FastQCFastQC Report
Sat 31 Dec 2022
SRR5847305.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847305.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131897
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT3380.25626056695755023RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC2690.20394701926503256No Hit
TAACTTGGGGATAGCAGATGCTTTTCATTTTTAAACATAGAATTAAAATTGG1850.1402609612045763No Hit
TGCTGCTGGGACGAAAATTAATGTTTCAAAGAGCTGCAAGGAACTTTATATG1820.1379864591309886No Hit
AAAGCAGGGGTGATTGCTCTGGTGAGACTGAATGGACTTGCTGGTGAAATGA1740.13192112026808797No Hit
GACAACGGGAGTAAAGCTTTGTGCAATGATCGAGTCATCAACAGTATTCCTT1740.13192112026808797No Hit
ACTCCGGGGAACGTGCTGGGCATCATCCCCTATGCGGGCATCGACCTGGCCG1730.13116295291022542No Hit
AATAATGGGGATCTGTGCGCGGGGTACTATCCCTATGAGGCATAATTATAAC1670.12661394876304993No Hit
CACCGAGGGAAGTAAAAAATCATTTTTCCTCCTCCCTCCCCAAACCCCAAGG1640.12433944668946223No Hit
AGAAAGGGGAAACCAAGGGCGAGGGACCAAAGAGAAAGAAGTGACAGAGTAT1600.12130677725801194No Hit
ACTTAAGGGAATAATGGAAACACAACATACCAAAACCTATGGGATACAGTGA1600.12130677725801194No Hit
GCTGCTGGGGTGCCATTTTGTCATAGAATGTAAAAATTGGTTAACTTTACAA1590.12054860990014936No Hit
GACTATGGGCAGGAATCAAAGGATGTGGAAGAAAATGTTGGCCTCATTGTGT1570.11903227518442422No Hit
AACAACGGGCGATCTCCACATGGGGAAGAATGCAGGGCCCATTCAGCATGGG1570.11903227518442422No Hit
CACCAAGGGGGGCGCTGTGGAAATTGGGTCTTGGGCTGGGTGGCATCTGGCA1570.11903227518442422No Hit
CGCGTTGGGGCAAATTGTGAAAGTAATTAGTGACAAAATAGCAGCCTGCTCC1570.11903227518442422No Hit
TTCGCAGGGCTACCTGGGCAGTGCCAACATGGACTGGCGTTCACTGACCCAG1560.11827410782656164No Hit
ATCATGAGGCAAGCAACTACTTCACTGTATGGCCTGCAACCAACTTCTGCAA1560.11827410782656164No Hit
AAATTGGGGCGATCTTTACTGGGGGCCGGACAGAGGATCGCCTCGCTGGCCT1550.11751594046869905No Hit
TACACAGGGATGCCCACAGATTTCTTGCATGTTTCCAGTTGTATTTGATCTC1550.11751594046869905No Hit
CATTGGGGGGTGGAGGGAAGATTTCACTTCATTGTCTAGCCCAGAATCTTGA1550.11751594046869905No Hit
AACGCAGGGGCGAGAAGTGGGACGCACCAAACTGGAGGTCAAGGTGGTCATC1540.11675777311083647No Hit
TTTGCAGGGAAGAAGAAAGCCTGGCCCAAGATGTGAATAAGAGTGTAATGAT1530.11599960575297391No Hit
ATTATGGGGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGC1530.11599960575297391No Hit
GTAGGTGGGACGGCGGGGTAATGATGAAGCTTATGTTTATCATTTAAAAGAA1530.11599960575297391No Hit
TATAAAGGGGGAAGGTTCGGGAGGCTTCTGGAAAAAGCGCCGCGCGCTGGGC1520.11524143839511133No Hit
GTACCTGGGTACCTACACTTATAACAATAATAGCTATCATTTATTGGGTGTT1520.11524143839511133No Hit
GTGCACGGTGGTGGTTTGCACCAGGACAGTTGCTGTAGAGGAGGTGAACAAA1490.11296693632152362No Hit
TGTTACGGGCGCATTTTCCCATGGTGTATGACTAGTTCATCTCTTTCTTGAG1480.11220876896366103No Hit
