FastQCFastQC Report
Sat 31 Dec 2022
SRR5847186.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847186.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105871
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGC5420.5119437806387018No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGCCGTCT5400.5100546891972306RNA PCR Primer, Index 34 (100% over 21bp)
AAAACTGGGATTAAGAAAAGACTAGAAAAGCAAACACAAAGCACACTGTGAT1900.17946368693976633No Hit
GAGGCGGGCGGATCACTTGATGTCAGGACTAGCCTGGCCAACATGGAGAAAC1640.1549054982006404No Hit
AGCCCTGAGCCAAATAGTAGAAAAGCTTGTGTTCTTTGATTCAACCCATTTT1530.14451549527254867No Hit
TCCTCAGGGATGTAGTAGATGGAACTTCCCCCAGAGCCCCAGGCCAATGGGG1530.14451549527254867No Hit
AGCACCGGGATTGGTTTTGGTAATCGATGTGGAAAACCCAAAGGACCAAGAG1520.14357094955181304No Hit
TTACCTGGGCAAAGGACATGATCTTGTTCCTTTTGATAGGTGCAGCAAACTA1500.1416818581103418No Hit
CTGCAATGGGGTTCAAGTACGTCTCAGGGTCTTCAGCCGAGAGGAGGGCAGC1500.1416818581103418No Hit
TTTTATGGGAGGAACAAGCTGGCTTCTGTGATTAGGTGAAGGGATGGGGGAA1470.13884822094813498No Hit
AATACTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1450.13695912950666378No Hit
CACGAAGGCTGAGGATCAGAACGCAGCAACCCATCTCATTCGCCACGCTGTG1450.13695912950666378No Hit
AGTCTTGGGGTTTAAGAGAGAAAGGCGGAAGAAATGAAATAGAAAAGCAAAA1450.13695912950666378No Hit
AAGTAGGGGACAAAAAAAGAAGCAGGATTGCCCTTAGGTTAGAGAATAGAAA1430.13507003806519255No Hit
TCATGCGGGAAACCAGGCTTACCGTGAATTAAGATCCAAATGAAAGAGGAGT1430.13507003806519255No Hit
TCTCAGGGGTAAGAAATATAGTTTACTTTGTAACCTCGTGCATTTATATACA1420.13412549234445695No Hit
GGTTATGGGAATGAACCTAACCTCATTCTTTGTATCTTCAGTGTTGAATTGT1400.13223640090298572No Hit
GCTGTTGGGGCACCAGAAGATGTCTGCATTACTCATTGCTAAAAATGTGTAG1390.13129185518225012No Hit
AACGTAGGGTGAAATAATTAATGTTGGCAAATATGAGAACAACAAAGTCAGC1370.1294027637407789No Hit
GCATAGGGGACATCACAATAATATATAATTCAGTCTCTAGTTTCCCTAGAGT1360.12845821802004326No Hit
ATGCAGGGTTTTGATAAACTTTGCAAGAAAAGCCCTTACCTCTAATTGCATT1360.12845821802004326No Hit
TATGGTGGGATAAGTAATATAGTTAACAAGCAAAAAAAGTGCTGCCACCTTT1360.12845821802004326No Hit
ACGAACGGGATGAAATTATGCATTACCACATTCAAACACAAATCCATTGTAA1340.12656912657857203No Hit
TTTTGAGGGCTCCTAACTTTGTCTCCTATTATTCTCTCCTGGGCTTTGTATA1340.12656912657857203No Hit
CATAGAGGGAGCAGATGCTGAGGCAGTTCATTAAACCAGGCCTCAGCTTCAG1320.12468003513710083No Hit
CCACCACGGGGGGTGCTTCTCAGTGGGAAGGTGTAAGGAGGGGCTTGCTAAG1320.12468003513710083No Hit
TAAGGGGGATTCATGAAGTGAAGCAGAAAGGGTCTGTAAGGAACATTCTAAA1320.12468003513710083No Hit
TACGCAGGGGCAGATTAGGATGGCCTAATGGTAAAACATCAGGCACATTCTA1320.12468003513710083No Hit
