FastQCFastQC Report
Sat 31 Dec 2022
SRR5847277.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847277.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205666
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC6550.31847753153170677No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT5690.2766621609794521RNA PCR Primer, Index 23 (95% over 21bp)
TGGGCAGGGTACCCTTTAATAGAAATTTTATCTTTTGTTATTTTGGTTAATT3400.16531658125309967No Hit
GCTTTGGGACTGAAGACAAACTGATTCTGGGCTGTGAAGATTCTTCGCTAAT3120.15170227456166796No Hit
CTAATGCGGGGAGCATGGTATGTGTTTCCATTTGTTTGTGTCATCTGTGATT3040.1478124726498303No Hit
TTGCACGGGTAACATTTCCTGTCACTGTGTGTGGGAAAAATAGATATAACCA2940.14295022026003326No Hit
TTACACAGGGGATTTCACTTATCACATAGTATTTCACATAATATCTATTGTT2910.14149154454309415No Hit
AATTTAGGGCGATCTAAATGGAGGGCAGTCAAGGTGAAAATTAAGCTCAATA2770.13468439119737827No Hit
TTGCTAGGGACTAGGAATTGGAATCAAACAATGCCACCCCAAGCGTAATTTT2760.13419816595839856No Hit
ACGTAGGGGCAGGATTTTATAAATTGTACTTATATGTTAGTTATGTTTCTAC2700.13128081452452034No Hit
AGCTTGGGGACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT2690.13079458928554064No Hit
CTCCACAAGGGAAATGTGCTACTTTTTGATACTTACATTTTCATATGCTTTA2670.12982213880758123No Hit
AATACCGGGGGGCAGAGAGAAGTGGTCAAAATCTGGATATATTTTGGAGGTT2670.12982213880758123No Hit
CTGTCGGGTGTTGCAACATTTTTTAAAACACTGTAAAATACACCTGAAGTTT2660.12933591356860152No Hit
AAGCTAGGGTAACCCAAAGAGGGAGTCCTGGGAACCCCAATTTGAAGACAGT2650.12884968832962182No Hit
CTGCCAGGGGTGGCTTAAAATAAAGAAAATGCAGCAAAAAGAATGCTATAGA2650.12884968832962182No Hit
ATGTATGGGGAGGGCGAGAAGATCCGAAAGAAATACCCGGACCGGGTGCCGG2620.12739101261268268No Hit
TTTACTGGGAGAACTGGACTTCCTCCTCCGGGAAGCCTCCCTTGACCTTCCC2620.12739101261268268No Hit
TCATGAGGGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACC2610.126904787373703No Hit
CAGACGGGGATCTGAAACTGTGCTGTGAGTTACTTTGCAGTCTAAAAGCAAA2610.126904787373703No Hit
TTATTGGGGAAGCCTTCTCTGAAGTATTGAGGGAAAGTCTGCTAAATGGCTA2590.1259323368957436No Hit
ATCCCTGGGGCTGTGAAAGATGCCGCTTCCTTGTCACTTCAGAGAATGATGC2590.1259323368957436No Hit
CACTGGTGTTTATTACAGAGTTTGACATACATGGGTTCATTTGCCATTTATT2580.12544611165676386No Hit
GATGCTGGGATCTTTTGTAATGTCATTCATAGAAAACTGGTAAATGTGTTTC2570.12495988641778417No Hit
GTATGTGGGCGATCTGTGGACGGGCCTATCTCACGTGCAAAGACACACATAG2560.12447366117880447No Hit