TGAATCGGGTAGCACCATCACCCTTGAAAACTCTCAGTCAGTGTCATGAATG1480.11220876896366103No Hit
CAGTACGGGGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAA1480.11220876896366103No Hit
TCATTAGGGGGTTTAGCAAACTTCTAAATAGCCCATTTTAAGGGAGAACTTA1470.11145060160579848No Hit
GCCACCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGT1470.11145060160579848No Hit
AGCGAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGA1470.11145060160579848No Hit
GTCGATGGGGATGTAAACACCCGAGCCGGGCTCCAAGGCCCGGGAGGTCAGA1470.11145060160579848No Hit
TGAACCGGGCAGAGAAATGAACACATAATCATGCAAAAACCTGTACACCAAT1460.11069243424793589No Hit
AATGGGATTGTTTTAAAGTAAACCAGATCTCCAAATACCTATAAAACAAACC1460.11069243424793589No Hit
TCTGCTGGGATCAGCTGAGAGATTATTTGCTGCTGTTATTCAAAAGGCCATT1460.11069243424793589No Hit
CTATGCAAGGGAGTGTGGCTGCTGGAGGACGCTATGATGGGCTAGTGGGCAT1460.11069243424793589No Hit
CATTCCGGGAATGCCCAAGCTGATTCCCCCACTGAAAGCCCTGTTTGCTGGC1450.10993426689007331No Hit
CAGTTAGGGAGTGCTTAATATGTGTAAGGACTTCCTCTTTAAATTTGGTACC1430.10841793217434817No Hit
TATACGGGGCTGATAACCAGTGTAAGCTGGAGCTACAGGGCGTCAAGGGTGG1430.10841793217434817No Hit
GTGAAAGGGATGTTCCAGTAAGTTTAGCAACTTAGAGCCAAGACTGGTGGCC1430.10841793217434817No Hit
ACATGGGGACCTAGAAGGCATGTTGTTATTTTGATGAAACAAAACACAGCTA1420.10765976481648559No Hit
TGCTGGGGGTGTGGGACCGGCGCCATCGCTGAGCGAGCTGGATCTGCGGCCG1420.10765976481648559No Hit
GCTCCGGGGGATCAGGGATGCAGTACCACTTGCAGTCAAATGAATTCCTTCG1410.106901597458623No Hit
TAAGTAGGGGAACTTTTGGAAATTAATTTATACATAATGAAAGAACAAACTC1410.106901597458623No Hit
TAGATAGGGTGTCAGGATGCCTTTGTTAGGATCTGTATTTGCCCTTAATTTT1410.106901597458623No Hit
GGCATTGGACTTTTTTTAACTTGTTTTCATCTGTTTATCATGACTTTTTTAT1410.106901597458623No Hit
TACTCAGGGCTAAGGTAACTAGGCCCCCTCAAGAAAAAGTGGCATAGGGGAA1410.106901597458623No Hit
GGAGTTGGGCTTTGTTTTATATAATGCATTCTGCATTTTTAAGAAGTGTTCA1410.106901597458623No Hit
TCAGGTTGGGACACTTAGAATACTACAGCATAAATCTGTTAGCATTATTGCC1410.106901597458623No Hit
AGACGAGGGCCCTCCTCCTACAAGGTTTTGAGTTGGCTGCTGGTTAGCAAAC1410.106901597458623No Hit
GCTAGCGGAGGGCACGGTGGGTGGCAATAGTGAAAAGAAAACACCTTGTTTT1400.10614343010076045No Hit
ATTCTTGGGGCATGAACCTGGACATATTTTATAAATGGGGAAACTGAAATTT1390.10538526274289786No Hit
TTTGGCGGGGAAGGAAATTTACCTGTTCGAGGCACAATTCCTGATATGATTG1390.10538526274289786No Hit
TATTCTGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT1390.10538526274289786No Hit
AGACTGGGGGTGGAGTTTGCCATCCAGATTTGTTCCATATTAGTTTATCCTT1390.10538526274289786No Hit
CAGGCGGGGGGGGCTCGCGCCGCAGAGGCCGGTGAGGCGCCGGCGGCCACGC1390.10538526274289786No Hit
