FastQCFastQC Report
Sat 31 Dec 2022
SRR5847392.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847392.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131997
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCAAGAATCTCGTATGCCGTCT8000.6060743804783442No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCAAGAATCTCGTATGC6210.4704652378463147No Hit
TTTACAGGGGGCTCACCATTATCAATCATCAGAAAAAGGTGAATCAAAACCA2120.16060971082676123No Hit
TTTCTAGGGAATTTCCCAACTTTTGTCTTCAAATTAAAAAAGAAAATAGCAT1990.15076100214398813No Hit
ACCGGGGAGTTTGTTACACGAGGTCTGAGAGACAGAGGCAGCGTGTTTGAGC1850.1401547004856171No Hit
TCACTGGGAGGGCTGACAGCAAGCTGTTGGCATTATTCACATTTGGGTCCTG1720.130305991802844No Hit
GTAATAGGGAGGAGGGAGAGATTGGGAGGAGGGAAGAAAGTGAGGAATAGGC1710.12954839882724606No Hit
TCAAGCGGGGGAATTAATGGCAGATTAAACATTGCTGAAGAAAAAATATTTG1700.12879080585164815No Hit
TAAGCGCGGGGGGAGAAAGATGGCGGCAGCCGTGCGACAGGATTTGGCCCAG1670.12651802692485434No Hit
GCTTAGGGGTAGTTCATGCCTGTAGCTCTCTTCCTTTTGTTTCTCACCCTTC1640.12424524799806057No Hit
TAATACGGGTGCTCGGCAAAATAAACTATCAATAAACAAACAACCTATACAA1630.12348765502246263No Hit
ACAAACGGGATCCCAGTCAAAACCAATACTTTCTTTTCTTTATTTTACAAAG1610.12197246907126677No Hit
TGTAATGGGACAATACTATGAAATAGGTATTATTATTGCCATTTTCCTGTGC1600.12121487609566885No Hit
CTTCGGGGGAGTCCAGAAATGTGATGATAGTAGCTTGAGAAGAAAATGCATC1590.12045728312007091No Hit
ACTATGGGGGAGCTCTCAACTCCAAGTTGTAGATTTGGTGTCTTCCTTGTTC1590.12045728312007091No Hit
ATCACGGGATAGTTTACTATCAACCATAAAATGCATTTTATAATTTTGTAAT1580.11969969014447297No Hit
TCTTGCGGGATAGGAAATTCCTGTACCTTCTTGGCCCCCATAATGTGTTTTT1560.11818450419327713No Hit
GCTACAGGGGAGGACACGGGTCGCTGGCGGGTTAGGTGGCTGCGTGGCCGCC1560.11818450419327713No Hit
CAAGCTGGGAAAAGTAATTACTAAAAAGCACTTAATAGTCTAGAAATCCACC1560.11818450419327713No Hit
TGACTAGGGATATAGAATCCATCTGCAAATTAGGTTCCCCCCACTAGATGCA1560.11818450419327713No Hit
CAACAAGGGACAATTTCCTTTTTTCCATTCACCTGTCTTGATGTGCAGAAAA1540.11666931824208125No Hit
AACGCAGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGG1530.11591172526648334No Hit
CACTAAGGGGAAAAGATGTAGGAATCTTGAAAAGTGTTCTGACACACTATGC1520.1151541322908854No Hit
AGCAATGGGAATGTCATGAATGTGGCAGAGGCTTCACTCTGAAGTCACATCT1500.11363894633968953No Hit
ACTTCCGGGATCTGATTGTGGTTTTTGATTTGCATTTCTCAGATGAGAGTGA1490.11288135336409162No Hit
TCTGCGGGGACTATAAATGTGAATGGAATCATCTAGCTCCATTGTTCTAACA1470.11136616741289575No Hit
AATGCTGGGATTACAGGCGTGAGCCACTGCGCCCGGCCGAGAAATTTGAAAA1470.11136616741289575No Hit
TTCGCCGGGGACAAGAGCTTGATGAATGAAAAGAAGGAAGCCTGTGACAATT1460.11060857443729781No Hit
AATAACGGGATCTGGAAGCTGAAAAAAATAACCACAATAAGGAGATTACAGT1450.1098509814616999No Hit
CTCCTCGGGGAAGTGCCAGATTCTGTTTCATATGGAAGAGAGAATAATGACA1440.10909338848610196No Hit
AGAAAGGGGACACTGCATACACTTGACTAATTTCAACTGCCTATTGAAATCT1440.10909338848610196No Hit
CGTTCCGGGATACAAAATGAGCAATCATAAGGATTTGAGAATCTGTTAAAGA1430.10833579551050403No Hit
TACCAAGGGGATCTTTAGCCGGGGTTTGAAAAGATTTTAAGGGGTCACGGAC1410.10682060955930815No Hit