TCAAAGGGAAGTTCTAGATGTTATTTATAGTCTACAGTGAATTTTGGTCTGT2560.12447366117880447No Hit
AGTTTGGGATTCTTTTTACCCTGAGTGAGCACTTTTCACTTTCCAGCTAGGT2550.12398743593982477No Hit
TTGCAAGGGCTTGTTTTCTGTGTAGTTTACCTTAAGTCCACTCTTGGCATTT2540.12350121070084506No Hit
ATCATCGGGGTAAGGGGCCAATTCTAAGTATTCTTCACGGCAGCCAGAAGTT2510.12204253498390594No Hit
TGTGCTGGGGCGGAAATTGTCTATAAGTAGGCATTTATTTCATGATTGATAT2510.12204253498390594No Hit
TTCACAGGGATATGAGGTAACTCACCATCACAAAAATGACCAAAAAACGTTT2490.12107008450594654No Hit
GCAGCTGGGTTAAAAGCTTTTCCTGGAAGATACCCATTTAATGCAAAAGAGA2490.12107008450594654No Hit
TATGATGGGGTACAATTAGTAGTAATTTGAAATGATGCCTATGGTTTCCTAA2480.12058385926696683No Hit
AAAACGGGGTATGAAAAAATGTTCAGTCTCACTAGTAGTCAGGAAAATACAA2480.12058385926696683No Hit
CACTCGGGACCGAGGAGATGGAGCTGTACGAGCTGGCGCAGGCGGCGGGCGG2440.11863895831104802No Hit
GCTCACGGGCACATCCACACTGATCTTACCCCATAATGAACTTGGCTCTGAA2420.1176665078330886No Hit
CACATCGGGACTGCGTATGTGTCTAATATTTGTAAAATAATTAGTTCAAAAC2410.1171802825941089No Hit
AAGCAGGGGAAAGAGTATATCAGAATCTTTTAAACAGGTAGCTAATTTTTTA2410.1171802825941089No Hit
TTCAAGGGGGGATATCAGACTGGGAATTTTTATTTGGGACTACATCCCAGAC2410.1171802825941089No Hit
AAAATAGGGGTCTTAGAGGAAAAAATGTAAAGGTAAGTAATTAAGCATATGA2390.11620783211614949No Hit
TATACAGGATGCTAAACGCAGCGTATACATAACATGAACAAGACATAAAGCA2390.11620783211614949No Hit
TTTGCTGGGGATGCCTCAGCCTCCCAAAGTGCTAGGGATTACAGGCATAAGC2390.11620783211614949No Hit
CAGCCTGGGAAACAGAGCGAAACCTTGTCTCAAAAAATCAAAAAAACGGGAA2390.11620783211614949No Hit
AAGGCTGGGACCTAACCCTTAGAAAATGAATGTTAGAAGGTGCCTGCCGAGG2390.11620783211614949No Hit
TGAGGTGGGATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA2390.11620783211614949No Hit
AAGTTAGGGGTATTCACCTTGGCCTCATGCAGTGCATATTTGCTGGTTGCCT2380.11572160687716977No Hit
AAGCTAGGGGATTCTCAGGGTGATGTAGGGGAAAGCATAAAGAAGGCTGAGT2380.11572160687716977No Hit
TCAACCGGGCTCTCAGCCCTCCTAATGACCTCCGGCCTAGCCATGTGATTTC2370.11523538163819007No Hit
AAATATGGGGGTAACCAGCAGGCAGCTGAGAGCAAGAAGGCACGTGCTCTGC2370.11523538163819007No Hit
TTTGTGGGGATGGTGAGTTGGTTTGGGAGAGGAATCTCCCAGATCCCCGTTC2360.11474915639921038No Hit
CCGCGAGGGTAAAATGCCAATTTTACTTTTTACTGTACTTTTTCTAGGAGAT2360.11474915639921038No Hit
TATGCCGGGAATCTAGTAATGGGACCAAGATAAGTAGAATTGTGTGAGAGGT2350.11426293116023067No Hit
ACGCGGGAGTACTGTATTAGGCATTAAAAGGATGAAGATGACTAAGGGAACA2340.11377670592125097No Hit
