FastQCFastQC Report
Sat 31 Dec 2022
SRR5847759.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847759.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89220
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATGC6170.6915489800493163No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTCCACCATCTCGTATGCCGTCT5380.6030038108047523No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCTCTTATACAC3470.3889262497197938No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCACTGTCTCTTA2880.32279757901815737No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACC1420.15915713965478592No Hit
AGTGGCGGGGAAAGAAAGTATCTGTCAACAGTGACTTCTGTGTCCAGTGAAA1360.15243219009190764No Hit
ACATCAGGGAGCTGGGACCACTTGTGAAACGGCTAGTTGTGCCTAACAGACA1340.15019054023761488No Hit
ATACGCGGGTCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1320.14794889038332212No Hit
GTTGCCGGGCTCTCCACCTTAGTACTTTAGACTAATTGTGTTTGTCCGTCCA1290.14458641560188298No Hit
GTAGTGGGGAATGTATCCATGCATTTGGATACTTCTGAAAATAATGACTAGA1280.1434655906747366No Hit
GCTAAAGGGGCTTTTCCGTGCTACCTGCAGAGGGGTCCATACGGCGTTGTTC1280.1434655906747366No Hit
TAACTGGGGCACAAGTGGAAGTGGACTTTAAAGTATCACAATGTTAAAATTA1270.14234476574759022No Hit
GTAACAGGGCATTCTCTCTCCATTTACTTCTGGTGGTTACCCTGACTCTTGA1220.13674064111185832No Hit
GACGATGGGTCAGGATTTTTCTTTTGACCTAATTTCCAGTCCACTACTTCAG1200.13449899125756556No Hit
GAACTGGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC1190.1333781663304192No Hit
TTATGTGCCTGCTCCTCTTAGTAGCTCTTCTTTCTTGGTACTGCCTTGCCAG1160.13001569154898004No Hit
AATTAAGGGCAGTTCTGTGTGGGGGCAGCCAAGGGGGTGCTGAGCCCGTTTG1160.13001569154898004No Hit
GGTCCTGGGACAAACAAAGTGTTACATCTTCTGCATAAAATTACCTACTTAA1150.12889486662183366No Hit
GCAATCGGGGAAAAAGAATCAGTGCAAGAAACGCTGGCAATTCCAAAAGGCA1130.1266532167675409No Hit
TCTACTGGGGGGATCCAAATACATAAAATAGATATGTTTTACCAGTAAATTT1120.12553239184039452No Hit
ACGCTCGGGTAGATAGGAATCGGCCATATGACGAAATGAGATCAATAGGAAA1120.12553239184039452No Hit
AAGCTCGGGTATCTAAGTGTATCTAAACATAGAAAAGGAAATGCATTGTGCT1100.12329074198610178No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTCCACCA1080.121049092131809No Hit
AGACAAGGGAGCCAGGCTTTCTTAGCCATTCTTTGCAGCAGAAGATTTCTTG1070.11992826720466264No Hit
GGTGCTGGGGCATGAACCCTGTGTGAATCAGACACTCACGGACGTTCTGTGT1070.11992826720466264No Hit
TCTTTCGGGTGACTACAGACACTTGCAAGCTTTCCTTTTACTTTCCTGTGAT1060.11880744227751625No Hit
