGGTCTGATTGGTTGCAGAAAACAGCCAACCAGAGGCTGAAGTG1110.10183766525684193No Hit
CGTCGCGGGTAACAAAGAGTAGAACTATTACCAGTTTTATGTGTTGCTGTTT1100.1009202088130866No Hit
GCAGTGGGGATCTGTGTTACATTGCCATTATATCAGTTTTGCTTGAAATAAG1100.1009202088130866No Hit
TCTGCAGGTACCCTGAGAAAAGTGAGAAGAGAGATTGCAGCCCAGGTCCCTG1100.1009202088130866No Hit
TATACTGGGATGTTTTTGTTTGAGGATATCTGTACAAGTTAAATCCCTCACA1090.10000275236933126No Hit
AATAGCGGGCATGAGAAGTTAAAGAATAAAACAAAATGTACCCTTAGCTATA1090.10000275236933126No Hit
GTAAAAGGGCTCCACGCTACTGAGCAACTTGTTAGAAGAGATGAAGGCGACG1090.10000275236933126No Hit
AATAGCGGGAATTCACACTGTATATAAAATGGAAACAAACTGATTGCTCAGA1090.10000275236933126No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)