ACACTCGGGATGACTCCACATGAACCTTCACATTTGTTCGCTCATAATCTAC2340.11377670592125097No Hit
ATAAGGGTAAACGCAAAACTTTTGTACTTTATTACGAGTAAAGTGTAATGAG2340.11377670592125097No Hit
ACACTCGGGTAGAAAAAGATCCTGGAAGTTTATTTGATCTGACATGTTTTTG2340.11377670592125097No Hit
TCGTCAGGGGGACTTGATGTTTGCGGTTTGAGAGGCAAGAAAATAAAATAAC2340.11377670592125097No Hit
TGGCCCGGGGAAGTAACCCATCAAAATATGCTCTGCAGTGATTCCGCTTAAT2330.11329048068227125No Hit
ACTTCTGGGCACATCCAGTTATGATATGCTTGTAAGCAGGCATTAATTCTTA2330.11329048068227125No Hit
ATCAGCGGGCTGGAGGATAATGAAAAGTCCGTGTTTTGAAGTTTTCAGATTA2330.11329048068227125No Hit
TCTTAAGGGAACCTGAACATGAAAAAAATTTTATTTGTGCTTAATCAAATTA2320.11280425544329155No Hit
TATTATGGGCAAAGCAGGTGGCATTTTACTGAACAATTAAATGTTTGAAGTA2320.11280425544329155No Hit
TTCCAGGGGAATTCTATGTACAGTTGACCTGTTTAGAAGTTGTATTGCCATT2320.11280425544329155No Hit
TGGTTTGGGATTCTAACTAACTCCTTTTCTCTTTAAAAAAGCAAACTTCATT2310.11231803020431184No Hit
GCTAGAGGGCATTCCTCATGGGTTATGAGTTGCTCCACAGGATTCTGGATGT2310.11231803020431184No Hit
TTGCTTGGGCAGCTACATGATATGCTCATCTACATGCACGTTTGTATGTGAA2310.11231803020431184No Hit
CTGCTGAAGGGTTACAGGGTCTGTTTCACCCAGCCCGGAAAGTCAGAGATGT2310.11231803020431184No Hit
GCGTGGGGTGCTATGAACAATAGCCTCTTAGAAGATAATAACAAAAATTTCC2300.11183180496533215No Hit
CTTTACGGGCTAGAAGAGAAAATTCCTAATATCCTGTCTGCATTTCCTTTCC2300.11183180496533215No Hit
TAGGCTGGGATCACACCAAAACCTCAGGCTACAAAAGCAACAATAAATAAAT2300.11183180496533215No Hit
GATTTAGGGGAACTAATAAAATTTTATTTCTTAAATATAAATTTGACCTAAA2290.11134557972635242No Hit
GACTTGGGGCAGGAAACATGATATAAACAAAATTTACATAAGCCTATAATCT2280.11085935448737272No Hit
GCTAACACCTGGCAACCGCTGATTTTTTTTACTGTCTCCATAGTTTTGCCTA2280.11085935448737272No Hit
GATAGAGGGATTGTTGAAGACGTGACTTCATAGCAATATATAGAGAATAAAT2270.11037312924839303No Hit
TTAGCAGGGGAACGATACGCCTACTGTGACCATTAAAGTTGTAACTAATTAA2270.11037312924839303No Hit
TGCATAGGGGGTCAATTGATGTGATTATTTTTGTTAAATGTTGTCTTGTGCC2270.11037312924839303No Hit
ATGTTGGGGCAGAGAGGAATGAGAAGAAGCAGCAGATGGGCAAAGAGTACCG2270.11037312924839303No Hit
TTTTTAGGGATTTAATAGAATTATGCTTAGATAAGATGCTTTTTTTCATTGA2270.11037312924839303No Hit
TTGCCACGGGATCTCAGATAATATATTCTAATGATGAGAATAACTACTAAAA2270.11037312924839303No Hit
GGCCATGGGCTGTGCCGTTGTACCTTTCCACAGCAAAGTGTAAGGAATCCAG2260.10988690400941332No Hit
CGTACGGGGGGCCTTGTAGGACTGACCCCTGTGCTTGCTGTCACGTTTAAAG2260.10988690400941332No Hit