TTGCAGGGATAGCGGCCGCGGGCGGCGGCGGCAGCGGTTGGAGGTTGTAGGA1060.11880744227751625No Hit
CGGTCTGGGAAAGGAAGAAGAGTCCACCTTGCGACCGTATCCGCTAGCGCGG1060.11880744227751625No Hit
GGCCCAGCTAATTTTTCATTTATTTATTTTTTTGTAGAGATGGGGGTCTCCC1060.11880744227751625No Hit
GGTTCCGGGAATCTTTGAGATACATCCTGTCATCAGTATATTTCAGAATACA1050.11768661735036988No Hit
GCACGGAGGGAATGAGTACCTGGATGAAACTACACTAGTGGTGGTGGAGATG1050.11768661735036988No Hit
TCACACGGGTAGCCTTGGTCATGCTTCCTAGAATCTACTTGTCCAAGTTCTC1050.11768661735036988No Hit
TTAAGTGGGAATATAATGCACTCTCAAAATTGTGAAATTAAAGAACTGAAGC1040.11656579242322349No Hit
GCTTCAGGGCTCTTAAAGGTACTACTACCTATCTAACAAAGCTTGAAGCCCC1040.11656579242322349No Hit
AAAATGGGGTTCTGAACGAATGAGCACACTTTAACCAGTTATTTACAGTTAC1040.11656579242322349No Hit
GTTGCAGGGCCACAAGAAAGACCTACAAAGACCACATGGGAGGAAAAAGAAA1030.11544496749607712No Hit
TCTATTGGGATGACTCAGAAATACCACATTTTTCTTAGTGACCTGTTTTACC1030.11544496749607712No Hit
CTTTTAGGGCAGGTGATACGCTAAGGCGTTTGGACTTTATTCTAATCATCAA1020.11432414256893073No Hit
GTGCCTGGGCTGTGGGTCAGGAGATCAGAAATGGTTCTATTTCAAGGTCTCT1020.11432414256893073No Hit
TTGTTAGGGAGCTTTATCAGTGTATACGATCATGTTAATGAACTGATTTGTT1020.11432414256893073No Hit
AGTCAAGGGGAGCTGATGTCAATGCTATGGACTGCACAAAAGTCTCAATGTT1020.11432414256893073No Hit
ATAATAGGGAGAGAAGCAGTCAGAGATGAGAGGATGGCAGCTAGCTGAAAGA1020.11432414256893073No Hit
TATATCGGGAGCTCAGCAAGGAAAGAATGGGCTCAGAAAAAGGAGGGCTGGC1010.11320331764178436No Hit
GTTATTGGGGATGCTGGATTGAGATAGACTGCAATGGGGAAGGATAGAAGTG1010.11320331764178436No Hit
ACTGCAGGGAAAGAAGGAGTCAAGACTGGCAATTGGCTGGATTAAGCTAAAC1000.11208249271463797No Hit
TTGCCTCGGGAGAATGTCTTGGTAGCACATGTAGGAGGCTGTACCTAATGAT1000.11208249271463797No Hit
GGTTGCGGGGTAATAGGAGCTGAATGATGACAATTTGGCTTTGTGTGCATTG990.1109616677874916No Hit
TACTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC990.1109616677874916No Hit
CTTCAAGGAGAACTACAAACCACTGTACAAGGAAATCAGAGAGGACAGAAAC990.1109616677874916No Hit
TGGGTAGGGCGCCCCTCAACAGGCATGACTCTGCAGAGATGACTCAGCACTG990.1109616677874916No Hit
CTATATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG990.1109616677874916No Hit
ATATGTGGGAACCGATTCCCAGATTCGCCATGTTCACATGCTCTCGTGCGGC990.1109616677874916No Hit
GCCCGCCTTGGCCTCCCAAGGTTCTGGGATTGAAGGTACGAGCCACCGCAAC990.1109616677874916No Hit
TCTGCATGGGCAACGGTGAGCTTAAAATATTCAGTAAACCATGCTATAAACA980.10984084286034522No Hit
AGCCATGGGCAATTCAAATATGTGTTGTTCAAGTGTCAGCTGTATTGAAAAT980.10984084286034522No Hit
GTTTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC980.10984084286034522No Hit
TCTTGTGGTGGGTACCTCTAAAGCAAATCTGCAGTGTTCCAAAGACTTTGGT970.10872001793319883No Hit
ATTTTCGGGTGCACTCTAAATTGGAGTCAGAGACACAAAATCAATTCCCAAA970.10872001793319883No Hit
GGTACCGGGCATCAGTCTGCAGTAATCCAGTCAATTAAAAATGGATTTCAGT970.10872001793319883No Hit
TATTAAGGGGATCTTGCCAAGGGTGCCCAATTCACTCCAATAAGATAAACTT970.10872001793319883No Hit
CCTAACGGGGAGCGCGTTCTTAGCAGTTGGCTTCATGGACAACTCATTAGTG970.10872001793319883No Hit
TCAGGGGTCCCTAAAATACTGCATGTGTGTGCATACATGCATTTTCCTGGGG970.10872001793319883No Hit
CTTAGAGGGCATAATGAATTCAAACAAATTCATGCCTATTAAAAGGAGGCTT970.10872001793319883No Hit
GACTATGGGTTGTGATGAAAATGCAAAGGAGCCCGAAAATGCACTTGACAAG970.10872001793319883No Hit
ATAATTGGGCTGTATCTCTAATGGATGGGCCATGCATGTTTCCATGCTGACA970.10872001793319883No Hit
TAGTATGGGAACCTTAAAAGAGCTAGAGTGGCCATATAAATATGAGACGACA970.10872001793319883No Hit
ACAGGCGGGGGGATCTGCGGCCGCACCGGCAGTGGGAAGTCCTCCTTCTCTC960.10759919300605246No Hit
TTACTGGGGGAGAATAATTATTTGATTGCTAGATGCAGCTTAACACCTACTG960.10759919300605246No Hit
TGCCACGGGCGGAAGCTCAGTGCTGTAGTCCAACTGTCCCTTGTGGGCACTG960.10759919300605246No Hit
CCAGCAGGGACTCTCAGATATCAAGATTTATTGTAAGGACAGAATAATTATG960.10759919300605246No Hit
ACACCAGGGTTGATGATTTGGCATCAATTCAAAGAGCAAATGATACCAAGAT960.10759919300605246No Hit
ATATACGGGGATAGCTTTTGCAGCCTATTTGCCCCAATTGTGTAAATACTAA960.10759919300605246No Hit
GCCCGGCTAGTTTTTGTATTTTTGGTACAGACGGGGTTTCGCCATATTGGTC960.10759919300605246No Hit
AAAGATGGGAAACATTAAATGTGTCAAGAAATCTTGGCAACAGAGACTCTGC950.10647836807890607No Hit
ATAACGGGGCAGCAATGCATCGAGGTATGAGCTATCAACTTTCTTCCTTTTT950.10647836807890607No Hit
AAAGGCGGGATTCGCAAGAAGGACTGGATGACTAAATCTGGGCTATCTCCGT950.10647836807890607No Hit
TGTAGAGGGAGCCCTTTCTTCTAAATCAGAATGTTGTCTTTTCCACAGATGG950.10647836807890607No Hit
CTAGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA940.1053575431517597No Hit
CTTACAGGGCAATTGTTTCCTTAATAGATTGTGGTTTTGGTGTGATATCTGT940.1053575431517597No Hit
AAAACTGGCATGGAATAAACCAGTAACTAATATTTCAGCTGTTGAAACAGAA940.1053575431517597No Hit
TGTCATGGGATTCTTATTTCTTTGTCTTTCCCTTAGCTTTAACCAAAACCCC940.1053575431517597No Hit
GCTTTAGGGACTTGTGAGGACTCACATAAATGCTGTTGTGGCAAAGATTCAG940.1053575431517597No Hit
TGCGCTGGGCATGAAGAGGTCCCTGGTATTGAAATCGGAAGCCCTTGCTGTA940.1053575431517597No Hit
AATGCTGGGGAAGACGTCAGAGTCACAAATTTAGGATGATGGCATAGTGTTG940.1053575431517597No Hit
