FastQCFastQC Report
Sat 31 Dec 2022
SRR5847614.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847614.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79434
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAACGGAAATCTCGTATGC4260.5362942820454717No Hit
CTTATACACATCTCCGAGCCCACGAGACGAACGGAAATCTCGTATGCCGTCT4190.527481934687917No Hit
TTATAAGGGCTTGTGTATTCTAAAAATAAGAGCACAAAAACTTTGAAAGTAA1260.1586222524359846No Hit
AACTCTGGGAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGC1180.14855099831306492No Hit
GAACTTGGGACGTTATTTTACATTATTTTGTTTTTATTTTTTGTCATGTAAA1160.146033184782335No Hit
CTTGGTGGGGGTTCTACCAAGGCCCTTCCTTGAGCATTTATTATTTAAGTTT1130.14225646448624013No Hit
CCATAAGGGCGATCTTTTTAGGGGAAGAAGCAAAAGAAAAATTAAGAAAGTC1130.14225646448624013No Hit
TTTTCAGGGATTGTACCACTTTTAATTGCACTTATTATTGCCTTCAGTAAAG1120.1409975577208752No Hit
CATCATGGGCTGAAATTGACGTGAAGCACAGTAAATATGATTTGGTGTCTAT1080.13596193065941536No Hit
ATTAATGGGCTACTACAACCCTTCGCTGACGCCATAAAACTCTTCACCAAAG1060.13344411712868545No Hit
GAACCGGGGATAACTATTAATTTTCTTTTGAAAATTTCCCCTTTCTTTGTAA1060.13344411712868545No Hit
GGGTGAGGGACATTCAATTATGAGAGATAAACATGTAATCTCAAGTAAAAAT1040.13092630359795554No Hit
TTGTCCGGGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAAT1030.12966739683259057No Hit
TTAATAGGGTAGATATTCATGATCGCAAACATTTCCAGAGAATAATGTTGGG1020.12840849006722563No Hit
AGCTACGGGCGATCTCGAAGAGGGAAATAAAGAAAATTGGATGCAAGACAAT980.12337286300576579No Hit
GGCCTTGGGCAGCTTTAAAACTTAGCTGTCGTTCAGTTAAGCTTAAAGATAG970.12211395624040083No Hit
AGTAGTGGGGAAGATAACTGACAAGAAGTATTAAGAAAAGTCTTTAAGAAAA970.12211395624040083No Hit
TTGCGCAGGAGGAGTTATTCATATTTATGTTGACAAAAATTCAGCTCAGGGC960.12085504947503588No Hit
TCATCAGGGATTGGATAAATATTAGGTGGGCCTCTACTTGTATATTACCAGA950.11959614270967092No Hit
AATCCTGGGCTTAAATATATTGGGTTGATCTTTTGTTTTTTAAATTAATATT940.11833723594430595No Hit
ACGCATGGGAGTTTCCGTCGCCTTGACGACAAGACCACCAACGTGTCCTTGT930.117078329178941No Hit
CTCGAGGGGAAGAAAGCATAATCCATAGATGCTAAAACAATTAGGGGTTAAA920.11581942241357605No Hit
GGGTTTGGGATATTCTTTCTAGAAAGATGGAATTGATGTATTTTAAAATACT910.1145605156482111No Hit
TCTATTGGGATATTTGAGCAGGAGTCCTTTAGCAGATAGATGGGATGTCCTT910.1145605156482111No Hit
ACTGGCGGGAACTTTAATACTGGACAAAATATATGAAGCATCATTTTCAGGC910.1145605156482111No Hit
GCCCAGGGGGAATGTAAATTAGTTTAGCCACTGTGGGAAGCAGTCTGGAGAT910.1145605156482111No Hit
AGTAAGGGAGCCCAGGATTGCTACAAAAAGGTCCTTTGAGAAAATTAATTTG900.11330160888284614No Hit