TTCGAGGGGCTTAGGTAGGGGTGAAGTCTCAGGGTATGGCTGAATTATAGTG2250.10940067877043362No Hit
GACTATGGGATAACCGTAGTGGATCTACCAATACACCCAATGAGTGGCATAC2250.10940067877043362No Hit
TAGTTTGGGTAACAACAGAACATCAAAGTATGAGGCAAACACTGAAAGAAGT2250.10940067877043362No Hit
GGTCAGGGAAATGTTTCTTTCCATTTTGCTTTGTTTTTCTATATCGATCTAG2240.1089144535314539No Hit
GCAACTGGGTGCCTTGGGTTATTCCTGAAAGCTTCCTAATTGGTACCTGCTG2240.1089144535314539No Hit
GCTGCTGGGGAACACACGTGTGTTATATTTACTTCTACATTGGCAACATGAT2240.1089144535314539No Hit
AGACATGGGCTTGCTGCACATCAGGAGATCTAGAAAGGAGACTTTTCTTCCT2230.1084282282924742No Hit
CAGCTGGGGAATGAATGAATTAATCAATGAATGGAGAGACAAGGAGAATCAG2230.1084282282924742No Hit
CGACTGGGTGGGCAAAACACAATTATTTTGGGGCTCTGAGATAGAGAAGTGA2220.1079420030534945No Hit
GATCTGGGCCTTGGATTGGTAGTATAGTGAAATGATTAGGGCAGGGCTTTGA2210.1074557778145148No Hit
TAATAAGGGGTCTTCAAATTTAACCCATTAAACCAGTTAACCTTATTTCATA2210.1074557778145148No Hit
GCAAATGGGTCTCCTAGTGATAATGCTCCAAGTCTATGAACTGTTAAATCAG2210.1074557778145148No Hit
TTGCCTGGGAGCTTAATTAGATACTGATAGATATACCTAGCAATATTTATAG2200.1069695525755351No Hit
AAATGAGGGGACTAAAGAAATTTTCCTAAAGGACCCCATCATTTAAAAAATG2190.10648332733655538No Hit
TTGCAGGGTATAAAATTTTAGCCCACATTTCAGGCTCCGTAAATCATTTGAG2180.10599710209757568No Hit
ACTGTTGGGCGCTCTTCCGATCTGCCCACGGGACGGGAATAGTTTTCAACGT2180.10599710209757568No Hit
GGAATTGGGCTGAGAACAGCCCATTTAAAAATTTCTAAATTGTGAAAAAATC2180.10599710209757568No Hit
GAGGCGCGGGTAACAAAAAAAATCAATGAAACCAAAAGCTGATTCTTTGAAA2170.10551087685859598No Hit
AACCGAGGGATCATAATTGCTTGGGGAGTGATTTTGGAATACACCAGAATAA2170.10551087685859598No Hit
AAAGCAGGGATCTCAGGCTTGTCGTCAGTGTACACAGAGCTGCTCATGAAGC2160.10502465161961627No Hit
ACATCAGGGACACTTGTTTTTCATTGCTTTAATTGATCAAATGATAGGTAAA2160.10502465161961627No Hit
TAGTACAGGGGAATGAACTAAGGATTAACTGAACCATTTATATATAGTACTT2160.10502465161961627No Hit
AGTCTAGGGAGCATCAGCAGGATGCCCTTTGGACTGAAAAACTCCAAGTCTT2160.10502465161961627No Hit
TACTGTGGGATAGCAAAACCTTGTCTCTACCAGTGCACCTGTGGTCCCGCCT2160.10502465161961627No Hit
ATATAAGGGAAGAAACTGAAGTTTAAAAATATTTGTGATCCAGAGTGATTCA2150.10453842638063658No Hit
AGCTATGGGATATTTTACCATTTCATGATAATTTATTTTAAGCCATTTTGAG2150.10453842638063658No Hit
AACTTAGGGTAAGTGATATCAGCCTGTCAGAAAGAACAGATACTGTGTGATT2150.10453842638063658No Hit
ATTCTCGGGGACTTGGCAATGCAGGCTCTTTTTTGGTTCCATATGAACTTTA2150.10453842638063658No Hit