CTACTGGGGGAAGTGACTCTTTCCAAGATGATATTAACCCAGAAAGTTTAGG930.1042367182246133No Hit
TCAGTAGGGAGTGGGCCATTCATTTCTATAGGCAAAGAAAGCTCTAGACAGA930.1042367182246133No Hit
TATGATGGGTCTCCACATTAGCCACTCAATTTCCAGTACCATCCTGGCGGAT930.1042367182246133No Hit
ATTGGTGGGACTACATCAATGGCAGTTTATTTTGTGAACATGATAGACCTAC930.1042367182246133No Hit
GGACATGGGGAAAATTGACGTTAAAGAAGTTTCTCCATTGTCTTACTAACAA930.1042367182246133No Hit
TCAGTCGGGCATCCACCATCCAGGATCACCATGGATATGGTCTGTGGTCTCT920.10311589329746694No Hit
ACTGGGTGGAGTATACAGTGGGAACTGGCTTGGTTAAGACACGAGGACATAA920.10311589329746694No Hit
TCCCGGGATGATTGTCACTGTCACTATCATCCTCTCATTGTCGTCATCACCA920.10311589329746694No Hit
AAGACCGGGGGAAAAAGAACTGCAGCAGGAACTTCAAGAATATGAAGTGGTG920.10311589329746694No Hit
CACAATGGCAATCCAAAAAGCACCTGCACAGCAAAGAAAGCAATCAACAGAG920.10311589329746694No Hit
ATCTAAGGGATAATTCAAATTACTTTTAGTTGTTTTATTTATAACTGTATTC920.10311589329746694No Hit
TCTTAGGGGGACGCTCTTCCGATCTTATGAGGGGGCACCTCCTCACCATGGA920.10311589329746694No Hit
AGTAATGGGCACCATGCCAGGCCTTTTGTGTTACTTTTGTAAGAGATGAAAT920.10311589329746694No Hit
TGGCGTTGGGCCGATCTTCTACTGGGTGCCAGACATTTATAGGTACTCAACT920.10311589329746694No Hit
AGACCAGGGGAATTTCGTTCTTAGTGCAGGTTACTTGTAGATTCTAGTCTTC910.10199506837032055No Hit
TATCTGGGGATAGGTAGTGACACACTGAAATTCTTATTTGTCCAATAATCTG910.10199506837032055No Hit
ACTGTTGGGGACGCTCTTCCGATCTACAATAGGGAACCCTGATTTATGTTCT910.10199506837032055No Hit
GCGACCGGGATTTGGTCAAAACCTGCCAGTGCAGCCTCTTCTTACAGACTAG910.10199506837032055No Hit
TAAGCAGGGCATAATTTTTAGTCTCTCATGCTATACCACTTAACAATTACTA910.10199506837032055No Hit
GAAGAGGGCAATTTAGGAATGCTCAATGTGCTGTTGATTCTTTCAATCCACA910.10199506837032055No Hit
ACCATAGGGCATATATACACAACCAAGGTGTGATCTGAATTTAATCCACATT910.10199506837032055No Hit
GTCGCAGGGGAGATGAGTCTCACCAACAGTGTGTAAGTCATTAACAGTCCTA900.10087424344317418No Hit
GATTCTGGGACCACTGCGGTGAGTGCGGCCTGGGCTTCACGCAGGTCTCGCG900.10087424344317418No Hit
TCTTAAGGGGATCTAATAGCGGGGGGCAGCGGCGGCGGCGCGAGCTTCGGCG900.10087424344317418No Hit
CCTAATGGGCTGCAGCACCTCCATGCAGAAGCTCCCTCTGAGGCAGGACTGG900.10087424344317418No Hit
GTCAAAGGGATAATGAATTTACTATTACCCATGTAATTGTGCCAAAGCAGTC900.10087424344317418No Hit
GCAAGCGGCCGCCTAACTTGGGGTTCTGTCTCCTAGGTACATCCCCACCGCA900.10087424344317418No Hit
AAATATGGGGACGCTCTTCCGATCTAACCCCGGGGGAGTAAAATGAGAAGTA900.10087424344317418No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)