FastQCFastQC Report
Sat 31 Dec 2022
SRR5847800.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847800.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43056
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCGTATGCCGTCT13803.205128205128205No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCGTATGC10842.517651430694909No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACT2440.5667038275733928No Hit
CTTATACACATCTCCGAGCCCACGAGACACACAACTATCCTGTCTCTTATAC2020.469156447417317No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTA1460.33909327387588256No Hit
GAAATTGGGGAGGAACAGAATCAATGAAACAAAACCAATGTTAGGGAATTGG1220.2833519137866964No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCCTGTCTCT1190.2763842437755481No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTAACTCGTATGC1070.24851356373095504No Hit
CTTATACACATCTCCGAGCCCACGAGACACACAACTACTGTCTCTTATACAC1020.23690078037904125No Hit
CACCATGGGCTGATAGATGATGCTGAAAACCCAACATGGAGTTTTTTAGTCT980.2276105536975102No Hit
TCATTTGGGCCATCAGTTGGGGTCATACCACTAGTCTCTGTCCTATACCCCA920.2136752136752137No Hit
GAACGGGGGGTGCGCGCCCCGGATCCGGCGGCCACCCAGAGGAGAAGGCCAC890.2067075436640654No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTC800.18580453363062058No Hit
GTATGTGGGGGTCAACTTAAGATTTGATTCAAGGCATTGATTTCCTAATTAA780.18115942028985507No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCGT760.17651430694908954No Hit
CTCGCAGGGGAGGGCTGGCTGGGCTCCAGCTGGCCTCCGCATCAATATTTCA740.17186919360832406No Hit
GTAATTGGGACCTAGCCCGGTGCGTCTGCATGCCCTGGCAGGGTTCCAGCAG730.16954663693794128No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTACTGTCTCTTA720.16722408026755853No Hit
CGTGCCGGGTAGGGTAGGGGATCTTCTTGCGGGAGATTTCAGATGTGAAATC720.16722408026755853No Hit
TAGAAAGGGGATTAAGACATTTTTGGTACTTGCATTTGACTTACGATGTATC720.16722408026755853No Hit
GTGACAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC720.16722408026755853No Hit
GAGCTGGGGAGAATAAGGTCCGAGAGACCCCACGAGTGTGCGTAGGGAACGG690.16025641025641024No Hit
AATTTTGGGAAGGAGGCGATCTGCAGCTCACTTAAGGCCTACAACCACCACA690.16025641025641024No Hit
ACAGTTGGGACATCAACCTGGAGCTGCTGGAGTGTGTCTACCTGGTGTCTGC670.15561129691564474No Hit
TAGAGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAA670.15561129691564474No Hit
GCTATAGGGAGCAGCAGCAGCAGCTCGGGCGTGGGCAGCCCGGCCGTGACGC650.15096618357487923No Hit
ATAGCGGGGAGGTATCAATGTAAGGACACAAGAAACATGAAAAGGAAACATA640.14864362690449648No Hit
GTTACTGGGATATCCAGAATATATAAAGAACTCTTAGAACCCAATAATAAAA640.14864362690449648No Hit
ATACGAGGGCATCAAAAAGTCTGAAAGAGCACAAATAAAAAAATCTAAGATT640.14864362690449648No Hit
TTCGGCGGGCACCTGGGAATGGAATGGCCAGTGAACACCATCCCAGAAGCAT630.1463210702341137No Hit
ATTAAGGGGACAGTGACTTCTTAGTAAGCCCCTACATTATATGCATACTTGT630.1463210702341137No Hit
ACATAGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC620.14399851356373095No Hit
TCTAGAGGGCACAATCATGGCAAGCCAACGCCACTTATCCAGTGAACCACTA610.1416759568933482No Hit