CAGTTTGGGTATTTATGCCTTCTAACTTATGTTTTGTAATATGTGGTGAGAT1310.1237354894163652No Hit
TTAGAGGGGAAGATTTACAAGTGTGATGATCTCTGGCAGAAGCCTGCATAAA1300.1227909436956296No Hit
GGTACGGGTGGTAGTCCATAGGTAGAATGTGTTTTCAAACTCTGAGGATGGG1300.1227909436956296No Hit
AGGAGTGGGCTGAGGACTTGAATTTACAGTGCATCAGATTTGTTGCAAATTT1300.1227909436956296No Hit
GTTAGCGGGTATTTGCTGATTTTAAATTAGAAAATCATTACTAATTACTGAG1290.12184639797489397No Hit
TAACAGGGAGATTTTTGTATTGATTTGCAAAATGCAGATTATATTTGATAGG1290.12184639797489397No Hit
AACCTGGGTGACAGAGCGAGACTTCGTCTCAAAAACAAACAAACAAACAAAA1290.12184639797489397No Hit
TAACATGGGGAAGAGACAGTGCTCTGCCATGATGACAGGGCACTGCTAGGGC1280.12090185225415837No Hit
GTGAATGGGGTAAGCTTTTGGGCTCCACAAAACCTGGATTTGCCTCAATCTC1280.12090185225415837No Hit
GAACAAGGGCGATCTGCAAGTGGGGACCTCAGGTGATCCGCCCGCCTTGGCC1280.12090185225415837No Hit
CTAAGTTCGGGGCACAAGGGCTGCTTAACACATTCCTATGCTACAAAAGACA1270.11995730653342274No Hit
AGCAATGGGTGATCAACAATATTCTTAGGTTCTTGGTTACTTTGCCCCAGGC1270.11995730653342274No Hit
CGTATTGGGAAGAAATTTAAACTCTACGTTTGGTTTTCACATACAGCAGCTC1270.11995730653342274No Hit
ATGCGCGGGCTCCTTCTGTCGCCCAGGCTGAAGTGCAGTGCTGTGATCTCGG1260.11901276081268714No Hit
TTGCCAGGGTGCCCTTTTCCCTCCATGGATTCATTTCTATCTATTGCACTCT1260.11901276081268714No Hit
CAACAGGGGTTTTAACTACAGGATTTTATACTTTTAATATTATTTGTTATTC1260.11901276081268714No Hit
CGTGGGGATGTGGGTCCACTCATATGCTGTTTTTTAGGAAATATGCTAGAAA1250.11806821509195153No Hit
GTCGAAGGGATTGTACTGAAAGTACCAGAAACTTTGGCTCTGCTTCTTAGCA1240.1171236693712159No Hit
TACCGAGGGACGCTCTCCGATCTGGCTACGGGAAGCACATGGCCTCCCATCT1240.1171236693712159No Hit
ATAGAAGGGGACGCTCTTCCGATCTAGCATGGGTGTCCCTTGGTGTTTTGTG1240.1171236693712159No Hit
CTTGCAGGGCTGAGTATGGGATCTAAGGTTTTAAAATAATTTGGTTTGAAAA1240.1171236693712159No Hit
ATTGTGTGGCGCAGCCGCAGCAAGAGTGAGAGGAGCAGAAAGCGAGGCAGCA1240.1171236693712159No Hit
GCGATTGGGATGGTTATCTTGTTTCTGTTAGAAACAAAGTTGTATATAGAAA1230.1161791236504803No Hit
AAACAAGGGATAGATAAATGATGACTAATGGGAACGTAATGCATGTCAAAAT1230.1161791236504803No Hit
ATGTCAGGGGCCCACCCACATAATGGAGAGTAATCTGCTTTACTCAATGTCT1220.11523457792974467No Hit
GCTGCTGGGACTCTCTACAGGACACATTTTACATTCAAATATACAAGTAGGT1220.11523457792974467No Hit
TCAACTGGGCTACTCCTCCTTATTCCTTTATGTATATCTGTATTTCCATTTG1220.11523457792974467No Hit
TTATATGGGGACGACCCGAGGTCGTAGGTGGATCTCCTCACAAAGGAGTATT1210.11429003220900907No Hit
TGTGCCTGGGGACAATAAATTCAACACTTTAGATGAAATGAGCAAAAATTAT1210.11429003220900907No Hit
GTAGACGGGGACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTGG1210.11429003220900907No Hit
GTTTTGGGGAAGATTAGACTAAATGATTTTCACAAAGCACATTCAAGGTTTT1200.11334548648827346No Hit
