FastQCFastQC Report
Sat 31 Dec 2022
SRR5847785.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847785.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42846
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCGTATGCCGTCT13543.1601549736264762No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCGTATGC13063.048125846053307No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACT2160.5041310740792606No Hit
CTTATACACATCTCCGAGCCCACGAGACACACAACTATCCTGTCTCTTATAC1740.40610558745273767No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCGT1550.36176072445502494No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCCTGTCTCT1460.34075526303505577No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTA1330.31041404098398917No Hit
CTTATACACATCTCCGAGCCCACGAGACACACAACTACTGTCTCTTATACAC1050.24506371656630727No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTAACTCGTATGC960.22405825514633806No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTAT830.19371703309527144No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTC670.15637399057088175No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCGT670.15637399057088175No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCGTATGCC610.14237034962423564No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTACTGTCTCTTA600.1400364094664613No Hit
TCAGCTGGGGGTTGCGTCCGGGGGAGGGGATGGAAGGGCCGCTGTCGCCAAG540.12603276851981515No Hit
CTGAATGGGTTCAGATTTCCTCTTTGCCTTCCAGATCCAGTTTTACTACTCA530.1236988283620408No Hit
AACGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA530.1236988283620408No Hit
GTCTAAGGGCTGGCCTGGAAAATTAGTAAGAGAAGACGACGTTCACAATTCA490.11436306773094339No Hit
GACAAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC490.11436306773094339No Hit
TGTGCAGGGACCGAAAGTAAAAATGGAGCAACGTGAGTGACCGCCCAAGGTC490.11436306773094339No Hit
ATTTATGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCT470.10969518741539468No Hit
ACATCGGGGAGGAAGCTTTTTGGAGAAAAGAAAAAGAACAGCAGCTATTACA470.10969518741539468No Hit
CTACAGGGGGAGGCAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCT460.10736124725762031No Hit
GCATTTGGGAACTCTTTAGTATCTTCTCTCTTACCTATTTGAGCCCCTCTAG460.10736124725762031No Hit
ATAAAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA460.10736124725762031No Hit
GCTGCAGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA450.10502730709984595No Hit
GTCGCGGGATGTAAAATGGCTTAGAATAACGACTGGCAAATCTTAAGCGCTT450.10502730709984595No Hit
CAGTATGGGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACT450.10502730709984595No Hit
GCAAATGGGATGTCAGCAGAGTCTCCAAGCCCCACGAACCCTGAGGAGTGGA440.1026933669420716No Hit
AACGAACGGGGTGGGCTGGGCCGGGCCCAGGAAGTGAGGCGGCGCTGGGCCA440.1026933669420716No Hit
CAAACGGGGAAGTATCACTTTTTGAAGTATAAATGTTGCCAGGTAATTTATT440.1026933669420716No Hit
GACTGCTGGGGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC440.1026933669420716No Hit
ACTCCTGGGATGGACCCTAAAGGGACCCTTAATAGCCATCTCCTCCACAGCA430.10035942678429724No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACACACAACTATCTCGTAT430.10035942678429724No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)