GGTTCTGGGTTCACCTCCCTGCTCATGGAACGTCTCTCAGTTGATTATGGCA1380.10462709538503528No Hit
TATAGTGGGAGTGGTATAAAAGACACGAATATCTCCTGGTCTATAAGGATAC1380.10462709538503528No Hit
CATCAGGGGATGTATCTTTAACACTTTTGAATAGAACAAACAGCTTTTCCAT1380.10462709538503528No Hit
TCTAAAGGGAGAAAGAAAAATATTTCTGTTCCTCAAGAAAACTTGCTACCCT1380.10462709538503528No Hit
TTACGACGGGGGCCAAGAACAAAAGAAAGATGCTTAATCTCACCACTTTTTT1370.10386892802717272No Hit
AAACTTGGGGTAGTTATCTGAAAATAAAAGGAGAGTGCTGAAGTTGAATATA1360.10311076066931014No Hit
GGACAAGGTATCTACATTCTAGAACATCTTCTTAATCCCTAAACAAGTGGAA1360.10311076066931014No Hit
ACTAACGGGTCTGCAGGGGCTGAGCTATAATTTGTTTTTAATGCAAAGACAC1360.10311076066931014No Hit
TCTGCAAGGGGAGAGAAAAATGGGGAGCATGAAAACAGAAAATTAGGGAGAA1360.10311076066931014No Hit
ATTGAAGGGCGATCTATGCATGGGGCATCAGGGATAAGGAAAGGTTAAAAAA1360.10311076066931014No Hit
ACTGAAGGGACTTTACCTTGCGGTGGTGGGATCCTGATGTCTTTCATCCATT1350.10235259331144757No Hit
TGTCTGAGAGAAAAACTGTGGTTATGAATATTTTTTACTTTTTGATAACTTT1350.10235259331144757No Hit
TAAGATGGGGCAACTTCAAATCGATATCAACTTCAACTAAAATGGTTAATGG1350.10235259331144757No Hit
TATATCGGGACAATCAGGTTCTGCTGCAGTTTGTTTATTGATGGGTATTTAT1350.10235259331144757No Hit
AAGACAGGGACTTATAATTGAGAAAGAATGTGGTGGGGGGAAGGGTGGTAGT1350.10235259331144757No Hit
GTGATGGGGGATGTCCTGCATCTAACGCGGTGTAACCCCCGAAGCCGAGCGA1350.10235259331144757No Hit
TATCAGGGATGGCACAAGGAACTGAAAGTAACTATTGGCTGGCTTAGGTGAG1350.10235259331144757No Hit
TGCACGTAGGGGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTAT1340.101594425953585No Hit
TTTCTTGGGGCTTCCTCAGGCGGCGGCCATGGCGGGACAGGAGGATCCGGTG1340.101594425953585No Hit
AAACTCGGGGCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAAC1340.101594425953585No Hit
ATGGCGGGCTCTTTAAAAGTGTTCTTTAACACTGACTACTAAACTTTAAGTT1330.10083625859572243No Hit
AACCTAGGGGACCTGTCCTGCAGCACTGGATGCTTTGTGAGTTGGGGATTGT1330.10083625859572243No Hit
GACAAAGGGGCCAGCCCTTTCTGGATTACTGATAGAAAATCATGCAAAACCT1330.10083625859572243No Hit
CAGTGTGGGATAACAAATACTCAGCAAATGTGAAACTTTATTTGCTCTTACT1320.10007809123785984No Hit
CAAAAAGGGTAAGAACACCAGTACCTAGCTATGAATATCATAGGCTGTGACT1320.10007809123785984No Hit
TCGCGAGGGAGTTGTTGGATTTATAACTTTATTGAGGAATTAATTTGATTCT1320.10007809123785984No Hit
TTACGTGGGTACTATATATGGTATAGACAAAGCATCAAACTATGTACAGGAA1320.10007809123785984No Hit
CGAGGAGAGGATTCAGTGATTGGTATGAGATGCAAGATGCTGGAATTACTTC1320.10007809123785984No Hit
GCTCAAGGGTGGATGAAGAGAAGAAAATCCGGCTTCGGTTGCAGATGGAAGT1320.10007809123785984No Hit
GAAACTGGGCTAGGGATAGTTCCTGCTATCTCTAGGAACATCTGTTTTTTCC1320.10007809123785984No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)