CAACGGGGAGGAAAATACACGTATATTAAGTCAAATGTACATCTAAAAATTT1200.11334548648827346No Hit
ACAATAGGGTACAAGCGGGTGCTGCTGGGGCCCCTGCCACCGGCCGTGGACC1200.11334548648827346No Hit
CTTCTGGGGAGAGTTACCAAAGGCTTGTTTTTTCTTGAGCAGTTGAAAGGAG1190.11240094076753786No Hit
GCTTTGGGGAATGAAACTCCAGAAAGGACATGTATCACTGGCAGCAGAATTA1190.11240094076753786No Hit
TCTCCAGGGGTTCCCTGGGTTCTCTTTTATTGGATTCCTACTACAGGACAAT1190.11240094076753786No Hit
CTTAGTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1180.11145639504680224No Hit
AATAATGGGGGTAAGGCTGTGGGCAAATAGGCAATCATATATTGCTGGCAGG1180.11145639504680224No Hit
TAATCAGGGAGGAGCTCTGTTATGCTAAAGGACTGCAGTTTTTTTATTACTG1180.11145639504680224No Hit
GTCATGGGGATGGGAGTTCCACAAAGAGAAGACAGAAAAATGAATGAAGAAT1180.11145639504680224No Hit
TTTCGCGGGGACAGAATTAGATTCTCAGTTACCACAATGTAATACTCATAAT1180.11145639504680224No Hit
CGCGCCGGGACCTAGTCTGACTAGAAGTATTCCACTTCAAGGTCTGAAGTAG1170.11051184932606663No Hit
TAGTCTTGGGAGCGCCACATCTCCTGAGATTATTTGTACGAATTGGAGCAAT1170.11051184932606663No Hit
GGCTGTGGGAAGTTATTTTTGGAACATTTCCATTAGCCATTCAGATCTGCCA1170.11051184932606663No Hit
GCAATAGGGCATGGTTGGCTACCAGCCCCAAGGGGACAAGGCCAACTTCTTC1170.11051184932606663No Hit
TATTTAGGGGACCCTGGCCAGCTATGAAATCCTGAGTTGGAAGGGTTAAAAT1170.11051184932606663No Hit
GTACGGGGCAGAAGCACGGGCACAGGAGCATGTTAAAGGCCCTGAAAAGACC1170.11051184932606663No Hit
GCGACTGGGGGAAGACGACAGCAGTGAGGCGGACTCCCCGAGCTCCTCTGAG1170.11051184932606663No Hit
TTGCTAGGGGCTGCAACTTCCGGGAGGTGCTTGTGTGCCTGGTGCGGGAGCT1170.11051184932606663No Hit
ATTGCAGGGTTACTTTCATGGTTTCAACCATTATTTTATGTCAAATAACTCC1170.11051184932606663No Hit
ACTCGCAGGGGGGGCTGGTGGAGGCGGGGTCGAGATGGCGGCGCCTTTGAGG1170.11051184932606663No Hit
ATGCTGGGAGTCATGTGACCTATCAACATAGTTGCCTTAACCCTTCACTTCA1170.11051184932606663No Hit
GGCTTTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1170.11051184932606663No Hit
CTAGATGGGAGACCGTGTTTGCATTGAACATCAACATGAATATTCTGATACT1160.10956730360533103No Hit
TGTCTGGGGAATGAAAAGAAGATGTAGATAAAAATTCCATAGGAAGATAGGA1160.10956730360533103No Hit
TAATATGGGACCCTACCAAAGAGAGGGAGTTGACTGGCTTTTAAAAAGTATT1160.10956730360533103No Hit
TAAGCTGGGCTGTAACGGGGGTTAGATGCGCCACGGTTTCGGTAGCGACGGT1160.10956730360533103No Hit
CTGCGTGGGAAAGAGCTGGGGCAGAGGCAGGAAGCAGGGGCCCTCCTGGCAG1160.10956730360533103No Hit
TGCGCAGGGTGCTGTGTTACGTCATTCATTTTCTTCTGGTTCCTTAAGTTTT1160.10956730360533103No Hit
TGCTATGGGTGAGCAGGGAGCTGGGAGAAATAAGGAGGACATGAGTACATGG1160.10956730360533103No Hit
CTAGAAGGGTCACTTGCTGAGGTGCAAAAATTCTTAAGACTTCTGTTTGAAA1150.1086227578845954No Hit
TTTGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1150.1086227578845954No Hit
TTCCCAGGGACACCGTAGCGGCGCGCGAGCGGGCCGGGCGGGCGGCCGAGTT1150.1086227578845954No Hit
CAGTTAGGGATTCAAATTACTTGGAGTATTCTTTCTGAGAAGCATTCTGATA1150.1086227578845954No Hit
GTTGGGGGTGGTATAAAAAAATGAGTATAACCATAGGTTTTGAACAAATTTC1150.1086227578845954No Hit
TCGCGAGGGTGGGGCCATCTGATCCTGAACTCTTCTTTTTTGGGAGATTTTT1150.1086227578845954No Hit
TAACTCGGGGAGACAGCGCCGGGAAGATGAGCACGGAAGGTGGTGGCCGTCG1150.1086227578845954No Hit
GAAGCTGGGATCCCCATCAAGCTACCAATGACTTTCTTCACAGAATTGGAAA1140.1076782121638598No Hit
AGCCAGGGGGAAGTTAAACAGTATGCTCCTGAATGACAAGTAGATCAATGAA1140.1076782121638598No Hit
ACATTTGGGTGGAATAGGGCTTACTCACCCCTTCATGGAGGCTCGGGCAGCT1130.10673366644312417No Hit
GCAACAGGGTCCTGTGTAGGCTTGGGTTATTGTGTATATTTGTGTCTTAAAA1130.10673366644312417No Hit
AGTCCTGGGGCAGGCAAGAGTGCTGGAGGGCGGCAGCGGCGACCGGAGCGGT1130.10673366644312417No Hit
GTAATTGGGCAGAATGCACAGCCAAGTAGTAAATAAATGAGAGAGAGTGACC1130.10673366644312417No Hit
TACACCGGGTACATACATGACTGATTATATAAAAAGTTTTTACTCTGTCAAG1130.10673366644312417No Hit
TCATGTGGGGGAGTGGAATGCAAAATTAAATGTTAGAGTTATGGAACCCGAT1130.10673366644312417No Hit
TTAGCGGGGATACGATGATAGTCAGCCTAAATGAAATCCCTGAAGCATTTTA1130.10673366644312417No Hit
GTGGCGGGTCTGTTTGTTGGTTTGTTTGTTTCTTCCACGTAAGGAAAAGTAG1130.10673366644312417No Hit
CGTTTGGGGAGATCAAAGCCAGGGAAAGCAATCAGAAGCCAGATTGCATTCT1130.10673366644312417No Hit
TTACGAGGGAAACTCAATGTCCATCAATAGAGGAATGGCTAAATACATCTTG1120.10578912072238857No Hit
AATGTAGGGATCTGTCGGTGGGGAACCCATTCTTCCAGCTTCTTCTAGTGTT1120.10578912072238857No Hit
TCACAGGGCAATACTAGCATACGTTTTTCTTAATTGTTATCAGCATTGACCA1120.10578912072238857No Hit
TTATGCAGGGGGAATTAAAAAATATGGTCCCTTTGTTGCAGACTTTGCTGAT1120.10578912072238857No Hit
TGTGGGGCATTGAGGTTCATCAATGAATTTGTACATTTCTAGTTCCCTTTGG1120.10578912072238857No Hit
ACGCCTCGGGACAAAACAGACTTTAAACCAACAAAGATCAAAAAAGACAAGG1120.10578912072238857No Hit
TTACATGGGATTAATAGGCTTGCATTTCTTTTCCTAAATCTTATTTAGGCTA1120.10578912072238857No Hit
TACGGATGGGACCCCTGGGATGCAAGGACGGTTCAACATATGCAAATTAATA1120.10578912072238857No Hit
CTACACGGGGCAAACGGGAATTCACCTTTCACTTCAACATCAGTTTTAAAAA1120.10578912072238857No Hit
CAAAGAGGGCCGCCTCGGCCTCCCAACGTGATGGGACTATAGACATGAGCCA1110.10484457500165296No Hit
TATTCCGGGAAATGAGTACAACTCTAGCATTTTGAAGTTCCAGTTGTAAATG1110.10484457500165296No Hit
ACTTATGGGCCTCTCAGCCTGAGTAACTGGGACTACAGGTGTGTACCACTAC1110.10484457500165296No Hit
TTGCCAGGGGGACTGGGCACCAATGCTAGTTTCGGCAGCACACTTGGCACCA1110.10484457500165296No Hit
ACTTTCGGGGTCCATGGAGTCAGGGCAGCCGGCTCGACGGATTGCCATGGCG1110.10484457500165296No Hit