CGCCATGGGGAGCGCTTCGGCGAGAGCCCCTTCCGCGACGAGGAGGAGTTGC600.13935340022296544No Hit
CTGTTTAAGGGGCAGGGCAGCATGAGGATGACTCCTTGGAGCTACAGGGGCT600.13935340022296544No Hit
TTTGCGTGGGGATCACCAAAGGTCAGATTGTCATCAATGGATTATGAAAGGC600.13935340022296544No Hit
AAACTGGGATAGCACCTCCTCCGAGTAGCCTATCAGGATCCCAGCAGTGGCT600.13935340022296544No Hit
ATAACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA590.1370308435525827No Hit
TTGAAGGGGTGAAGAAAATTGAGCTCTCTGAAGTGACGGATACATTATTCAA590.1370308435525827No Hit
ACTCCTGGGAAACAAATAAACCAAAAAACAAATAGAGGGAAGGGGATATAGC580.13470828688219993No Hit
ATTTGCGGGACTCATTTTATTCATGCCTTGGTATGTAATTTGTCAAAATATC570.13238573021181718No Hit
GCATGCGGGTGAGTCTTACAGCCTGTGGGATGTTCTCCCCAAGCTTCTAACT570.13238573021181718No Hit
CATGCTGGGGAAGTTGGAGTCCCATCCCCCAAGGCACATTTTTTTTTTTTTT570.13238573021181718No Hit
CAATTAGGGGTGTGCTCATTGTGTCCCAGACCCATGCTAAGTCATCTATATG570.13238573021181718No Hit
CGTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA570.13238573021181718No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTAT570.13238573021181718No Hit
TAAAGTGGGCATTGAGGATTGGTATAGCCATGATTTCAGTCATAGCAAGCTT570.13238573021181718No Hit
ACTACAGGGGAGACCCTGTCTCTAATAAAAAGAAAATGCATAGTATTTATTT560.1300631735414344No Hit
ATTGCTGGGGTATACTCTACTAACAACAGTAATCAACTACATTGACATCATT560.1300631735414344No Hit
AATTGTGGGCTACTCACTCTCACTGCCCAAGAACTATCAAACTCCTGAGCCA560.1300631735414344No Hit
ATTTTAGGGATTGTGGAGCTGGCTGAGGACGCCAAGTGGCGGGTGCGGCTGG560.1300631735414344No Hit
TCCGACGGGGGCCCCCAGGGGAAAGGGCTGTGAGCTGCTCCGCCATTAAACT560.1300631735414344No Hit
CTAACAGGGGAGTTGCTGCTGCAGCTGAGGTACAGCGGCGGTTTCTGAGGTT560.1300631735414344No Hit
AAGTTAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG550.12774061687105165No Hit
GTTAAGGGGGCTAACTGATAAGAGTGGAGGAGGAATGCCTTCTCCTAAGAGT550.12774061687105165No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCGTAT550.12774061687105165No Hit
GTAAAAGGGACAGTATTAATGTTTGATAAAGGTATATCCCAGTTAACTACAC550.12774061687105165No Hit
TTCGTGGGGATTTGGAGCAGGGTGGTCACACTGACCTTGCAGAGTTCTGAGA550.12774061687105165No Hit
TCTTACGGGATAACCAAAACATATCCAGATGACACCTACAGGTTATCCAGAC550.12774061687105165No Hit
GTACGTGGGAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATT540.1254180602006689No Hit
ACTTATGGGTATCCTTTGAAAATAAGGATTTTAATTTCTACATGTTAATAAC540.1254180602006689No Hit
GTAGCGGGGAATTCACCTGCTCTAAACAATTGTTACTGACACACTGCTTTTA540.1254180602006689No Hit
GGTCTAGGGGGAGCTAACTATCCTAAATATATATGCACCCAATACAGGAGCA540.1254180602006689No Hit
GGCGCTGGGATGTGACACCAGAGCTCACGGGAGATTCAGAAATCATTGTCAA540.1254180602006689No Hit
CGGTCAGGGATTCATTGTGAATCCTGTAGCTTTTCTAGTTAACAAAAAATCG540.1254180602006689No Hit
CTGCTGGGATAGTGACTAGACCATTTTACATTCCCACCAACAATGTACAAGG540.1254180602006689No Hit
GGGCATGGGATCTCCACTTCTTCGCGTTCGGTGATGCTGCATTACCGCTCAG540.1254180602006689No Hit
TTAGTGGGGGGGCCAGGACCCCCTCCTGCTCCTGCAGGCCCTGCAGACCCTG540.1254180602006689No Hit