TGCATACGGGAGGTTTTAATAAATATCATTTCAGTTTTACAAGATGAAGAGA1410.10682060955930815No Hit
TATTATGGGACATTGGGGGATGCCAGAAATATATTGCTTAAGATTTTTGAAA1410.10682060955930815No Hit
TAGTATGGGCGATCTTTTGGCGGGGGGAGGGGCCAAGAGAAGGAAAACCCTG1410.10682060955930815No Hit
GCTAATGGGACTTGACACTTTTACTGCAGCTTCTAGTCTGTTAAGTGGTTTA1400.10606301658371024No Hit
AATTTGGGATTATCAAATTGAATTATTTCTTTTGTTCTTTTATTTTCCCTTC1400.10606301658371024No Hit
AAGCTTGGGATGCCAAACCAAAGAGCAGCTGCAGTACATTCGTTATTTTAAA1400.10606301658371024No Hit
TGGATAGGGAGATTGCTTAGAGAAAAAAATAAAATGCTTATCTTAAGGATAT1400.10606301658371024No Hit
GAACCTGGGACATGTGCATAGACGTTCCTGGGCTATGGAAGATAAAAAATCA1390.1053054236081123No Hit
TGCCCAGGGAGCCCTTCAAGGTCTGGCGCTAAGCTCATTTTCTCGCAGCTTC1390.1053054236081123No Hit
GAGTTAGGGGCCCCCACCGGACTTGGTCGCGCCCGAAGTGTAACACTTTCTC1390.1053054236081123No Hit
TACTGCGGGGACTCGAGCCAGCTCAAGCCCGCAGCTCGCAGGGAGATCCAGC1380.10454783063251437No Hit
ATACTAGGGCATCTTCCACACTAAACCTTTCTATGGGGGCAAGAAGCACAGA1380.10454783063251437No Hit
TAATGTGGGAGTGCATGGTTATTTTACATAGTAAGACCTGGAATTTTATGGG1380.10454783063251437No Hit
TAGTTAGGGAATTTAACCACTGCACAGTTTGTTCAGATAAGCTTTCCTTTCC1370.10379023765691643No Hit
GGTACTGGGAGCTGGTGTGATTGGAGCTCAGAAGCCACAATATGATATCTGG1370.10379023765691643No Hit
ACTACCGGGCTTTGTGTTTAATGGAAAATTTTCATTGACGTATCTTCAAACT1370.10379023765691643No Hit
GGCACAGGGGAATTAGAATAGTATTTTGAAAATAGTCAACAAGAAATGTAAA1370.10379023765691643No Hit
AGACGGGGATTGTTTCACATCCTTACACCCTTATAACAAGCTCCTCTCCCTC1370.10379023765691643No Hit
AAGCATGGAATAAAAAAATTATTTTAAATAATTAAACAGATTTGGAAAAGAC1360.10303264468131852No Hit
TTTAAGGGATTATATAGCAGTAAAAAATGGTGTTTTATCTTCTATATAATTC1350.10227505170572058No Hit
AGTTGGGGATTGTAATAGTTTAGATGAGAAATGATGAAGATTTATACTAAAG1350.10227505170572058No Hit
GCGTAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA1340.10151745873012265No Hit
AACTTTGGGGGGATGGGAGCCTTCTCCGGATCTTTTGTTCTTCTGCACCTCT1340.10151745873012265No Hit
GACTCAGGGCTCCAACACTTGACAAAAAACAAAACTGAAGCTGTGATTGGTT1340.10151745873012265No Hit
GCGTGGGAGAGTTCTATTTCTGCGTAATTAATTACCAGAAACTTGGTGCCTT1340.10151745873012265No Hit
GCATTTGGGAGTTACGGCAAACCTGAGATTTCAGCATAAAATCTTTAGTATG1330.10075986575452471No Hit
CGCCTGGGCAAGGAACTACACTCAGATGAAACCTCTGTGCTTATGTCTAGTG1330.10075986575452471No Hit
CTAAACGGGGAGTAGGACGTTTTTAACAGTGTTTCAGAGAATGGCACTTGCT1330.10075986575452471No Hit
AAGTTTGGGGTGAGCGGCGGGGTCGACTGACGGTAACGGGGCAGAGAGGCTG1330.10075986575452471No Hit
GTTCATGGGAGAGGCAGAGGCAGCATCCAGCGGCGGCGCCAGCAGTTCCAGT1320.1000022727789268No Hit
GCTTAAGGGATGTTGCCCTTTCTTACTCTCCAGTCATTTGCTGTGATCAAGC1320.1000022727789268No Hit
TTCCGTGGGCAAGCAGCCGCCTCTAACAAACACCATGGTCCGTGGAAGTTCA1320.1000022727789268No Hit
AGTCAGGGAGAGTTTTTAGCATGAAGCGTTGTTGAATTTTGTCAAAGGCCTT1320.1000022727789268No Hit
CGTTCAGGGGAGTCGCTATCGGAGGCCGCGCGGGTGGCTGAGCAGCGGTCTG1320.1000022727789268No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)