AACGCTGGGGCTGGGGCTATTATCTCTATTTGGCAGATGAGGAAACCGAGGT1110.10484457500165296No Hit
AACATCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1110.10484457500165296No Hit
AGACCAGGGGGCCCATGATGTGTCTCCGAGTGGAAAAGACTGGTTGGTGGTG1100.10390002928091734No Hit
TTACATGGGTATCTTTGAGTTAATTAATCTCACTAGTGTGGATAATATAATG1100.10390002928091734No Hit
ATTAGTGGGCAGCCTGGCCAACACGGTGAAACCCCGTCTCTACTAAAAATAC1100.10390002928091734No Hit
CGCTCAGGGGTTCCAGAGGGAAGATATACCACAAAATTACTCAAAAATTTTT1090.10295548356018173No Hit
TGCGTTGGGCACTCTACCAGTACCAGTTTCCTGTATTGGTCTGTTTTCATGC1090.10295548356018173No Hit
GAGTATGGGTAGGGGCTGACTTGTGTTGTGATTATTCCAATTGTGGGCTTTG1090.10295548356018173No Hit
TGTCAGGGGGGCTAAAACAAAATTTAATACTGTAAGCAAGTATAAATTTTTT1090.10295548356018173No Hit
GTTGCCGGGGCTCTCATTTGCCACAGGTTGGCCAGCTTAAGTATGAAGGGTG1080.1020109378394461No Hit
ATCGCAGGGGGTGTCAGTGAGACCTTGCAGAAGCTGAACATACATGTCCATA1080.1020109378394461No Hit
GTTTATGGGAAGCAAATTATTTTGAGACCTTTTGCTGGTAGACAAAAACTGA1080.1020109378394461No Hit
TAAGCGGGTTTCCCTGGGAACCCCACTTCTCTCTGTGGCTGGCTTGGTTGTC1080.1020109378394461No Hit
AGACTTGGGATATGTGGTTACTTAAGCATGGAAGCCATTGTGGGTATGTTTC1080.1020109378394461No Hit
ATTAAAGGGAGAATCCAGGTGCACGCTGTTAAAATTGTCAATGTGGATGATT1080.1020109378394461No Hit
ATGTAAGGGACACACATACACACACACGCACAAAGAGGACTGACTAAAGATG1080.1020109378394461No Hit
CATAATGGGGCAGACATACAGAAAGCTCTGTGTGATCAATAAGGGAGTGTCT1070.1010663921187105No Hit
GTCACTGGGGTAATAAACTTATTAGAAATTGGTGTTTGTGATAGCATTTTAC1070.1010663921187105No Hit
CATATAGGGGGGCTGGCAGTGCGGGCAGAGCCCGGCTGAGAGGGGCGGCCCT1070.1010663921187105No Hit
GACTCTGGGGTCACAGATGTGAAGTCGATTTCACTTTATAACTAACCAGTCC1070.1010663921187105No Hit
AAATTCGGGATTACTGATTTCAAATTTTTGACAAGCTGCTCATATCTTTAGG1070.1010663921187105No Hit
TTTAACGGGAATGCCTGCTGGGAGCTCTACTGCCTGGAACACGGCATCCAGC1070.1010663921187105No Hit
AGAACAGGGCAAATGGCCATCGGCCACAGCTCAGAGCAGCCAGTGGAGAAGA1070.1010663921187105No Hit
TGTGAAGGGCCAAGGAAGAAAACACATTGTGATGGGTTGATATGTCTCCTAA1070.1010663921187105No Hit
GTCATGGGGATATTCCTTTGAGCATTGCCGTTTTCTAGACAGACAATCCGAA1060.10012184639797489No Hit
AACATGGGGCTAAATTTGTATTGTTAATTTCTAATGTATTTATTTTGAGATG1060.10012184639797489No Hit
CTTCGGGGGGAAGACAGTGAGACCAAAGGATAAAGAGAGGCAGAAAGTGTTG1060.10012184639797489No Hit
GGGCCTGTGGAACGTCTATAGAAAAAATGAGTCAAACAGAGAATATGCAGGG1060.10012184639797489No Hit
TAAAAGGGGCATTTATCTAGAAGTTCTGAAATTTTAGGTGTGTGTATGTATA1060.10012184639797489No Hit
TACGCGAGGGAGCCTGTCTGACTACAAAGGAAAATATGTTGTGTTCTTCTTT1060.10012184639797489No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)