GGTAATGGGAATAATTGTATCTCTGCAAGGTAAGTATTATTATCCCAGTTTT540.1254180602006689No Hit
GTTCAGGGGCACCTAAGGTGTCTGCATCCTCCTGCCAACGCATGGGCTCCAC540.1254180602006689No Hit
GTAGTCGGGTATAGTCTTCAGCTCTATGACTTCTGTTAGATATTTTTTGTAT530.12309550353028614No Hit
GCAATGGGAGTTCCAAGTTTCAAAGAAATGTCAACATTTTATTCCATTCAAT530.12309550353028614No Hit
CTAAGGGTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC530.12309550353028614No Hit
TACTGGGGGAACGAATTAAGAAGAAATAGAAAATTTAAACAAGCCAATAACA530.12309550353028614No Hit
TTAGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA520.12077294685990338No Hit
CGGCGTGGGATGTCCAGAGTCCGTGAGGATTGGGAATCAGTCTAAAGCTGTG520.12077294685990338No Hit
GCTATGGGGATAGCCTTGAGGCTAGATTATCTTTCCTTTGTAAGATTATTTG520.12077294685990338No Hit
TGGCTAGGGAGCTAAAACCCACTCTATGAGAAGGAAGATTACTGAAAAGCAT520.12077294685990338No Hit
CTTAAAGGGACCTTTCCCTTCTCTCCCCAATACCCTTCCCAGCTTCTGGTAA510.11845039018952062No Hit
ATAGCAGGGGTAATTTTGTCCATCAAATGAATATGTGTGAAAACATTCCCTC510.11845039018952062No Hit
CGTTTCGGGACCACATAACTGGAAGTAAAACCTCCTAAGCAAATGCAAAAGA510.11845039018952062No Hit
GCTACGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC510.11845039018952062No Hit
TAACATGGGTATAATTATTTATGGTACAATTCTTGTACTTTAGCAAATCTGG510.11845039018952062No Hit
TCAAGTGGGATTCTTTTAGGCATTGATGCTAACACAGCAAATAAAACGGAAA510.11845039018952062No Hit
TACACCGGGACAACAAAAGCATTTGTTGACACTCGTGCTGTTAAGAGATTTG510.11845039018952062No Hit
CGTGATGGGGACCCTCATTAAGCTGCAAAATAGTTAACATTTCAGGGATTAT510.11845039018952062No Hit
TAGTGCGGGACCCCAGAGATTTAAGAAGAAAACCTCTAAATTTCAAAATTAT510.11845039018952062No Hit
CGAACGGGGCGATCTGACAGAGGGAAGCAACTGGATTTACAGATTTATTGTG510.11845039018952062No Hit
GTCAAAGGGCATACCCATGTTGATAGAGTTATTACCACACTCTAGGTCAATT500.11612783351913787No Hit
TAGAATGGGCCTCCACCTCTGCTCTGACCTCTCCCCATCTCTCTGCCCCTCC500.11612783351913787No Hit
TCTCCTGGGTGAGTAATGTCTGTTTCCAGGGTTCAGAAAAGGCAAACTCATG500.11612783351913787No Hit
TTGTTAGGGCCGGCTGTGCACCCGGCCCTTCGGGCTGCTCAGCGTGCGCGCA500.11612783351913787No Hit
GGTACAGGGCTACATTTTCGTTTATAAATATGTTTGTGGTATAAAAAAATGA500.11612783351913787No Hit
GTACAGGGGAGTTTAAGAGCAGAACAGATCTTTTACTTTGGCCGCTTGAAAA500.11612783351913787No Hit
GGTTGAATCTGAGATTTTGACTGGCTGGTTTATGAAGATTTCCTGCTTTAGG500.11612783351913787No Hit
AATTAGGGGTACTGCGTAAATAACAAAATTAAGACAGAAATAAATAAGTTCT500.11612783351913787No Hit
GCCGCAGGGGATCTCAAGATGGGATTGTTTTAGATACACTGTTTTTATAATA490.1138052768487551No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCGTATGCC490.1138052768487551No Hit
CTGTTCGGGGGAGGCTCTGTGACTGGGGCAACCTGCAAGGAGCTGGCCAGCC490.1138052768487551No Hit
GATCAGGGGGCTTCGGAATAATCTCCCATATTGTAACTTACTACTCCGGAAA490.1138052768487551No Hit
CTCGTAGGGGAAAGGGATGCAAAAGATGCTGTTGATTTGATGAAGGCGCTGA490.1138052768487551No Hit
GTCAAGGGGGGTGAATACTTTTCAGGAATTCTTCCAGTTTTAAAATAGCCCC480.11148272017837235No Hit
GCTGTCGGGAAGGAGAAGCTGTGCTACGTCGCCCTGGACTTCGAGCAAGAGA480.11148272017837235No Hit