GAAAAGGGGCTCCAGCCTAGGTGACAGTGAGACTGTATCTCAAATAAATAAA2130.10356597590267716No Hit
CGAATAGGGAGGCCTTATTTCATATGTTTCCTCAACTGTACAATGAACTATT2130.10356597590267716No Hit
GACAAAGGGAGAAAAGAGCAAAATGTGAAAAGACACAAGACTCTCAGGTATA2130.10356597590267716No Hit
TGGCCTGGGGGACCAGTACATCAGAGAGCAACGCGAGAAGGATTCTGTCATG2130.10356597590267716No Hit
GTACTAGGGGGAACACATGAGCATGTAACAGAGCCAGGAACCCTACTGCAGT2130.10356597590267716No Hit
CCAGCCGGGAACAGCTGGCATTTCAGTAGAACCATGGAGGAGCTGGTTCATG2130.10356597590267716No Hit
GATTCAGGGATCTATTTTAAATATATTTAGATTATAATTGCCTGGGGGTTGG2120.10307975066369746No Hit
GGTACTGGGATTTGAATGATGAGACTACCTCCAAGCAAATTAAATCCATGCT2120.10307975066369746No Hit
GCTCCTGGGAGGTCTTTTAGGTTTAGATTTCATACATCTCTTCATGTTAGCA2120.10307975066369746No Hit
TCAAAAGGGAATTCAGAAAGATTCTCTGGGTTCCAAGCAACATGGTATCACA2120.10307975066369746No Hit
CTGTTCGGGATCTCAATACAGAAGAGAAGGCAAAAATTGCATTTTAAAAACT2100.10210730018573805No Hit
CTTAAGGGGATCCTGATGCTTAATATATGAAAGGTAGTTTTTAATTCAAAAG2090.10162107494675833No Hit
TTGAGGGAATCCATGCTGCGTGCCTGTCCTATAATTGCCCATATAGTCATCC2090.10162107494675833No Hit
AACTCAGGGAGGTGAGAGCTGCTGCGGGTCACCTCATATTTATTTATCCCTT2090.10162107494675833No Hit
ACAATGGGATGTTATGATACTTTAGTTCTTAGAAATGACAGGTTAAAAAATG2090.10162107494675833No Hit
CTATTAGGGAGTGGGGAAAAGTAGAAAGCTTTTCTCCTGGGATCGAGAACAA2080.10113484970777863No Hit
AAACACGGGACAGTTCTCTACAAAAAGAACCAATGAACCCAATAGGAACAAA2080.10113484970777863No Hit
CGTAAAGGGTCTCCAAGTCCTTCCAGGGCAGTCAGGGGCGTGCCTACCTCTT2080.10113484970777863No Hit
TGAAGAGGGACCCTAAAACACAGAGATCTCACTGACCCGTTGCCCTGTAACC2080.10113484970777863No Hit
TGGGCGGGGACAAAATTGTTAAATTACTAGTAATCCTTACCTAAGATTATTT2080.10113484970777863No Hit
ATTTTCGGGATCTAAGGAAATTGTAGTTCCATCCCTTCTATTTTTAGTCTTT2080.10113484970777863No Hit
TTGTGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2080.10113484970777863No Hit
TGTTGAGGGTTGGATGGATGGATGGAGGGATGGAAGGAGGGAGGGAAGGATG2080.10113484970777863No Hit
TGGCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2070.10064862446879892No Hit
ACAACTGGAAATGTGCTGGGTCACACCTGAAGTCAGCAGGGCTCTGGGCCTC2070.10064862446879892No Hit
GTGAGAGGGGGTGGGCGGAAGAGGTGGCCCCGGATGCTGCGGCGCCCGCTGG2070.10064862446879892No Hit
AAATGTGGGTAGAGAAGTCACCTCAAGATTGGTGACAGATCTTCTCATGTGA2060.10016239922981923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)