CATGGGGCATGTAGGATAACTTTATCTGCCTCTTCTTCTTGCCATTCCCATC900.11330160888284614No Hit
CCACCTGGGGTCTCGCTGTTAGTGCAAGCTGATGGCTTTTGGATATGTTTTT900.11330160888284614No Hit
AATCACGGGTCTGCGCAGGGACCAATAAGAATAAACTTACACATACCAGAAT890.11204270211748117No Hit
GCTCTCGGGATGTTTCTTTGTTTACTTCATTTTTTCCTCTAATTATTCAAGA890.11204270211748117No Hit
GTGCTTGGGATTGAGATCAGAGATGCTAAAAGAGCTGCCAGTGACATCATTT890.11204270211748117No Hit
ATGTTAGGGCAGTGGAAGAATTTAAATTTGGCGCTACGATCAAAACTACTGA890.11204270211748117No Hit
TTCTGTGGGGGAGTACAAATTAGACTTATAGTCCTCCTGGAACTTGAGTTAT890.11204270211748117No Hit
ATCGCAGGGCTTATTCTTTTGTGTGTTTTGTTCGGTGAGAAAAAGAAATGAA880.11078379535211622No Hit
ATGCGGGTGAATCTAATAATGAGGAAACATTAGAAAAAAACCAAACTGAGGG880.11078379535211622No Hit
GCTCAGGGAATCTCATATAAGATAGAACATAATGATAAAAATGAGTGTTTGA880.11078379535211622No Hit
ACGTCAGGGCTATTTATTCTGTTGATAGTTTCTTTTGCTGTTCAGAAGCTCT880.11078379535211622No Hit
ATAATTGGGATACAGAAATTCCATGCCTGCATCCAGTTTTCAGCAGCAAAAG880.11078379535211622No Hit
TAAACGGGGTGTTTGATTGAATCTGGCATCATTTCTGAGACAATTCAATTAG870.10952488858675126No Hit
GTGACTGGGGGGAATTTATGATTAGAACATTTGAAATCCAGGCAAAGTTTTA870.10952488858675126No Hit
GCTTTAGGGATCCTGGGATGAAGTTGTTTCGTGCAGTCTATCCCTGTTTCCC870.10952488858675126No Hit
CTATGCGGGAAAATAACATTTCTTTTTGGTGTTTTAACACTTAATTTTATAA860.10826598182138632No Hit
TATAAAGGGAGCCCACTGGGGTATTGATTCTCTGAAGTGAGACTAATTCAAG860.10826598182138632No Hit
TGACACGGGCAGGAAATCCCAAAGTTTCCAAAGCTTTTTGTTTACAGAATAA860.10826598182138632No Hit
TGCATTGGGGAAATCTTACCACCTTTCATTTTCCCTCTGTTACCAAATTTCA860.10826598182138632No Hit
GGTAATGGGAGTGGAATAAAAGTTTTATGAATGGACTTTTCAACTACTTTCT860.10826598182138632No Hit
TGGGATGGGGTAGTAAATAGACACACTGGCTTAAATAAATTATTTCTGAGGA860.10826598182138632No Hit
TTTTAGGGGGACGCTCTTCCGATCTTGTAAAGGGCATTTCTCAAGATAAACA860.10826598182138632No Hit
TTAGCAGGAAATGAGTACAACTCTAGCATTTTGAAGTTCCAGTTGTAAATGT850.10700707505602136No Hit
AGATGCGGGGTTTATTGTTTAATTTATGCCCAAGTGAAGTTGTAAACTTATG850.10700707505602136No Hit
TACAATGGGATACCAATTTAGGGACATCGTGTTTTCCAAATCCTAAATGAAA850.10700707505602136No Hit
GCTCTTGGGTATCAACCAAATTCTGTAGGTGCAAACATATACCAGGGAATTC850.10700707505602136No Hit
ACTGCTGGGTCTTGCGTTGGGGTAAAAGATTGTGTAATAAGGTCTTCATGTT850.10700707505602136No Hit
ATGACTGGGGATGGATCTCTGTTAAAAATTTTTAGTGACATAGGAAAATGCT850.10700707505602136No Hit
AGCTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA850.10700707505602136No Hit
AGAATAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA840.10574816829065639No Hit
CAAGCAGGGAGATGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAA840.10574816829065639No Hit
GGCTCGGGGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGATTTTTTAC840.10574816829065639No Hit
CTATGGGAGATAATTTTCCTTTGGGCTGCATTCATTGTTTTATAACCCTCAT840.10574816829065639No Hit
TGAAATGGGAGAGGGGGTAGCATGTCCTACCCACTCATAAGGGCTTCTATTC840.10574816829065639No Hit
ATGGATGGGATCTCTGGGCTAATTTGTAAGATCTATGTTTTAAAACTCCTTA840.10574816829065639No Hit
CTAGTAGGGTGGCATATACCTGTAGCCCTAGCTGCTCAGGAGGCTAATGCAG840.10574816829065639No Hit
CGAGAAAGGGGCAGGCTCTCATCGAGGCTTATGTAGACATGAGGAAGATTGG830.10448926152529144No Hit
GACAAGGGCAATCCATTATCAACTAGCTATTTCACAGGATATATTCTGTCTT820.10323035475992648No Hit
CTGCGAGGGCTCACCAGAGGATCTTCCCTTCCTGGTCCTGCTTGTGCATATG820.10323035475992648No Hit
GTCAAGGGACTAAATGGTTGCTAAAAATTACATGTCTTAAATATTGTCTTAA820.10323035475992648No Hit
CAGCCTGGGTGACAAGAGCAAAACTGTCTCAAAAAAAAGAAAGCCACAAAAT820.10323035475992648No Hit
TGAGCAGGGCACCTGGATGCCTACGTGGGAAGGGACCTCGAATGTGGGACCC820.10323035475992648No Hit
TTATTGGGGGCAGTGGGGAGGGGGCTTCCCATTCAATAAATGTTACTGGCTG820.10323035475992648No Hit
TACTCCGGGAGTCATTGATATAAGGTATTTTGGATTCTCAAACACAAATGTT810.10197144799456152No Hit
TTTCATGGGAGAACAGATCTTTTACTTTGGCCGCTTGAAAAGCTAGTGTACC810.10197144799456152No Hit
ACTGTTGGGACACTATGGAAATTTGCAAATGCTACAAGTTAGGCATTCGTTT810.10197144799456152No Hit
GTGCGCGGGGGTTGCGCCGCGGCGTGAGGTGAGGTGTGAGTTGCCCTGGAGG810.10197144799456152No Hit
TTAGCTGGGGATCTAAAAGTGGGAGCTGGACCCAACTAGGCCATCATGAGTG810.10197144799456152No Hit
AAGTATGGGTAAGCTGAAATATATGCATGTAAAAACTTTGACATCTTTTTTT810.10197144799456152No Hit
AGAGAGGGAGAGAAGCGACATGTAAAAAACTGGTGGAAAAAGGTTTTGGATT800.10071254122919658No Hit
TTTACAGGGCGATCTAATTGAGGGATCTAAAATCATTTCAACAGCAGAAATG800.10071254122919658No Hit
AGTGCTGGGGTAGGATTACAGGCATCAGCCCACGGTGCCTGGACACCATGTA800.10071254122919658No Hit
GCATAAGGGTAAGTTCGAGGGCGGAGTGGTGATTGCCGCAGACATGCTGGGA800.10071254122919658No Hit
TTATCCAGGGGAACTATAGACTTTAGATGGTGAACTTGATGAAAAGTATAAA800.10071254122919658No Hit
CAGGTGATCCACCCGCCTCAGCCTCCCCAAGTGCTGGGATTACAGGCATGAG800.10071254122919658No Hit
GTAAATGGGAGAACATGAAATTCTGTTGGCAGAAGCAGCATTTTGAAGAAAT800.10071254122919658No Hit
TCTATGGGGATCCAGAAATATACCTAATAAGATTGAGTGAAAAATTTGAGTC800.10071254122919658No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)