CAATAGGGGAGTGAAGAATGTCAATGGTAGTTTAATGGGAATAGCATTGACT480.11148272017837235No Hit
AAGAATGGGGACTTGCCATGGTCGTCTTTGTCTGCCGGCCTGCTTCCTTGTA480.11148272017837235No Hit
ATGTCAGGGAGCTCACCCTTGTAATCCCAGCACTTTGGGAGGCTGAGGTGGG480.11148272017837235No Hit
ACCTAGGGGATGTCACAGATCCGCAAAGGCCACGACACCATGTGTGTGGTGC470.1091601635079896No Hit
GGATTGGGGGTAGGTTGGGAAACATTGGAGAATGGTTTATCCAGTTTAATTA470.1091601635079896No Hit
AGTGCTGGGACTGGGTTTCCCTTGATAAATCACCTTCCATCTTTCATACAAA470.1091601635079896No Hit
TCGCTGGGTGTAAAAAACCTAATACTTTCCAGTCTTCTCAGCTTCCTGATCA470.1091601635079896No Hit
AATCGCGGGCAATCTTTGTGATATTACTTCGCTATTAAATAAAGAAAATTGG460.10683760683760685No Hit
CTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCTGTCTCTTATA460.10683760683760685No Hit
CGGCCAGGGAAGTACTGGGGAGAATGCCTCATGATGACCGAGTGTAATCGTA460.10683760683760685No Hit
GCACTGGGGAGTGAGAGAGCTGCACTCACCTGCAGACCCAGTCAGATGGCTT460.10683760683760685No Hit
AGCCACGGGCATTCTCAAGGAAACCTTATCATAAGTGTAGTCTGTTGATGAG460.10683760683760685No Hit
AGCACTGGGATTTATAGTTTATAGAGAATTTTCTTATTTGATCATTCACTTT460.10683760683760685No Hit
AACCAGGGGGGATAGCAAAGAATAATTTAGAGATATTAAATGAGAAGATGAC460.10683760683760685No Hit
CCACCAGGGTGAGGGTTCCACCCCCATGACCCAAACACCTCCCACTAGATCC460.10683760683760685No Hit
AGGTAGGGGATCTAAAACTGGCATGGAATAAACCAGTAACTAATATTTCAGC460.10683760683760685No Hit
TGAACAGGGCCAAGCTCACTTGGGCCAAGAGGTTTGAACATGAGCTTTTACC460.10683760683760685No Hit
GCACCTGGGTCTTACAAAAATTTAATGGCACTATTTGTTCTGCCTTTCCATA460.10683760683760685No Hit
CTTTTAGGGCTTTGCAGTGAGTCTCAGTGTAACAGTGGCTCTGGGGTGCATG460.10683760683760685No Hit
GTAAATGGGATCCTCCAGGGATTGAACATTTTCCAATTATCTGGTCTTTTCC450.10451505016722408No Hit
GGCTTGGGGAATCTTATTTCTAAGCTTTGTAAAATTTGTAAATTTAAATGAT450.10451505016722408No Hit
TATTGAGGGGGACCACACACAAGTGTTATAGGAGGAGTCTGGCCCTTGAGTA450.10451505016722408No Hit
AGTATGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC450.10451505016722408No Hit
TAGTTAGGGAACCTGTTACAGCTCATTGTTGAGCTGAATTTTTCCTTTTTGT450.10451505016722408No Hit
TAACTGGGCTTTAGGTAGTGAATTAGTTAAAATGAATCTAAATTGTGTGTTT450.10451505016722408No Hit
GCCTAGTGTTACTGATATTCGAAGACCTCGGCTCTACCTCCTTCAGTGGCTA450.10451505016722408No Hit
CGTAATGGGGTATCCAAAAAAAATTATTGCCCAAAGCAGTACCATTGAGCTT440.10219249349684133No Hit
TCGTCCGGGGACCCTCCCTAATTTCCACTCCCCCCACCCCACTTCGCCTGCC440.10219249349684133No Hit
GAGATAGGGACTCTAGCGTAAAGGACCCAGCCAAGAAATTGTAAAATAGATT440.10219249349684133No Hit
TTAGGTGGGCGCACCCACCCTGTTCTCTACCTCTTCTATGAAGACATGAAGG440.10219249349684133No Hit
GAATTAGGGGGAAGGTGAAGATGGCGGTGGCCAGGGCCGGGGTCTTGGGAGT440.10219249349684133No Hit
TAAGCAGGGAGGATATACAAGGTAAAGGACCTGGATAATCGAGGCTTGTCAA440.10219249349684133No Hit
TACTCCGGGAGCCAAGCCACTCCATGGCAATGAGCTCCGAAGACAAAGTGCA440.10219249349684133No Hit
AGTTATGGGCTTGACAGAGCAAAGATTTTAATTTTATAAATTATGATTTCTT440.10219249349684133No Hit
CACATGGGGGACGGATGGACTTATTAATCAACTTCTCGAGAAATACTAAAGC440.10219249349684133No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)